A5002757172- Wnt/β-catenin signaling in development and cancer
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Connective tissue disorders research
- Nanoparticle-Based Drug Delivery
- Tendon Structure and Treatment
- Periodontal Regeneration and Treatments
- Spaceflight effects on biology
- Ion channel regulation and function
- Microtubule and mitosis dynamics
- Microfluidic and Bio-sensing Technologies
- Erythrocyte Function and Pathophysiology
- Bone fractures and treatments
- 3D Printing in Biomedical Research
- Characterization and Applications of Magnetic Nanoparticles
- Neuroscience and Neural Engineering
- Muscle Physiology and Disorders
- Bone Tissue Engineering Materials
- Osteoarthritis Treatment and Mechanisms
- Micro and Nano Robotics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Surface Treatment and Residual Stress
- Mesenchymal stem cell research
- Autism Spectrum Disorder Research
- Advanced Materials and Mechanics
Keele University
2013-2025
University of Birmingham
2022-2025
NIHR Birmingham Biomedical Research Centre
2024
Regenerative medicine is a pioneering field aimed at restoring and regenerating the function of damaged cells, organs tissues in order to establish normal function. It demands cross communication disciplines develop effective therapeutic stem cell based therapies. Nanotechnology has been instrumental development translation basic research clinically relevant In particular, magnetic nanoparticles (MNPs) have applied tag, track activate cells offering an means monitoring vitro vivo behaviour....
Significance Efficient delivery of therapeutic molecules inside cells by nontransgenic approaches is key as gene editing/correction, directed differentiation, and in vivo cell modulation/tracking are translated for regenerative medicine applications. Here we describe a peptide-based system engineered to enhance the activity cell-penetrating peptides achieve exceptional intracellular transduction. Glycosaminoglycan-binding enhanced transduction (GET) uses that interact with membrane heparan...
Abstract Bone requires dynamic mechanical stimulation to form and maintain functional tissue, yet stimuli are often lacking in many therapeutic approaches for bone regeneration. Magnetic nanoparticles provide a method delivering these by directly targeting cell-surface mechanosensors transducing forces from an external magnetic field, resulting remotely controllable mechanotransduction. In this investigation, functionalized were attached either the mechanically gated TREK1 K+ channel or...
Wnt signalling pathways play crucial roles in developmental biology, stem cell fate and tissue patterning have become an attractive therapeutic target the fields of engineering regenerative medicine. has also been shown to a role human Mesenchymal Stem Cell (hMSC) fate, which potential as therapy bone cartilage engineering. Previous work that biocompatible magnetic nanoparticles (MNP) can be used stimulate specific mechanosensitive membrane receptors ion channels vitro vivo. Using this...
Magnetic ion channel activation technology uses superparamagnetic nanoparticles conjugated with targeting antibodies to apply mechanical force directly stretch-activated channels on the cell surface, stimulating mechanotransduction and downstream processes. This technique has been reported promote differentiation towards musculoskeletal types enhance mineralisation. Previous studies have shown how mesenchymal stem cells injected into a pre-mineralised environment such as foetal chick...
Bone tissue engineering requires a combination of materials, cells, growth factors and mechanical cues to recapitulate bone formation. In this study we evaluated hybrid hydrogels for minimally invasive formation by combining biomaterials with skeletal stem cells staged release together mechanotransduction. Hybrid consisting alginate decellularized, demineralised extracellular matrix (ALG/ECM) were seeded Stro-1+ human marrow stromal (HBMSCs). Dual combinations within staged-release...
The timing, location, and level of WNT signaling are highly regulated during embryonic development for the maintenance adult tissues. Consequently ability to provide a defined directed source proteins is crucial fully understand its role in tissue mimic activity vitro. Here we describe one-step immobilization technique covalently bind WNT3A as basal surface with easy storage long-lasting activity. We show that this platform able maintain stem cells while also being adaptable 3D systems....
TWIK-related K+ 1 (TREK1) is a potassium channel expressed in the nervous system with multiple functions including neurotransmission and prime pharmacological target for neurological disorders. TREK1 gating controlled by wide range of external stimuli mechanical forces. Previous work has demonstrated that can be mechano-activated using magnetic nanoparticles (MNP) functionalised antibodies targeted to channels. Once MNP are bound, dynamic fields used generate forces on TREK channel. This...
Abstract Wnt signaling is a key developmental pathway that regulates dopaminergic progenitor cell proliferation and differentiation during neuronal development. This makes an important therapeutic target for neurodegenerative conditions such as Parkinson's disease. can be modulated using peptides UM206, which bind to the receptor Frizzled. Previous work has demonstrated remote activation of through Frizzled peptide‐functionalized magnetic nanoparticles (MNPs) with field stimulation. Using...
A challenge in current stem cell therapies for Parkinson's disease (PD) is controlling neuronal outgrowth from the substantia nigra towards targeted area where connectivity required striatum. Here we present progress directional neurite extensions through application of iron-oxide magnetic nanoparticles (MNPs) labelled cells combined with a array generating large spatially variant field gradients (greater than 20 T m −1 ). We investigated viability this approach both two-dimensional and...
Bone regeneration and repair are complex processes in the adult skeleton, current research has focused on understanding controlling these processes. Magnetic nanoparticle (MNP)-based platforms have shown potential tissue engineering regenerative medicine through use of magnetic nanomaterials combined with remotely applied dynamic fields. Previous studies demonstrated ability MNP-induced mechanoactivation to trigger downstream signaling promote new bone formation. In this study, we aimed...
The study of exogenous and endogenous nanoscale magnetic material in biology is important for developing biomedical nanotechnology as well understanding fundamental biological processes such iron metabolism biomineralization. Here, we exploit the magneto-optical Faraday effect to probe intracellular properties perform imaging, revealing location-specific magnetization dynamics nanoparticles within cells. opportunities enabled by this method are shown context hyperthermia; an where local...
Wnt signaling plays an important role in embryogenesis and adult stem cell homeostasis. Its diminished activation is implicated osteoporosis degenerative neural diseases. However, systematic administration of Wnt-signaling agonists carries risk, as aberrantly activated Wnt/β-catenin linked to cancer. Therefore, technologies for local modulation control targeted specific sites disease or degeneration have potential therapeutic value the treatment We reported a facile approach locally activate...
This study reports results of a mechanical platform-based screening assay (MICA) to evaluate the remote activation mechanosensitive ion channels. Here, we studied ERK pathway and elevation in intracellular Ca2+ levels response MICA application using Luciferase Fluo-8AM assay, respectively. Functionalised magnetic nanoparticles (MNPs) targeting membrane-bound integrins TREK1 channels were with HEK293 cell lines under application. The demonstrated that active via RGD (Arginylglycylaspartic...
Growth factors play a crucial role in regulating various cellular functions, including proliferation and differentiation. Consequently, the biomaterial-based delivery of exogenous growth presents promising strategy regenerative medicine to manage healing process restore tissue function. For effective therapeutic applications, it is essential that these active compounds are precisely targeted site regeneration, with release kinetics align slow pace growth. We have developed an ex vivo model...
In the biomedical field, there is a demand for development of novel approaches investigation optical epithelial anatomical features with biomimetic materials. These materials are not only required to replicate structures but also enable dynamic modelling disease states such as limbal stem cell deficiency and ageing. present study, effective generation reversible wrinkled polydimethylsiloxane (PDMS) substrates was undertaken mimic undulating anatomy niche. This surface pattern formed through...