A5079889946- Monoclonal and Polyclonal Antibodies Research
- Radiopharmaceutical Chemistry and Applications
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Enzyme Production and Characterization
- Bone Tissue Engineering Materials
- Advanced Biosensing Techniques and Applications
- Synthesis and Biological Evaluation
- Enzyme Catalysis and Immobilization
- Neuroscience and Neuropharmacology Research
- CAR-T cell therapy research
- Protein Kinase Regulation and GTPase Signaling
- Cellular Mechanics and Interactions
- Peptidase Inhibition and Analysis
- PI3K/AKT/mTOR signaling in cancer
- Nanofabrication and Lithography Techniques
- Nanoplatforms for cancer theranostics
- Microfluidic and Bio-sensing Technologies
- Thyroid Disorders and Treatments
- Protein purification and stability
- Diatoms and Algae Research
- Peroxisome Proliferator-Activated Receptors
- Oral and gingival health research
- DNA and Nucleic Acid Chemistry
- Silk-based biomaterials and applications
Ruhr University Bochum
2008-2024
Philipps University of Marburg
2022
Roche (Switzerland)
2012-2020
Goethe University Frankfurt
2019-2020
APC Microbiome Institute
2020
Cardiff University
2020
Friedrich-Loeffler-Institut
2014
University Medical Center of the Johannes Gutenberg University Mainz
2013
Johannes Gutenberg University Mainz
2007-2013
CS Diagnostics
2013
Calcium phosphate has excellent biocompatibility due to its chemical similarity human hard tissue (bone and teeth). In nanoparticulate dispersed form, it can be used as a carrier in biological systems, e.g. transfer nucleic acids or drugs. If such nanoparticles are suitably functionalized with fluorescing dyes, they also for imaging photodynamic therapy.
CEA TCB is a novel IgG-based T-cell bispecific (TCB) antibody for the treatment of CEA-expressing solid tumors currently in phase I clinical trials (NCT02324257). Its format incorporates bivalent binding to CEA, head-to-tail fusion CEA- and CD3e-binding Fab domains an engineered Fc region with completely abolished FcγRs C1q. The study provides mechanistic insights into activity mode action TCB.CEA was characterized on 110 cell lines vitro xenograft tumor models vivo using NOG mice engrafted...
A new rhenium tricarbonyl complex of a bis(quinoline)-derived ligand (2-azido-N,N-bis((quinolin-2-yl)methyl)ethanamine, L–N3), namely [Re(CO)3(L–N3)]Br was synthesized and characterized in-depth, including by X-ray crystallography. exhibits strong UV absorbance in the range 300–400 nm with maximum at 322 nm, upon photoexcitation, shows two distinct emission bands about 430 560 various solvents (water, ethylene glycol). could be conjugated, on solid phase, to peptide nucleic acid (PNA)...
Pursuing our aim to develop a biomimetic synthetic scaffold suitable for tissue engineering, we embedded bone cells, osteoblast-related SaOS-2 cells and osteoclast-like RAW 264.7 into beads, formed of Na-alginate-based or silica-containing hydrogel matrix. The beads were incubated either separately (only one cell line in culture dish) co-incubated (SaOS-2-containing beads). alginate alginate/silica matrices found not impair the viability encapsulated cells. In these retain their capacity...
Abstract Two peptide nucleic acid (PNA) oligomers containing two different organometallic moieties, namely derivatives of azidoferrocene (Fc‐N 3 ) and ferrocene carboxylic (Fc‐COOH) for PNA5 β‐cymantrenoylpropionic [Cym‐CO(CH 2 COOH] the rhenium bisquinoline tricarbonyl complex [Re(BQ‐N )(CO) ]Br PNA6 , have been prepared on a solid support by using synthetic methods: Peptide coupling click chemistry. were unambiguously characterized ESI‐MS or MALDI‐TOF MS their purity checked LC–MS. As...
Calcium phosphate nanoparticles were prepared by precipitation and colloidally stabilized surface functionalization with 5,10,15,20-tetrakis(4-phosphonooxyphenyl)porphine (p-TPPP). No other stabilizing agents necessary, all dissolved counter-ions excess porphyrin removed ultracentrifugation. The resulting porphyrin-functionalized spherical a diameter of about 250 nm. adsorption on the calcium was confirmed IR spectroscopy quantified elemental analysis (the loading 4–5 wt% porphyrin). In cell...
We previously showed that the serum- and glucocorticoid-inducible kinase 3 (SGK3) increases AMPA-type glutamate receptor GluA1 protein in plasma membrane. The activation of AMPA receptors by NMDA-type eventually leads to postsynaptic neuronal plasticity. Here, we show SGK3 mRNA is upregulated hippocampus new-born wild type Wistar rats after NMDA activation. further demonstrate Xenopus oocyte expression system delivery membrane depends on small GTPase RAB11. This RAB-dependent trafficking...
At present the scaffolds used for bioprinting of cells do not elicit morphogenetic responses in cells. In study we approached a solution by studying effect an inorganic silica supplement added to Na-alginate matrix. Bone- and osteoblast-like SaOS-2 were embedded into this organic polymeric matrix which was additionally enriched with 400 μM prehydrolyzed TEOS [tetra-ethoxy-silane], source ortho-silicate. silica-based synthesized hydroxyapatite crystallites after exposure mineralization...
Membrane envelopment and budding of negative strand RNA viruses (NSVs) is mainly driven by viral matrix proteins (M). In addition, several M are also known to be involved in host cell manipulation. Knowledge about the cellular targets detailed molecular mechanisms, however, poor for many proteins. For instance, Nipah Virus (NiV) protein trafficking through nucleus essential virus release, but nuclear NiV remain unknown. To identify interactors henipavirus proteins, tagged Hendra (HeV) were...
The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, use magnetic nanoparticles appears to be promising, since fields can act at distance without interactions with surrounding biological system. To control subcellular spatial resolution, used either induce biochemical reactions locally apply forces on elements cell. Here, we show cell...
Nonalcoholic steatohepatitis (NASH) is considered as severe hepatic manifestation of the metabolic syndrome and has alarming global prevalence. The ligand-activated transcription factors farnesoid X receptor (FXR) peroxisome proliferator-activated (PPAR) δ have been validated molecular targets to counter NASH. To achieve robust therapeutic efficacy in this multifactorial pathology, combined peripheral PPARδ-mediated activity effects FXR activation appear a promising multitarget approach. We...
Abstract Mutations in the gene coding for multi-domain protein leucine-rich repeat kinase 2 (LRRK2) are leading cause of genetically inherited Parkinson’s disease (PD). Two common found mutations R1441C and G2019S. In this study we identified phosphatase 2A (PP2A) as an interacting partner LRRK2. We were able to demonstrate that Ras complex (ROC) domain is sufficient interact with three subunits PP2A human neuroblastoma SH-SY5Y cells HeLa cells. The alpha subunit LRRK2 perinuclear region...
Abstract The axon regeneration of neurons in the brain can be enhanced by activating intracellular signaling pathways such as those triggered membrane-anchored Rat sarcoma (RAS) proto-oncogene. Here we demonstrate induction neurite growth expressing tagged permanently active Harvey-RAS protein or RAS-activating catalytic domain guanine nucleotide exchange factor (SOS1cat), secondary dopaminergic cells. Due to tag, expressed fusion is captured functionalized magnetic nanoparticles cytoplasm...
Calcium phosphate nanorods which are typically used as paste for bone substitution were functionalized by DNA or siRNA. The structure and morphology of the did not change functionalization indicated dynamic light scattering (DLS), scanning electron microscopy (SEM), high-resolution transmission (HRTEM). With a load nucleic acids about 2.7 wt%, transfection with HeLa T24 cells, gene silencing HeLa-EGFP cells. Removal water filtration gave an injectable content 2 43 wt%. This leads to...
The transfer of genetic information into living cells is a powerful tool to manipulate their protein expression by the regulation synthesis. This can be used for treatment genetically caused diseases (gene therapy). However, systemic application genes associated with number problems, such as targeted gene delivery and potential side effects. Here we present method spatial nanoparticle-based therapy. Titanium was electrophoretically coated DNA-functionalized calcium phosphate nanoparticles....
The orphan transcription factor nuclear receptor-related 1 protein (Nurr1, also known as NR4A2) plays a key role in embryonic development and maintenance of mesencephalic dopaminergic neurons the substantia nigra. Nurr1 deficiency is associated with Parkinson's disease where degenerate suggesting that counter-regulation activity may have therapeutic effects. Here, we bacterially expressed isolated human fusion containing N-terminal cell delivery domain derived from detoxified anthrax lethal...
Vertebrate epithelial cells typically express a specific set of keratins. In teleosts, keratins are also present in variety mesenchymal cells, which usually vimentin. Significantly, our previous studies revealed that virtually all known teleost evolved independently from those terrestrial vertebrates. To further elucidate the evolutionary scenario led to large and their complex expression patterns day we have investigated presence bichir, sturgeon gar.We discovered novel group type I with...
Ion-exchange chromatography is used in biopharmaceutical downstream processes to reduce product-related impurity levels. Because protein aggregate levels can be considered as a critical quality attribute, the removal of aggregated species primary importance. The addition polyethylene glycol (PEG) mobile phase ion-exchange was found significantly improve chromatographic separation monomers from aggregates. In this work, linear gradient elution experiments with monomeric and samples monoclonal...