Retinal ischemia is a common pathomechanism in many ocular disorders such as age-related macular degeneration (AMD), diabetic retinopathy, glaucoma or retinal vascular occlusion. Several studies demonstrated that ischemia/reperfusion (I/R) leads to morphological and functional changes of different cell types. However, little known about the ischemic effects on optic nerve. The goal this study was evaluate these effects. Ischemia induced by raising intraocular pressure (IOP) one eye rats 140...

Glaucoma is a multifactorial disease and especially mechanisms occurring independently from an elevated intraocular pressure (IOP) are still unknown. Likely, the immune system contributes to glaucoma pathogenesis. Previously, IgG antibody depositions retinal ganglion cell (RGC) loss were found in IOP-independent autoimmune model. Therefore, we investigated possible participation of complement this Here, rats immunized with bovine optic nerve homogenate antigen (ONA), while controls (Co)...

Fast-spiking parvalbumin interneurons are critical for the function of mature cortical inhibitory circuits. Most these neurons enwrapped by a specialized extracellular matrix (ECM) structure called perineuronal net (PNN), which can regulate their synaptic input. In this study, we investigated relationship between PNNs, interneurons, and distribution on cells in adult primary visual cortex (V1) quadruple knockout mice deficient ECM molecules brevican, neurocan, tenascin-C, tenascin-R. We used...

Introduction Spinal cord injury involves complex pathobiological mechanisms, necessitating a multidimensional approach for its cure. Previous studies have shown that α9-integrin expression and activation in mature dorsal root ganglion neurons enable the regeneration of injured axons within spinal cord. However, tissue cavitation fibrosis impede regenerating from following their usual pathways, forcing them to seek alternative routes rich tenascin-C, primary ligand integrin. Fibrin gel, an...

To identify sensitivity and specificity of computerised cardiotocography (CTG) analysis for fetal acidosis during delivery.Retrospective observational study.Tertiary referral labour ward, Technical University München (TUM) Witten/Herdecke (UWH).All deliveries, which had at least one scalp pH measurement electronically saved CTG traces, between 2000 2002 2004 2005 (UWH).Correlation values International Federation Obstetrics Gynecology (FIGO) classification using 'CTG Online' program digitally...

Abstract Retinal ischemia occurs in a variety of eye diseases. Restrained blood flow induces retinal damage, which leads to progressive optic nerve degeneration and vision loss. Previous studies indicate that extracellular matrix (ECM) constituents play an important role complex tissues, such as retina nerve. They have great impact on de- regeneration processes represent major candidates central nervous system glial scar formation. Nevertheless, the importance ECM during ischemic...

Abstract In glaucoma, latest studies revealed an involvement of the complement system with and without elevated intraocular pressure. experimental autoimmune glaucoma model, immunization antigens, such as S100B, lead to retinal ganglion cell (RGC) loss optic nerve degeneration after 28 days. Here, we investigated timeline progression system, toll-like-receptor 4 (TLR4), transcription factor nucleus factor-kappa B (NFκB). Therefore, rats were immunized S100B protein (S100) analyzed at 3, 7,...

Abstract Glaucoma is characterized by the loss of retinal ganglion cells ( RGC s) and optic nerve fibres. Previous studies noted fewer s after immunization with ocular antigens at 28 days. It known that changes in extracellular matrix ECM ) components conduct retina degeneration. Here, we focused on remodelling tenascin‐C phosphacan/receptor protein tyrosine phosphatase β/ζ an autoimmune glaucoma model. Rats were immunized homogenate ONA or S100B (S100). Controls received sodium chloride...

<i>Objective:</i> To evaluate and compare the computer analyzing software system with subjective interpretation using FIGO classification of intrapartum cardiotocograms. <i>Methods:</i> Twenty-four obstetricians 19 midwives from 3 hospitals [19 junior (≤3 years) 24 senior (>3 experience] participated in this study. Forty-three doctors midwifes interpreted cardiotocographic (CTG) readings 12 parturients without knowing clinical outcome. Two CTG were repeated for...

Retinal ischemia is common in eye disorders, like diabetic retinopathy or retinal vascular occlusion. The goal of this study was to evaluate the potential protective effects an intravitreally injected endothelial growth factor (VEGF) inhibitor (ranibizumab) on cells animal model via immunohistochemistry (IF) and quantitative real-time PCR (PCR). A positive binding ranibizumab rat VEGF-A confirmed dot blot. One underwent a subgroup received ranibizumab. significant VEGF increase detected...

Cellular responses in glia play a key role regulating brain remodeling post-stroke. However, excessive glial reactivity impedes post-ischemic neuroplasticity and hampers neurological recovery. While damage-associated molecular patterns activated microglia were shown to induce astrogliosis, the molecules that restrain astrogliosis are largely unknown. We explored of tenascin-C (TnC), an extracellular matrix component involved wound healing injured tissues, mice exposed ischemic stroke induced...

Previous studies demonstrated that retinal damage correlates with a massive remodeling of extracellular matrix (ECM) molecules and reactive gliosis. However, the functional significance ECM in neurodegeneration is still unknown. In present study, we used an intraocular pressure (IOP) independent experimental autoimmune glaucoma (EAG) mouse model to examine role glycoprotein tenascin-C (Tnc). Wild type (WT ONA) Tnc knockout (KO mice were immunized optic nerve antigen (ONA) homogenate control...

The aim of this study was to determine the quality intrapartum uterine activity (UA) monitoring in daily practice during first and second stages labour. total duration inadequate UA is quantified relation technique applied, namely, external tocodynamometry (TOCO) or electrohysterography (EHG). 144 recordings, collected from 1^st September 2008 until 15^th October 2009 deliveries at Marien-Hospital Witten, Germany, were analysed by obstetricians based different centres....

Glaucoma is a neurodegenerative disease that characterized by the loss of retinal ganglion cells (RGC) and optic nerve fibers. Increased age intraocular pressure (IOP) elevation are main risk factors for developing glaucoma. Mice heterozygous (HET) mega-karyocyte protein tyrosine phosphatase 2 (PTP-Meg2) show chronic progressive IOP elevation, severe RGCs loss, damage, represent valuable model IOP-dependent primary open-angle glaucoma (POAG). Previously, evidence accumulated suggesting...

Visual processing depends on sensitive and balanced synaptic neurotransmission. Extracellular matrix proteins in the environment of cells are key modulators synaptogenesis plasticity. In present study, we provide evidence that combined loss four extracellular components, brevican, neurocan, tenascin-C, tenascin-R, quadruple knockout mice leads to severe retinal dysfunction diminished visual motion

In childhood, retinoblastoma (RB) is the most common primary tumor in eye. Long term therapeutic management with etoposide of this life-threatening condition may have diminishing effectiveness since RB cells can develop cytostatic resistance to drug. To determine whether changes receptor-mediated control Ca2+ signaling are associated development, fluorescence calcium imaging, semi-quantitative RT-qPCR analyses, and trypan blue dye exclusion staining patterns compared WERI-ETOR...

There are indications that pharmacological doses of ascorbate (Asc) used as an adjuvant improve the chemotherapeutic management cancer. This favorable outcome stems from its cytotoxic effects due to prooxidative mechanisms. Since regulation intracellular Ca2+ levels contributes maintenance cell viability, we hypothesized one Asc includes disrupting homeostasis. Accordingly, determined if induced influx through activation pertussis sensitive Gi/o-coupled GPCR which in turn activated transient...