A5011711981- Spectroscopy Techniques in Biomedical and Chemical Research
- Dental Implant Techniques and Outcomes
- Bone Tissue Engineering Materials
- Optical Imaging and Spectroscopy Techniques
- Veterinary Pharmacology and Anesthesia
- Protein Interaction Studies and Fluorescence Analysis
- Spectroscopy and Chemometric Analyses
- Lipid Membrane Structure and Behavior
- Bacillus and Francisella bacterial research
University of Nottingham
2019-2023
Using phantom samples, we investigated the feasibility of spatially-offset Raman spectroscopy (SORS) as a tool for monitoring non-invasively mineralization bone tissue engineering scaffold in-vivo. The samples consisted 3D-printed scaffolds poly-caprolactone (PCL) and hydroxyapatite (HA) blends, with varying concentrations HA, to mimic mineralisation process. were covered by 4 mm layer skin simulate real in-vivo measurement conditions. At concentration HA approximately 1/3 that (~0.6 g/cm3),...
A wide range of biomaterials and tissue-engineered scaffolds are being investigated to support stimulate bone healing in animal models. Using phantoms rat cadavers, we the feasibility using spatially offset Raman spectroscopy (SORS) monitor changes collagen concentration at levels similar those expected occur vivo during regeneration (0-0.84 g/cm3 ). partial least squares (PLS) regression model was developed quantify plugs consisting mixtures or hydroxyapatite (predictive power ±0.16 The PLS...
Understanding and quantifying the temporal acquisition of host cell molecules by intracellular pathogens is fundamentally important in biology. In this study, a recently developed holographic optical trapping (HOT)-based Raman microspectroscopy (RMS) instrument applied to detect, characterize monitor real time molecular trafficking specific species (isotope-labeled phenylalanine (L-Phe(D8)) at single level. This approach enables simultaneous measurement chemical composition human...
Spatially offset Raman spectroscopy (SORS) is a powerful technique for subsurface molecular analysis of optically turbid samples. Numerical modeling light propagation has been used to investigate opportunities improving spectral contrast and signal noise ratio when imaging regions interest located 0-4.5 mm below the surface in polymer bulk material. Two- three-dimensional results demonstrate that analyzing certain region (ROI) finite lateral dimensions sample surface, offsetting both laser...
By optimising instrumentation design using computational modelling of diffuse optics, we have developed a Raman instrument capable monitoring fibrotic tissue growth, first in phantoms, then cadavers, and finally progressing to in-vivo measurements.
Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modeling light propagation was used as a method for improving the design an DRS instrument to maximize signal noise ratio (SNR) while ensuring safe laser exposure parameters required in-vivo measurements. Experimental validation model performed on both phantom samples and disks implanted postmortem mimic typical response foreign bodies (formation fibrotic capsule around implant). A...