A5079861380- Spectroscopy and Chemometric Analyses
- Metabolomics and Mass Spectrometry Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Chemical Sensor Technologies
- Identification and Quantification in Food
- Microbial Metabolic Engineering and Bioproduction
- Advanced Proteomics Techniques and Applications
- Biosensors and Analytical Detection
- Meat and Animal Product Quality
- Listeria monocytogenes in Food Safety
- Gold and Silver Nanoparticles Synthesis and Applications
- Heat and Mass Transfer in Porous Media
- Bacterial Identification and Susceptibility Testing
- Bioinformatics and Genomic Networks
- Forensic Toxicology and Drug Analysis
- Lattice Boltzmann Simulation Studies
- Molecular Biology Techniques and Applications
- Aerosol Filtration and Electrostatic Precipitation
- Salmonella and Campylobacter epidemiology
- Computational Drug Discovery Methods
- Mass Spectrometry Techniques and Applications
- Melamine detection and toxicity
- Coconut Research and Applications
- Genomics and Phylogenetic Studies
- Analytical Chemistry and Chromatography
Avacta (United Kingdom)
2024
University of Manchester
2012-2021
Czech Academy of Sciences, Institute of Biotechnology
2015-2019
University of Wales
2001-2007
Aberystwyth University
2001-2005
Institut des Sciences Biologiques
2002
University of Bradford
1982-1995
Phenotyping of 1,200 'healthy' adults from the UK has been performed through investigation diverse classes hydrophilic and lipophilic metabolites present in serum by applying a series chromatography-mass spectrometry platforms. These data were made robust to instrumental drift numerical correction; this was prerequisite allow detection subtle metabolic differences. The variation observed metabolite relative concentrations between subjects ranged less than 5 % more 200 %. Variations could be...
Major food adulteration and contamination events occur with alarming regularity are known to be episodic, the question being not if but when another large-scale safety/integrity incident will occur.
ABSTRACT Fourier transform infrared (FT-IR) spectroscopy is a rapid, noninvasive technique with considerable potential for application in the food and related industries. We show here that this can be used directly on surface of to produce biochemically interpretable “fingerprints.” Spoilage meat result decomposition formation metabolites caused by growth enzymatic activity microorganisms. FT-IR was exploited measure biochemical changes within substrate, enhancing accelerating detection...
There has been considerable progress recently towards developing therapeutic strategies for Huntington's disease (HD), with several compounds showing beneficial effects in transgenic mouse models. However, human trials HD are difficult, costly and time-consuming due to the slow course, insidious onset patient-to-patient variability. Identification of molecular biomarkers associated progression will aid development effective therapies by allowing further validation animal models providing...
Missing values are known to be problematic for the analysis of gas chromatography-mass spectrometry (GC-MS) metabolomics data. Typically these cover about 10%-20% all data and can originate from various backgrounds, including analytical, computational, as well biological. Currently, most substitute missing is a mean imputation. In fact, some researchers consider this aspect in their pipeline so routine that they do not even mention using replacement approach. However, may have significant...
Adulteration of high quality food products with sub-standard and cheaper grades is a world-wide problem taxing the global economy. Currently, many traditional tests suffer from poor specificity, highly complex outputs lack high-throughput processing. Metabolomics has been successfully used as an accurate discriminatory technique in number applications including microbiology, cancer research environmental studies certain types fraud. In this study, we have developed metabolomics to assess...
The stability of mammalian serum and urine in large metabolomic investigations is essential for accurate, valid reproducible studies. urine, either processed immediately by freezing at -80 degrees C or stored 4 24 h before being frozen, was compared a pilot study samples from 40 separate healthy volunteers.Metabolic profiling with GC-TOF-MS performed collected volunteers frozen C. Subsequent Wilcoxon rank sum test Principal Components Analysis (PCA) methods were used to assess whether...
Summary Metabolomic approaches were used to elucidate some key metabolite changes occurring during interactions of Magnaporthe grisea – the cause rice blast disease with an alternate host, Brachypodium distachyon . Fourier‐transform infrared (FT‐IR) spectroscopy provided a high‐throughput metabolic fingerprint M. interacting B. accessions ABR1 (susceptible) and ABR5 (resistant). Principal component–discriminant function analysis (PC‐DFA) allowed differentiation between developing symptoms...
Abstract Major food adulteration incidents occur with alarming frequency and are episodic, the latest incident, involving of meat from 21 producers in Brazil supplied to 60 other countries, reinforcing this view. Food fraud counterfeiting involves all types foods, feed, beverages, packaging, potential for serious health, as well significant economic social impacts. In spirit drinks sector, counterfeiters often ‘recycle’ used genuine or employ good quality simulants. To prove that suspect...
The application of Raman spectroscopy as a detection method coupled with liquid chromatography (LC) has recently attracted considerable interest, although this currently been limited to isocratic elution. combination LC rapidly advancing techniques, such surface-enhanced scattering (SERS), allows for rapid separation, identification and quantification, leading quantitative discrimination closely eluting analytes. This study demonstrated the utility SERS in conjunction reversed-phase (RP-LC),...
Food fraud has been identified as an increasing problem on a global scale with wide-ranging economic, social, health and environmental impacts. Omics their related techniques, approaches, bioanalytical platforms incorporate significant number of scientific areas which have the potential to be applied significantly reduce food its negative In this overview we consider selected very recent studies where omics techniques were detect authenticity could implemented ensure integrity. We postulate...
There has been an increasing demand for rapid and sensitive techniques the identification quantification of pharmaceutical compounds in human biofluids during past few decades, surface-enhanced Raman scattering (SERS) is one a number physicochemical with potential to meet these demands. In this study we have developed SERS-based analytical approach assessment combination chemometrics. This novel enabled detection β-blocker propranolol spiked into serum, plasma, urine at physiologically...