A5062142726- Axon Guidance and Neuronal Signaling
- Connexins and lens biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Amyotrophic Lateral Sclerosis Research
- Corneal Surgery and Treatments
- Angiogenesis and VEGF in Cancer
- Neurogenetic and Muscular Disorders Research
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Corneal surgery and disorders
- Heat shock proteins research
- Cancer, Hypoxia, and Metabolism
- Neurological diseases and metabolism
- Traumatic Brain Injury and Neurovascular Disturbances
- Mitochondrial Function and Pathology
- Glioma Diagnosis and Treatment
- Circular RNAs in diseases
- Retinal Diseases and Treatments
Ruhr University Bochum
2016-2025
Creative Commons
2022
Theiss Research
2015-2022
University of Würzburg
2019
Aarhus University
2016
University of Bonn
2003
University of Florida
1986
We have established a procedure for isolating native peroxisomal membrane protein complexes from cultured human cells. Protein-A-tagged peroxin 14 (PEX14), central component of the translocation machinery was genomically expressed in Flp-In-293 cells and purified digitonin-solubilized membranes. Size-exclusion chromatography revealed existence distinct multimeric PEX14 assemblies at membrane. Using mass spectrometric analysis, almost all known peroxins involved import were identified as...
Remote ischemic preconditioning (RIPC) can evoke cardioprotection following ischemia/reperfusion and this may depend on the anesthetic used. We tested whether 1) extracellular vesicles (EVs) isolated from humans undergoing RIPC protect cardiomyoblasts against hypoxia-induced apoptosis 2) effect is altered by cardiomyoblast exposure to isoflurane or propofol. EVs were before 60 min after Sham ten patients coronary artery bypass graft surgery with anesthesia quantified Nanoparticle Tracking...
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by motor neuron degeneration in the central nervous system. Recent research has increasingly linked activation of nucleotide oligomerization domain-like receptor protein 3 (NLRP3) inflammasome to ALS pathogenesis. NLRP3 triggers Caspase 1 (CASP 1) auto-activation, leading cleavage Gasdermin D (GSDMD) and pore formation on cellular membrane. This process facilitates cytokine secretion ultimately results...
The effect of ethanol in vitro on inositol lipid metabolism brain slices was investigated under nonstimulating and stimulating conditions. In cerebral cortical 100 microM norepinephrie (NE), 1 mM carbachol, serotonin, 20 KCl, glutamate 30 A23187 stimulated inositide hydrolysis as measured by the release [3H]inositol phosphates from [3H]myoinositol labeled slices. Ethanol (500 mM) inhibited nonstimulated but had variable effects breakdown. NE-, KCl- glutamate-stimulated phosphate 500 cortex....
The purpose of the present study was to investigate effects vascular endothelial growth factor (VEGF) on gap junctional intercellular communication (GJIC), cell proliferation, and dynamics in primary astrocytes. VEGF is known as a dimeric polypeptide that potentially binds two receptors, VEGFR-1 VEGFR-2, however many are mediated by for example, actin polymerization, forced migration, angiogenesis, proliferation. Recently it has been shown case hypoxia, ischemia or injury upregulated...
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder of the upper and lower motor neurons, characterized by rapid progressive weakness, muscle atrophy, dysarthria, dysphagia, dyspnea. Whereas exact cause ALS remains uncertain, wobbler mouse (phenotype WR; genotype wr/wr) equally develops degeneration neurons in spinal cord cortex with striking similarities to sporadic human ALS, suggesting possibility common pathway cell death.
Retinal ischemia is common in eye disorders, like diabetic retinopathy or retinal vascular occlusion. The goal of this study was to evaluate the potential protective effects an intravitreally injected endothelial growth factor (VEGF) inhibitor (ranibizumab) on cells animal model via immunohistochemistry (IF) and quantitative real-time PCR (PCR). A positive binding ranibizumab rat VEGF-A confirmed dot blot. One underwent a subgroup received ranibizumab. significant VEGF increase detected...
Amyotrophic lateral sclerosis (ALS) is the most common degenerative motor neuron disease in humans. However, pathogenesis of ALS not yet understood. The wobbler mouse considered as an animal model for sporadic form due to its spontaneous mutation Vps54 gene. Due transactivation NDRG2 by p53, this tumor suppressor might play a functional role stress induced cell death mice well patients. Furthermore, deregulated microRNAs are often related neurodegenerative diseases. Thus, linked miR-375-3p...
Cancer bioenergetics fuel processes necessary to maintain viability and growth under stress conditions. We hypothesized that cancer metabolism supports the repair of radiation-induced DNA double-stranded breaks (DSBs). combined systematic collection metabolic radiobiological data from a panel irradiated cell lines with mathematical modeling identified common response impact on DSB kinetics, including mitochondrial shutdown followed by compensatory glycolysis resumption function. Combining...
We previously showed that the serum- and glucocorticoid-inducible kinase 3 (SGK3) increases AMPA-type glutamate receptor GluA1 protein in plasma membrane. The activation of AMPA receptors by NMDA-type eventually leads to postsynaptic neuronal plasticity. Here, we show SGK3 mRNA is upregulated hippocampus new-born wild type Wistar rats after NMDA activation. further demonstrate Xenopus oocyte expression system delivery membrane depends on small GTPase RAB11. This RAB-dependent trafficking...
FE65 is a cytosolic adapter protein and an important binding partner of amyloid precursor protein. Dependent on Thr668 phosphorylation in protein, which influences amyloidogenic processing, undergoes nuclear translocation, thereby transmitting signal from the cell membrane to nucleus. As this translocation may be relevant Alzheimer disease, as consists three protein-protein interaction domains able bind affect variety other proteins downstream signaling pathways, identification interactome...
ABSTRACT Von Economo neurons (VENs) are large bipolar projection mainly located in layer Vb of anterior cingulate cortex (ACC) and insula. Both regions involved cognitive emotional procedures functionally anatomically altered schizophrenia. Although the detailed function VEN remains unclear, it has been suggested that these pathomechanism Here, we were interested question whether or not schizophrenia patients would show abnormalities at ultrastructural level. Accordingly, examined amount...