A5070960301- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Optical Coherence Tomography Applications
- Microfluidic and Bio-sensing Technologies
- Lipid Membrane Structure and Behavior
- Bone Tissue Engineering Materials
- Orbital Angular Momentum in Optics
- Optical Polarization and Ellipsometry
- Spectroscopy and Quantum Chemical Studies
- Dental Implant Techniques and Outcomes
- Advanced Fluorescence Microscopy Techniques
- Identification and Quantification in Food
- Protein Interaction Studies and Fluorescence Analysis
- Advanced biosensing and bioanalysis techniques
- Supramolecular Self-Assembly in Materials
- RNA Interference and Gene Delivery
- Bacterial biofilms and quorum sensing
- Bee Products Chemical Analysis
- Essential Oils and Antimicrobial Activity
- Bacillus and Francisella bacterial research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Gold and Silver Nanoparticles Synthesis and Applications
- Optical Imaging and Spectroscopy Techniques
- Vibrio bacteria research studies
University of Edinburgh
2021
University of Nottingham
2012-2020
The University of Tokyo
2018-2020
Park University
2015
Raman spectroscopy is an increasingly popular technique in many areas, including biology and medicine. It based on scattering, a phenomenon which incident photons lose or gain energy via interactions with vibrating molecules sample. These shifts can be used to obtain information regarding molecular composition of the sample very high accuracy. Applications life sciences have included quantification biomolecules, hyperspectral imaging cells tissue, medical diagnosis, others. This review...
The variable configuration of Raman spectroscopic platforms is one the major obstacles in establishing spectroscopy as a valuable physicochemical method within real-world scenarios such clinical diagnostics. For real world applications like diagnostic classification, models should ideally be usable to predict data from different setups. Whether it done by training rugged model with many setups or primary-replica strategy where are developed on 'primary' setup and test generated 'replicate'...
Various toxic compounds disrupt bacterial physiology. While bacteria harbor defense mechanisms to mitigate the toxicity, these are often coupled physiological state of cells and become ineffective when physiology is severely disrupted.
We describe a multifocal Raman micro-spectroscopy detection method based on digital micromirror device, which allows for simultaneous "power-sharing" acquisition of spectra from ad hoc sampling points.As the locations points can be rapidly updated in real-time via software control liquid-crystal spatial light modulator (LC-SLM), this technique is compatible with automated adaptive-and selective-sampling spectroscopy techniques, latter has previously been demonstrated fast diagnosis skin...
We investigated the feasibility of using spatially offset Raman spectroscopy (SORS) for nondestructive characterization bone tissue engineering scaffolds. The deep regions these scaffolds, or scaffolds implanted subcutaneously in live animals, are typically difficult to measure by confocal techniques because limited depth penetration light caused high level scattering. Layered samples consisting bioactive glass foams (IEIC16), three-dimensional (3D)-printed biodegradable...
We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy.Multiple laser foci, generated using liquid-crystal spatial light modulator, are individually used both trapping and excitation of spontaneous spectroscopy from trapped objects.Raman scattering each focus is spatially filtered reflective apertures on digital micro-mirror device, which can be reconfigured flexible patterns at video rate.We discuss operation the instrument, performance...
Spectral depth-profiling of optically turbid samples is high interest to a broad range applications. We present method for measuring spatially-offset Raman spectroscopy (SORS) over length scales by incorporating digital micro-mirror device (DMD) into sample-conjugate plane in the detection optical path. The DMD can be arbitrarily programmed collect/reject light at spatial positions 2D plane, allowing spatially offset measurements. demonstrate several geometries, including annular and...
Raman micro-spectroscopy (RMS) is a non-invasive technique for imaging live cells <italic>in vitro</italic>. However, obtaining quantitative molecular information from spectra difficult because the intensity of band proportional to number molecules in sampled volume, which depends on local concentration and thickness cell. In order understand these effects, we combined RMS with atomic force microscopy (AFM), that can measure accurately profile cells. Solution-based calibration models RNA...
Advances in consumer display screen technologies have historically been adapted by researchers across the fields of optics as they can be used electronically controlled spatial light modulators (SLMs) for a variety uses. The performance characteristics such SLM devices based on liquid crystal (LC) and digital micromirror device (DMD) technologies, particular, has developed to point where are compatible with increasingly sensitive instrumental applications, example, Raman spectroscopy....
Abstract In this study, we compared the depth discrimination and speed performance of multifoci Raman hyperspectral imaging with reference standard a single laser point confocal mapping. A liquid crystal spatial light modulator was employed for generation beams, digital micromirror device used as software‐configurable reflective pinhole array. The patterns foci array can be rapidly changed without requiring any hardware alterations. Confocal different distance‐to‐size ratios were tested...
Using phantom samples, we investigated the feasibility of spatially-offset Raman spectroscopy (SORS) as a tool for monitoring non-invasively mineralization bone tissue engineering scaffold in-vivo. The samples consisted 3D-printed scaffolds poly-caprolactone (PCL) and hydroxyapatite (HA) blends, with varying concentrations HA, to mimic mineralisation process. were covered by 4 mm layer skin simulate real in-vivo measurement conditions. At concentration HA approximately 1/3 that (~0.6 g/cm3),...
Cellular plasma membrane deformability and stability is important in a range of biological processes. Changes local curvature the affect lateral movement lipids, affecting biophysical properties membrane. An integrated holographic optical tweezers Raman microscope was used to investigate effect gradients induced by optically stretching individual giant unilamellar vesicles (GUVs) on lipid packing segregation cholesterol bilayer. The spatially resolved analysis enabled detection phase...
We report a new approach in tip-enhanced Raman spectroscopy (TERS) which TERS-active tips with enhancement factors of ∼10−5× can be rapidly (1–3 min) produced situ by laser-induced synthesis silver nanoparticles at the tip apex. The technique minimizes risks contamination and damage during handling provides feedback control, allows prediction performance. show that TERS this enable measurement spatially resolved spectra self-assembled peptide nanotubes spatial resolution ∼20 nm.
Richard Dluhy opened a general discussion of the paper by Duncan Graham: In your example heterogeneous solution-based assay for multicomponent analysis, what is concentration target fungal ssPCR DNA that used, and how do you manage kinetics reaction such t
While Raman hyperspectral imaging has been widely used for label-free mapping of biomolecules in cells, these measurements require the cells to be cultured on weakly scattering substrates. However, many applications biological sciences and engineering polymer substrates that often generate large signals. Here, we discuss theoretical limits signal-to-noise ratio spectra presence signals how optical aberrations may affect measurements. We show can obtained using automatic subtraction...
Nonlinear optical microscopy allows for rapid high-resolution with image contrast generated from the intrinsic properties of sample. Established modalities, such as multiphoton excited fluorescence and second/third-harmonic generation, can be combined other nonlinear techniques, coherent Raman spectroscopy, which typically allow chemical imaging a single resonant vibrational mode Here, we utilize ultrafast laser source to obtain broadband spectra on microscope, together approaches same...
The spectral resolution of broadband Fourier-transform coherent anti-Stokes Raman spectroscopy is limited by the maximum optical path length difference that can be scanned within a short time in an interferometer. However, alternatives to exist which bypass this limitation with certain assumptions. We apply one such approach using interferometers delay line (low Fourier resolution) and large (high resolution). With method, we demonstrate closely spaced vibrational bands possible...
Understanding and quantifying the temporal acquisition of host cell molecules by intracellular pathogens is fundamentally important in biology. In this study, a recently developed holographic optical trapping (HOT)-based Raman microspectroscopy (RMS) instrument applied to detect, characterize monitor real time molecular trafficking specific species (isotope-labeled phenylalanine (L-Phe(D8)) at single level. This approach enables simultaneous measurement chemical composition human...
Abstract Improved understanding of the mechanism nutrient's uptake can enable targeted manipulation nutrient sensing pathways in medically important pathogens to a greater degree than is currently possible. In this context, we present use spontaneous Raman microspectroscopy and coherent anti‐Stokes spectroscopy visualize time‐dependent molecular interactions between protozoan Acanthamoeba castellanii host human cells. Human retinal pigment epithelial (ARPE‐19) cells were pre‐labelled with...
Duncan Graham opened a general discussion of the paper by Pavel Matousek: Regarding temperature measurements, how did you do them? Stokes/anti-Stokes measurement? The values are not what I'd expect and seem lower. What was wavelength power laser calculate
An integrated atomic force and polarized Raman microscope were used to measure the elastic properties of individual diphenylalanine (FF) nano- micro-tubes obtain quantitative information regarding inter-molecular interactions that define their mechanical properties. For tubes, co-localised spectroscopy measurements allowed calculation Young's shear moduli (25 ± 5 GPa 0.28 0.05 GPa, respectively) contribution hydrogen bonding network modulus (∼17.6 GPa). The π-π between phenyl rings,...
ABSTRACT We present a combined holographic optical tweezers and confocal Raman spectroscopy instrument which allows fast, flexible, interactive manipulation with molecular measurement of interacting live cell systems. Multiple laser foci created using spatial light modulator are simultaneously used for trapping spontaneous spectroscopy. To enable measurements high spectral resolution, digital micro-mirror device was to generate reflective pinholes matched each trap. demonstrate this unique...
Due to the high flexibility and throughput, Spatial Light Modulator (SLMs) have been widely used in optical microscopy spectroscopy for various applications. In this talk, I will present some example applications of Raman using two types SLM, LC-LSM (liquid crystal-spatial light modulator) DMD (digital micromirror device). Several modalities can be achieved by and/or DMD, such as multi-foci spatially offset (SORS). The SLMs allow system flexible efficient collecting signal, requiring no...
Multiphoton microscopy utilizing pulsed near-infrared (NIR) laser pulses has facilitated many developments in the biological and materials sciences. excitation fluorescence (MPEF), most notably two-photon (2PEF), allows high-resolution 3D sectioning with greater penetration depth tissues due to NIR [1]. Second harmonic generation (SHG) sensitive imaging of non-centrosymmetric inversion symmetries (e.g. collagen) [1], surface third-harmonic (THG) provides contrast at interfaces strong...