A spectroscopic assay based on surface enhanced Raman scattering (SERS) using silver nanorod array substrates has been developed that allows for rapid detection of trace levels viruses with a high degree sensitivity and specificity. This novel SERS can detect spectral differences between viruses, viral strains, gene deletions in biological media. The method provides diagnostics characterization generating reproducible spectra without manipulation.

Substrates consisting of silver nanorod arrays with an irregular surface lattice (i.e., random nucleation sites) and varying rod lengths were fabricated by oblique angle vapor deposition method. These evaluated as potential surface-enhanced Raman spectroscopy (SERS) substrates using trans-1,2-bis(4-pyridyl)ethene a reported molecule. SERS activity was shown to depend upon the length nanorods. The Ag nanorods average 508.29±44.86nm, having aspect ratios 5.69±1.49 exhibited maximum enhancement...

Aligned Ag nanorod arrays have been fabricated on glass substrates using an oblique angle vapor deposition (OAD) method. The surface morphology and structure of the rods were studied by scanning electron microscopy, transmission X-ray diffraction. Individual nanorods found to be primarily cylindrical, albeit with irregularly shaped protrusions rods. enhanced Raman scattering (SERS) response as a function length was investigated trans-1,2-bis(4-pyridyl)ethene probe molecule at excitation...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantitative external reflection infrared spectroscopic analysis of insoluble monolayers spread at the air-water interfaceRichard A. DluhyCite this: J. Phys. Chem. 1986, 90, 7, 1373–1379Publication Date (Print):March 1, 1986Publication History Published online1 May 2002Published inissue 1 March 1986https://pubs.acs.org/doi/10.1021/j100398a033https://doi.org/10.1021/j100398a033research-articleACS PublicationsRequest reuse permissionsArticle...

Despite the requirement for a functional signal sequence in protein export, little is known of conformational properties and membrane interactions these highly hydrophobic amino terminal extensions on nearly all exported proteins. The Escherichia coli λ phage receptor was studied phospholipid monolayers by circular dichroism Fourier transform infrared spectroscopy; peptide shown to prefer an α-helical conformation when inserted into lipid phase. However, interaction with surface without...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantitative determination of conformational disorder in the acyl chains phospholipid bilayers by infrared spectroscopyR. Mendelsohn, M. A. Davies, J. W. Brauner, H. F. Schuster, and R. DluhyCite this: Biochemistry 1989, 28, 22, 8934–8939Publication Date (Print):October 1, 1989Publication History Published online1 May 2002Published inissue 1 October 1989https://doi.org/10.1021/bi00448a037RIGHTS & PERMISSIONSArticle...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFourier transform infrared spectroscopic studies of the effect calcium ions on phosphatidylserineRichard Dluhy, David G. Cameron, Henry H. Mantsch, and Richard MendelsohnCite this: Biochemistry 1983, 22, 26, 6318–6325Publication Date (Print):December 1, 1983Publication History Published online1 May 2002Published inissue 1 December 1983https://pubs.acs.org/doi/10.1021/bi00295a043https://doi.org/10.1021/bi00295a043research-articleACS...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIn situ measurement of the infrared spectra insoluble monolayers at air-water interfaceRichard A. Dluhy and Donald G. CornellCite this: J. Phys. Chem. 1985, 89, 15, 3195–3197Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/j100261a006https://doi.org/10.1021/j100261a006research-articleACS PublicationsRequest reuse permissionsArticle...

Hemoglobin gene expression in non-erythroid cells has been previously reported activated macrophages from adult mice and lens cells, recent studies indicate that alveolar epithelial can be derived hematopoietic stem cells. Our laboratory now produced strong evidence hemoglobin is expressed by type II (ATII) Clara the primary producers of pulmonary surfactant. ATII are also closely involved innate immunity within lung differentiate into I Reverse transcriptase-PCR was used to measure...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIn situ FT-IR investigation of phospholipid monolayer phase transitions at the air water interfaceMelody L. Mitchell and Richard A. DluhyCite this: J. Am. Chem. Soc. 1988, 110, 3, 712–718Publication Date (Print):February 1988Publication History Published online1 May 2002Published inissue 3 February 1988https://pubs.acs.org/doi/10.1021/ja00211a008https://doi.org/10.1021/ja00211a008research-articleACS PublicationsRequest reuse permissionsArticle...

Polarized surface-enhanced Raman scattering (SERS) and UV-vis absorbance spectra were measured for a nonplanar Ag nanorod array substrate prepared by oblique angle vapor deposition. The anisotropy of the SERS polarization was shown to differ from that polarized absorbance. maximum intensity observed in direction perpendicular long axis nanorods, while strongly along array. Analysis data showed molecular orientation not cause anisotropic scattering. Rather, primarily attributed lateral...

Human enteric virus infections range from gastroenteritis to life threatening diseases such as myocarditis and aseptic meningitis. Rotavirus is one of the most common agents mortality associated with infection can be very significant in developing countries. Most viruses produce that are not distinct other pathogens, current diagnostics limited breadth sensitivity required advance detection schemes for disease intervention strategies. A spectroscopic assay based on surface enhanced Raman...

Protein secondary structures may exhibit reversible transitions that occur in an abrupt and controllable manner. In this report, we demonstrate such be utilized the design of a "smart" protein micellar system, which stimulus-induced change structure triggers rapid micelle compacticity size. Specifically, recombinant DNA methods were used to prepare triblock copolymer containing central hydrophilic block two hydrophobic end blocks derived from elastin-mimetic peptide sequences. Below inverse...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteraction of dipalmitoylphosphatidylcholine and dimyristoylphosphatidylcholine-d54 mixtures with glycophorin. A Fourier transform infrared investigationRichard A. Dluhy, Richard Mendelsohn, Hector L. Casal, Henry H. MantschCite this: Biochemistry 1983, 22, 5, 1170–1177Publication Date (Print):March 1, 1983Publication History Published online1 May 2002Published inissue 1 March...

We have demonstrated label-free optical detection of viral nucleoprotein binding to a polyvalent anti-influenza aptamer by monitoring the surface-enhanced Raman (SERS) spectra aptamer-nucleoprotein complex. The SERS that selective complex could be differentiated from alone based solely on direct spectral signature for Multivariate statistical methods, including principal components analysis, hierarchical clustering, and partial least squares, were used confirm statistically significant...

The prokaryote Mycoplasma pneumoniae is a major cause of respiratory disease in humans, accounting for 20% all community-acquired pneumonia and the leading older children young adults. limitations existing options mycoplasma diagnosis highlight critical need new detection platform with high sensitivity, specificity, expediency. Here we evaluated silver nanorod arrays (NA) as biosensing differentiation M. culture spiked true clinical throat swab samples by surface-enhanced Raman spectroscopy...

The angular dependence of surface enhanced Raman scattering (SERS) has been investigated for molecules adsorbed onto Ag nanorod array substrates fabricated using an oblique angle deposition technique. strong SERS signal trans-1,2-bis(4-pyridyl) ethene (BPE) was found to be strongly dependent on the incident excitation laser, with maximum intensity appearing at approximately 45° relative normal. A model based classical electrodynamic dipole radiation developed BPE these substrates, and...

The interaction of a chemically synthesized 25-residue signal peptide LamB protein from Escherichia coli with phospholipids has been studied film balance technique. conformation, orientation, and concentration the peptides in lipid monolayers have determined polarized infrared spectroscopy, ultraviolet assay 14C-labeled transferred films. When is injected into subphase under phosphatidylethanolamine-phosphatidylglycerol monolayer at low initial pressure, insertion portion evidenced by rapid...