A5032392442- Silicon Nanostructures and Photoluminescence
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Nanowire Synthesis and Applications
- Electrospun Nanofibers in Biomedical Applications
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Cellular Mechanics and Interactions
- Nanoparticle-Based Drug Delivery
- Graphene and Nanomaterials Applications
- Molecular Communication and Nanonetworks
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Water management and technologies
- Advanced Sensor and Energy Harvesting Materials
- Inhalation and Respiratory Drug Delivery
- Therapeutic Uses of Natural Elements
- Advanced Drug Delivery Systems
- Microfluidic and Capillary Electrophoresis Applications
- Polymer Surface Interaction Studies
- Click Chemistry and Applications
- Characterization and Applications of Magnetic Nanoparticles
- Pluripotent Stem Cells Research
Centre National de la Recherche Scientifique
2013-2025
Sorbonne Université
2010-2025
PHENIX laboratory
2014-2025
Physique des Cellules et Cancers
2024
Institut Curie
2024
Sorbonne University Abu Dhabi
2021
University of Florida
2014-2018
Institut Charles Gerhardt Montpellier
2010-2015
École Nationale Supérieure de Chimie de Montpellier
2010-2015
Gainesville Obstetrics & Gynecology
2015
Porous silicon nanoparticles (pSiNPs) act as a sensitizer for the 2-photon excitation of pendant porphyrin using NIR laser light, imaging and photodynamic therapy. Mannose-functionalized pSiNPs can be vectorized to MCF-7 human breast cancer cells through mannose receptor-mediated endocytosis mechanism provide 3-fold enhancement PDT effect.
Abstract We describe the preparation of biodegradable porous silicon nanoparticles (pSiNP) functionalized with cancer cell targeting antibodies and loaded hydrophobic anti‐cancer drug camptothecin. Orientated immobilization antibody on pSiNP is achieved using novel semicarbazide based bioconjugate chemistry. To demonstrate generality this approach, three MLR2, mAb528 Rituximab are used, which target neuroblastoma, glioblastoma B lymphoma cells, respectively. Successful demonstrated by means...
Poly(ethylene glycol) based hydrogel microparticles were developed for pulmonary drug delivery. Hydrogels are particularly attractive delivery because they can be size engineered into the bronchi, yet also swell upon reaching their destination to avoid uptake and clearance by alveolar macrophages. To develop enzyme-responsive a new synthesis method on solution polymerization was developed. This produces spherical poly(ethylene (PEG) from high molecular weight diacrylate (PEGDA)-based...
In regenerative medicine, stem-cell-based therapy often requires a scaffold to deliver cells and/or growth factors the injured site. Porous silicon (pSi) is promising biomaterial for tissue engineering as it both nontoxic and bioresorbable. Moreover, surface modification can offer control over degradation rate of pSi also promote cell adhesion. Dental pulp stem (DPSC) are pluripotent mesenchymal found within teeth constitute readily source cells. Thus, coupling good proliferation...
Non-toxic porous silicon nanoparticles carry porphyrin covalently attached to their surface inside breast cancer cells for a more efficient photodynamic effect.
A chitosan/porous silicon biosensing platform for the detection of carboxylic acid-containing drugs in water is prepared and characterized. Parasitic layer-free films mesoporous are electrochemically etched functionalized by covalent attachment chitosan oligomers. The presence species covering inner outer surface porous confirmed infrared spectroscopy nitrogen adsorption analysis. obtained hybrid offers both an important porosity, with a higher area than original substrate, charge, very...
The way cells explore their surrounding extracellular matrix (ECM) during development and migration is mediated by lamellipodia at leading edge, acting as an actual motor pulling the cell forward. Lamellipodia are primary area within of actin microfilaments (filopodia) formation. In this work, we report on use porous silicon (pSi) scaffolds to mimic ECM mesenchymal stem from dental pulp (DPSC) breast cancer (MCF-7) cells. Our atomic force microscopy (AFM), fluorescence microscopy, scanning...
Abstract The specific targeting of intracellular proteins or organelles by magnetic nanoparticles (MNPs) is a major challenge in nanomedicine, as most MNPs are internalized cells through endocytosis and remain trapped inside small vesicles, limiting their ability to reach components. Furthermore, this phenomenon limits heating capacity hyperthermia, therefore potential for cancer treatment. This study presents strategy based on an original double functionalization MNPs, with polyhistidine...
Hydrogel microparticles are particularly attractive for pulmonary drug delivery. Their size can be engineered efficient delivery into the bronchi, where they subsequently swell, avoiding macrophage uptake. In this study, enzyme-responsive peptide functionalized poly(ethylene glycol) (PEG) based hydrogel were synthesized by an emulsion polymerization. Here, we demonstrate that these nontoxic and demonstrated their viability as a carrier studying encapsulation release of three types drugs:...
Parkinson's disease (PD) is a neurodegenerative associated with loss or dysfunction of dopaminergic neurons located in the substantia nigra (SN), and there no cure available. An emerging new approach for treatment to transplant human induced directly into denervated striatal brain target region. Unfortunately, grafted are unable grow axons striatum thus do not allow recovery original connectivity. Towards overcoming this general limitation guided neuronal regeneration, we develop here...
The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, use magnetic nanoparticles appears to be promising, since fields can act at distance without interactions with surrounding biological system. To control subcellular spatial resolution, used either induce biochemical reactions locally apply forces on elements cell. Here, we show cell...
The mechanical manipulation of magnetic nanoparticles is a powerful approach to probing and actuating biological processes in living systems. Implementing this technique high-throughput assays can be achieved using biocompatible micromagnet arrays. However, the properties these arrays are usually indirectly inferred from simulations or Stokes drag measurements, leaving unresolved questions about actual profile fields at micrometer scale exact forces that applied. Here, we exploit field...
The endosomal entrapment of functional nanoparticles is a severe limitation to their use for biomedical applications. In the case magnetic (MNPs), this leads poor heating efficiency hyperthermia and suppresses possibility manipulate them in cytosol. Current strategies limit include functionalization with cell-penetrating peptides promote translocation directly across cell membrane or facilitate escape. However, these suffer from potential release free cell, best our knowledge, there...
A challenge in current stem cell therapies for Parkinson's disease (PD) is controlling neuronal outgrowth from the substantia nigra towards targeted area where connectivity required striatum. Here we present progress directional neurite extensions through application of iron-oxide magnetic nanoparticles (MNPs) labelled cells combined with a array generating large spatially variant field gradients (greater than 20 T m −1 ). We investigated viability this approach both two-dimensional and...
Photoluminescent silicon nanocrystals are very attractive for biomedical and electronic applications. Here a new process is presented to synthesize photoluminescent with diameters smaller than 6 nm from porous template. These nanoparticles formed using pore-wall thinning approach, where the as-etched layer partially oxidized silica, which dissolved by hydrofluoric acid solution, decreasing thickness. This decrease in thickness leads corresponding size of that make up pore walls, resulting...
Abstract The axon regeneration of neurons in the brain can be enhanced by activating intracellular signaling pathways such as those triggered membrane-anchored Rat sarcoma (RAS) proto-oncogene. Here we demonstrate induction neurite growth expressing tagged permanently active Harvey-RAS protein or RAS-activating catalytic domain guanine nucleotide exchange factor (SOS1cat), secondary dopaminergic cells. Due to tag, expressed fusion is captured functionalized magnetic nanoparticles cytoplasm...
Abstract Remote control of cellular functions via magnetic forces offers unique opportunities in fundamental research and biomedical application. Intracellular delivery functionalized nanoparticles (MNP) provides versatile to assemble signalling platforms for spatiotemporal by forces. Such magnetogenetic application, however, has remained highly challenging due a lack MNP providing suitable biological, physicochemical properties. Here, we achieved single-step surface coating synthetic...
Endocytic recycling pathways play key roles in the re-routing of cargoes through cell to control a broad range cellular processes, and many vesicle trafficking regulators are implicated progression disease such as cancer. The Rab11 family (Rab11a, Rab11b, Rab25) return internalised plasma membrane, Rab25 has been aggressiveness cancer by promoting invasive migration. However, whilst vesicles distribute leading edge moving cells, how directly they contribute protrusion is not clear. Here we...