Abstract Preservation of natural tooth structure requires early detection the carious lesion and is associated with comprehensive patient dental care. Processes aiming to detect lesions in initial stage optimum efficiency employ a variety technologies such as magnifying loupes, transillumination, light laser fluorescence ( QLF ® DIAGNO dent ) autofluorescence (Soprolife VistaCam ), electric current/impedance (CarieScan tomographic imaging image processing. Most fluorescent caries tools can...

The goals of this trial were, first, to produce a Raman mapping decay and sound dentin samples, through accurate analysis the band spectra variations mineral organic components. second goal was confirm correlation between signal fluorescent camera, by assaying concentration pentosidine natural collagen crosslink using reverse phase high-pressure liquid chromatography. first assumed possible relationship observed with camera spectroscopy. an association Maillard reaction. Absence for that no...

In regenerative medicine, stem-cell-based therapy often requires a scaffold to deliver cells and/or growth factors the injured site. Porous silicon (pSi) is promising biomaterial for tissue engineering as it both nontoxic and bioresorbable. Moreover, surface modification can offer control over degradation rate of pSi also promote cell adhesion. Dental pulp stem (DPSC) are pluripotent mesenchymal found within teeth constitute readily source cells. Thus, coupling good proliferation...

The way cells explore their surrounding extracellular matrix (ECM) during development and migration is mediated by lamellipodia at leading edge, acting as an actual motor pulling the cell forward. Lamellipodia are primary area within of actin microfilaments (filopodia) formation. In this work, we report on use porous silicon (pSi) scaffolds to mimic ECM mesenchymal stem from dental pulp (DPSC) breast cancer (MCF-7) cells. Our atomic force microscopy (AFM), fluorescence microscopy, scanning...

Antibacterial surfaces have been in the focus of research for years, driven by an unmet clinical need to manage increasing incidence implant-associated infections. The use silver has become a topic interest because its proven broad-spectrum antibacterial activity and track record as coating agent soft tissue implants catheters. However, time being, translation these technological achievements improvement property hard titanium (Ti) remains unsolved. In our study, we focused on investigation...

The rheological properties of the periodontal ligament are key parameters to understand homeostatic stability tooth supporting apparatus. objective this study is lay new insights on and structural information different regions in murine by using atomic force (AFM) multi-photon microscopy (MPM). A significant variation elasticity was measured. showed a tendency become softer towards furcation, nearest area center resistance tooth. This can open prospective for connecting adaptation geometry...

Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on spectra cells components, and their monitored through diffusion cytochrome c in cytoplasm. K-mean clustering identify mitochondria cells, correlation analysis provides the distribution inside Our results demonstrate that incubation for 3 h with 10 μM paclitaxel does not induce On contrary, 30 min at higher concentration (100 μM) induces gradual release...

The European Union (EU) directive 2010/63/EU on the protection of animals used for scientific purpose focused reducing number and refinement breeding. Animal studies are necessary to protect human health. Lots animal models exist study bone regeneration, but a reliable, well reproducible, relatively inexpensive model with possibility multiple testing in same is still missing. Rats may serve as good this due small size cost/benefit ratio. present aimed develop novel rat caudal vertebrae...

Abstract Durch Reaktion zwischen Acrylnitril ( 1 ) und Alkalimetallpolybutadienylen wurden lösliche Agenzien vom Typ RCH 2 CH(CN)M hergestellt. An derartigen Li‐, Na‐ K‐Verbindungen 10 – 12 UV‐spektrometrische konduktometrische Bestimmungen im Temperaturbereich von –70 bis +25° ausgeführt sowie Dissoziationskonstanten, Dissoziationsenthalpien C–M‐Distanzen berechnet. Entsprechend diesen Ergebnissen treten die Verbindungen in Tetrahydrofuran als beständige Kontakt‐Ionenpaare auf.

Multiphoton microscopy has been used to reveal structural details of dentine and enamel at the dentin-enamel junction (DEJ) based on their 2-photon excited fluorescence (2PEF) emission second harmonic generation (SHG). In tubule 2PEF intensity varies due protein content variation. Intertubular dentin produces both SHG signals. Tubules are surrounded by a thin circular zone with lower signal than bulk presence collagen fibers perpendicular longitudinal axis is indicated strong responses. The...

Further development of biomaterials is expected as advanced therapeutic products must be compliant to good manufacturing practice regulations. A spraying method for building-up polyelectrolyte films followed by the deposition dental pulp cells presented. Physical treatments UV irradiation and a drying/wetting process are applied system. Structural changes elasticity modifications obtained coatings revealed atomic force microscopy Raman spectroscopy. This procedure results in thicker, rougher...

ABSTRACT Proper treatment of dental caries demands detection carious lesions at an early stage and a minimal invasive cavity preparation to preserve the maximum tooth structure. Various devices use fluorescence for via recording red generated by dentin under illumination. The aim this study was evaluate porphyrin pentosidine involvement in observed enamel when illuminated with Soprolife® camera (Sopro, Acteon Group, La Ciotat, France) Vistacam® (Dürr Dental AG, Bietigheim-Bissingen,...

Multiphoton confocal microscopy and nonlinear spectroscopy are used to investigate the caries process in dentin. Although dentin is a major calcified tissue of teeth, its organic phase comprises type I collagen fibers. Caries drive demineralization denaturation. powerful imaging technique: biological materials transparent infrared frequencies can be excited penetration depths inaccessible 1-photon microscopy. The laser excitation greatly reduces photodamage sole focal region, signal-to-noise...

Abstract Photobiomodulation (PBM) therapy is a continuously growing approach to stimulating healing and reducing inflammation pain. However, its effects in the fields of regenerative medicine tissue engineering are still under investigation. Studying PBM on capacity zebrafish can allow application novel clinical approaches where impact will be cross‐linked with stem‐cell therapeutic approaches. This study was done establish an in‐vivo experimental setup for studying laser ultraviolet...

All the alloplasmic common wheat lines having Agropyron trichophorum cytoplasm were found to carry one extra submetacentric or telocentric chromosome although they had been backcrossed seven times with as pollen parent. Behavior of and its interaction A, studied. was induce seed shriveling severe vigor reduction in wheat. The originating from carries a gene(s) for complete restoration plumpness normal plant vigor. Conscious selection plump seeds vigorous plants backcrossing has resulted...