A5066969742- Spectroscopy Techniques in Biomedical and Chemical Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Photonic Crystal and Fiber Optics
- Advanced Fiber Optic Sensors
- Optical Coherence Tomography Applications
- Spectroscopy and Chemometric Analyses
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Optical Network Technologies
- Protein Interaction Studies and Fluorescence Analysis
- Advanced Chemical Sensor Technologies
- Inhalation and Respiratory Drug Delivery
- Advanced Fiber Laser Technologies
- 3D Printing in Biomedical Research
- Renal function and acid-base balance
- Quantum Dots Synthesis And Properties
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Optical Sensing Technologies
- Advanced Biosensing Techniques and Applications
- Nosocomial Infections in ICU
- Streptococcal Infections and Treatments
University of Edinburgh
2016-2024
Centre for Inflammation Research
2016-2018
Queen's Medical Centre
2016-2018
Engineering and Physical Sciences Research Council
2016-2018
The Queen's Medical Research Institute
2017
International Union of Pure and Applied Chemistry
2014-2016
University of Strathclyde
2013-2014
Recent studies have clearly demonstrated that Raman and surface enhanced scattering (SERS) spectroscopies are information rich, non-destructive techniques for the monitoring of subtle intracellular changes. However, despite sophisticated applications these in cell there still remains a lack accompanying 3D images. Herein we demonstrate first time combined SERS imaging simultaneous confirmation cellular inclusion multiple component detection nanotags. We also report on elucidation nuclei by...
Lectin-functionalized silver nanoparticles have been successfully designed for use as molecular imaging agents to investigate carbohydrate–lectin interactions at the surface of mammalian cells, using surface-enhanced Raman scattering (SERS). Carbohydrate-lectin are key many cellular processes and responsible controlling an array interactions. In this study, lectin-functionalized were used detect expression carbohydrate species interface. The demonstrated three different lectin distinct cell...
Previously unobtainable measurements of alveolar pH were obtained using an endoscope-deployable optrode.The sensing was achieved functionalized gold nanoshell sensors and surface enhanced Raman spectroscopy (SERS).The optrode consisted asymmetric dual-core optical fiber designed for spatially separating the pump delivery signal collection, in order to circumvent unwanted generated within fiber.Using this approach, we demonstrate a ~100-fold increase SERS signal-tofiber background ratio,...
Dysfunctional intracellular enzymatic activity is believed to be an underlying cause of a myriad diseases. We present the first use surface enhanced Raman scattering (SERS) as detection technique capable reporting specific enzyme. Careful choice reagents allowed preparation high resolution cellular maps highlighting conversion commonly used ELISA reagent 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), by wild type β-galactosidase enzymes. Further, through co-addition X-Gal...
Full exploitation of fibre Raman probes has been limited by the obstruction weak signals background fluorescence sample and intrinsic signal delivery fibre. Here we utilised functionalised gold nanoshells (NS) to take advantage surface-enhanced spectroscopy (SERS) effect enhance pH responsive spectrum 4-mercaptobenzoic acid (MBA). However, is still dominant. Using photon arrival time-resolving capability a CMOS single-photon avalanche diode (SPAD) based line sensor, recover SERS without in...
Measuring Raman spectra through an optical fibre is usually complicated by the high intrinsic scatter of material. Common solutions such as use multiple fibres and distal optics are complex bulky. We demonstrate single novel hollow‐core negative‐curvature (NCFs) for surface‐enhanced spectroscopy (SERS) sensing using no optics. The background emission from silica in NCF was at least 1000× smaller than a conventional solid fibre, while maintaining same collection efficiency. transmitted pump...
The exploitation of fibre based Raman probes has been challenged by often complicated fabrication procedures and difficulties in reproducibility. Here, we have demonstrated a simple cost-effective approach for sensing pH through an optical fibre, employing wax patterned filter paper-based substrate surface enhanced spectroscopy (SERS). Through this method, high reproducibility between fibres was achieved. In addition to pH, it possible extract fluid samples containing P. aeruginosa further...
Duncan Graham opened a general discussion of the paper by Pavel Matousek: Regarding temperature measurements, how did you do them? Stokes/anti-Stokes measurement? The values are not what I'd expect and seem lower. What was wavelength power laser calculate
Dysregulation of receptor tyrosine kinases (RTKs) has been shown to correlate with cancer cell proliferation and drug resistance. Thus, monitoring the activity RTKs at a chemical level could provide new biomedical insights methods assess efficacy. However, direct kinase is challenging most commonly relies on in vitro techniques such as Western blotting ELISAs. Herein, we report development gold nanoparticle-based surface-enhanced Raman scattering (SERS) sensor, which allows real-time...
We demonstrated hollow core negative curvature fibres (NCFs) for Raman sensing. The background emission from the silica in NCF was at least 1000× smaller than a conventional solid fibre while maintaining same collection efficiency.
We are developing the next generation of optical fibres for endoscopic imaging and sensing. Our fibre bundle is fabricated from OM1 PCVD graded index preforms made telecommunications market. Whilst having a lower numerical aperture, performance shown to be comparable current state-of-the-art commercial microendoscopy fibres. In addition, we negative-curvature fibres, which exhibit ultra-low silica Raman background. Measuring spectra through an usually complicated by high intrinsic scatter...
In healthy humans, the physiological state in distal lung alveolar acinar units is tightly regulated by normal homeostatic mechanisms. Pulmonary abnormalities such as chronic obstructive pulmonary disease, that are characterized recurrent cycles of inflammation and infection involving dense infiltration myeloid derived peripheral blood cells, may result significant perturbation baselines physiology addition to host tissue damage. Therefore, ability quantify monitor (e.g. pH, glucose level,...
We present a spectroscopic system and an optical fibre probe which enable the full exploitation of temporal evolution spectral information weak Raman signal against background fluorescence intrinsic Raman. The consists single multimode CMOS single-photon avalanche diode (SPAD) line sensor capable resolving histogramming arrival times photons for 256 pixels simultaneously, offering improved to compared non-time resolved measurement modality. capabilities are tested standards such as...
The alveolar space forms the distal end of respiratory tract where chemoreceptor driven gas exchange processes occur. In healthy humans, physiological state within alveoli is tightly regulated by normal homeostatic mechanisms. However, pulmonary abnormalities such as chronic obstructive disease may induce significant perturbation baselines physiology well cause host tissue damage. Therefore, parameters (pH, glucose, oxygen tension) provide a key biomarker innate defence. Here, we discuss an...