A5067046258- Oral microbiology and periodontitis research
- Dental Health and Care Utilization
- Gut microbiota and health
- Dental Research and COVID-19
- Dental Erosion and Treatment
- Probiotics and Fermented Foods
- Dental materials and restorations
- Dental Anxiety and Anesthesia Techniques
- Optical Imaging and Spectroscopy Techniques
- Anaerobic Digestion and Biogas Production
- Genomics and Phylogenetic Studies
- Oral and gingival health research
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Identification and Susceptibility Testing
- Salivary Gland Disorders and Functions
- Virology and Viral Diseases
- Microbial Metabolism and Applications
- Digestive system and related health
- Pediatric health and respiratory diseases
- Streptococcal Infections and Treatments
- Endodontics and Root Canal Treatments
- Medical and Biological Sciences
- Legionella and Acanthamoeba research
- Laser Applications in Dentistry and Medicine
- Infections and bacterial resistance
Méditerranée Infection Foundation
2018-2025
Institut de Recherche pour le Développement
2019-2025
Aix-Marseille Université
2010-2025
Assistance Publique Hôpitaux de Marseille
2019-2023
Hôpital Nord
2023
Université de Montpellier
2019
Laboratoire Bioingénierie et Nanosciences
2018-2019
Hôpital de la Timone
2012
Architecture et Fonction des Macromolécules Biologiques
2010
Clostridium tetani (C. tetani) bacteraemia is a rare situation, with only four case reports in the literature. Fourteen teeth from 1590 plague site Fédons, France, were surface decontaminated before pulp was cultured under strict anaerobiosis negative controls. Colonies identified by mass spectrometry and whole genome sequencing, C. tetani-specific PCR performed using DNA extracted dental pulps, calculus sediments. two specimens individuals firmly MALDI-TOF spectrometry, sequencing confirmed...
The oral fluid microbiome comprises an important bacterial diversity, yet the presence of archaea has not been reported so far. In order to quest for methanogenic (methanogens) in fluid, we used a polyphasic approach including PCR-sequencing detection, microscopic observation by fluorescence in-situ hybridization, isolation and culture, molecular identification genotyping methanogens 200 specimens. negative controls, 64/200 (32%) prospectively analysed specimens were PCR-positive...
Trichomonas tenax, an anaerobic protist difficult to cultivate with unreliable molecular identification, has been suspected of involvement in periodontitis, a multifactorial inflammatory dental disease affecting the soft tissue and bone periodontium. A cohort 106 periodontitis patients classified by stages severity 85 healthy adult control was constituted. An efficient culture protocol, new identification tool real-time qPCR T. tenax Multi-Locus Sequence Typing system (MLST) based on NIH4...
Methanogens have already been described in periodontitis but not peri-implantitis. Thirty peri-implantitis samples and 28 control were collected consenting patients. PCR-sequencing of the 16S rRNA gene was used as a broad-spectrum screening method results further confirmed by real-time quantitative PCR targeting mcrA genes. Results showed methanogen community dominated Methanobrevibacter oralis 31/58 (51%) including 16/28 (57%) 15/30 (50%) samples. massiliense detected 5/58 (8.6%) 3/28 (1%)...
The microbial communities of the oral fluid are in direct contact with tobacco smoke, which may thus affect these communities. Few culture-based studies have analyzed effects smoking on microbiota. Using bacterial culture we investigated whether altered diversity fluid, focusing aerobic and facultative anaerobic Gram-positive bacteria otherwise comprising major pathogens. Among 90 specimens collected 19 tobacco-smokers 71 controls, did not significantly differ age sex. However, was lower...
Among oral microbiota methanogens,
A new and innovative therapeutic concept using a light-induced fluorescence evaluator for diagnosis treatment (LIFEDT) of dental caries based on the imaging autofluorescence tissues is proposed. The aims this series in vivo experiments are to compare analyze brightness variations sound dentin active arrested carious illuminated with an intraoral LED camera determine if device could be helpful daily practice discriminate between dentin.A that emits visible blue light was used study illuminate...
White lesions represent an early phase of caries formation. 20 human sound premolars were subjected to pH cycling procedure induce subsurface (SLs) in vitro. In addition, 2 teeth with naturally developed white spot (WSLs) used as references. All specimens characterized by confocal Raman microscopy being for the first time examining & and providing a high resolution chemical morphological map based on phosphate peak intensity alterations at 960 cm
Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane is, therefore, of paramount importance for equilibrium microbiota. The isolation by culture M. has routinely relied upon hydrogen‑carbon dioxide-enriched, oxygen-deprived atmospheres. In this study, we developed a medium referred to as "GG", which allowed growth in an atmosphere, with no supply either or carbon dioxide, making it easier detect clinical microbiology laboratories.
Nanoarchaeota, obligate symbiont of some environmental archaea with reduced genomes, have been described in marine thermal vent environments, yet never detected hosts, including humans.Here, using laboratory tools geared towards the detection nanoarchaea PCR-sequencing, WGS, microscopy and culture.We discovered a novel nanoarchaea, Nanopusillus massiliensis, dental plate samples by specific PCR-based assays. Combining fluorescent situ hybridization (FISH) scanning electron disclosed close...
In clinical situations carious dentine tissues can be discriminated by most caries fluorescence detection tools, including a new intra‐oral camera. The objectives of this study were: (i) to analyze the R aman spectra sound, carious, and demineralized dentine, (ii) compare spectral analysis with variation observed when using camera, (iii) evaluate involvement M aillard reaction in variations. first positive hypothesis tested was that obtained camera were closely related. second could linked...
ABSTRACT Methanosphaera stadtmanae was the sole representative to be cultured and detected by molecular methods in human gut microbiota, further associated with digestive respiratory diseases, leaving unknown actual diversity of human-associated species. Here, a novel species, Candidatus massiliense ( Ca . M. massiliense) sp. nov. isolated culture using hydrogen- carbon dioxide-free medium from one feces sample. is non-motile, 850 nm Gram-positive coccus autofluorescent at 420 nm....
Aim: Modern clinical caries management involves early stage diagnosis and should fit with dental health policy.The objective of this study was to achieve detection in enamel dentine a laser-based system (DIAGNOdent™ pen) first secondary new fluorescence intra-oral camera (Soprolife®).A visual inspection loupe used as control.Materials methods: Following the consolidated standards reporting trials recommendations, 628 occlusal fissures were included for analysis. Results:The sensitivity...
A questionnaire survey was recently undertaken among French dental students (FDSs) to investigate their practices, knowledge and opinions in various domains of minimal intervention (MI) cariology. The present work focuses on management deep carious lesions (DCLs).