A5045295359- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Parathyroid Disorders and Treatments
- Bone health and treatments
- Bone and Dental Protein Studies
- Orthopaedic implants and arthroplasty
- Periodontal Regeneration and Treatments
- Alkaline Phosphatase Research Studies
- Bone health and osteoporosis research
- Proteoglycans and glycosaminoglycans research
- Bone Metabolism and Diseases
- dental development and anomalies
- Oral microbiology and periodontitis research
- Mesenchymal stem cell research
- Cancer, Hypoxia, and Metabolism
- Salivary Gland Disorders and Functions
- 3D Printing in Biomedical Research
- Sarcoma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Cancer Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Biomedical Research and Pathophysiology
- Orthodontics and Dentofacial Orthopedics
Tel Aviv University
2024-2025
Rambam Health Care Campus
2022-2025
Stanford University
2019-2022
Université Paris Cité
2013-2018
Assistance Publique – Hôpitaux de Paris
2013-2018
Sorbonne Paris Cité
2013-2018
Sorbonne Université
2014-2018
Hôpital Rothschild
2014-2018
Laboratory Orofacial Pathologies, Imaging and Biotherapies
2013-2018
McGill University
2013-2017
Abstract Therapies using mesenchymal stem cell (MSC) seeded scaffolds may be applicable to various fields of regenerative medicine, including craniomaxillofacial surgery. Plastic compression collagen with MSC has been shown enhance the osteogenic differentiation as it increases fibrillary density. The aim present study was evaluate effects dense gel dental pulp cells (DPSC) on bone regeneration in a rat critical-size calvarial defect model. Two symmetrical full-thickness defects were created...
X-linked hypophosphatemia (XLH) is a rare genetic skeletal disease where increased phosphate wasting in the kidney leads to and prevents normal mineralization of bone dentin. Here, we examined periodontal status 34 adults with XLH separated them according treatment they received for hypophosphatemia. We observed that periodontitis frequency severity were varied treatment. Patients who benefited from an early continuous vitamin D supplementation during their childhood presented less...
While advances in biomineralization have been made recent years, unanswered questions persist on bone- and tooth-cell differentiation, outside-in signaling from the extracellular matrix, link between protein expression mineral deposition. In present study, we validate use of a bioengineered three-dimensional (3D) dense collagen hydrogel scaffold as cell-culture model to explore these questions. Dental pulp progenitor/stem cells human exfoliated deciduous teeth (SHEDs) were seeded into an...
Mutations in PHEX (phosphate-regulating gene with homologies to endopeptidases on the X-chromosome) cause X-linked familial hypophosphatemic rickets (XLH), a disorder having severe bone and tooth dentin mineralization defects. The absence of functional leads abnormal accumulation ASARM (acidic serine- aspartate-rich motif) peptide - substrate for strong inhibitor derived from MEPE (matrix extracellular phosphoglycoprotein) other matrix proteins. MEPE-derived accumulates XLH patients where it...
ABSTRACT Aim To evaluate the impact of macrophage depletion on bone loss and inflammatory responses in a mouse model peri‐implantitis, assessing potential as therapeutic strategy. Materials Methods Using 6‐week‐old male C57BL/6 mice, peri‐implantitis was induced by placing silk ligature around osteointegrated dental implants. Mice were divided into three groups: Healthy control (Healthy group); with liposomal phosphate‐buffered saline (PBS clodronate for (CLOD group). Two weeks after...
Mutations in phosphate-regulating gene (PHEX) lead to X-linked hypophosphatemic rickets (XLH), a genetic disease characterized by impaired mineralization bones and teeth. In human XLH tooth dentin, calcospherites that would normally merge as part of the process are separated unmineralized interglobular spaces where fragments matrix proteins accumulate. Here, we immunolocalized osteopontin (OPN) teeth, three-dimensional dental pulp stem cell-collagen scaffold culture model rat injury repair...
X-linked hypophosphatemia (XLH) is a skeletal disorder arising from mutations in the PHEX gene, transmitted most cases as an dominant trait. deficiency leads to renal phosphate wasting and hypophosphatemia, well impaired mineralization of bone dentin, resulting severe dental complications. Dentin defects appear characteristic, large interglobular spaces lack fusion calculospherites circumpulpal region during process. Here, we examined changes composition structure dentin using Raman...
The preservation of bone viability at an osteotomy site is a critical variable for subsequent implant osseointegration. Recent biomechanical studies evaluating the consequences preparation led us to rethink design bone-cutting drills, especially those intended preparation. We present here novel drill that designed efficiently cut very low rotational velocity, obviating need irrigation as coolant. low-speed cutting produces little heat and, consequently, osteocyte maintained. lack irrigation,...
X-linked hypophosphatemia (XLH) is a skeletal disease caused by inactivating mutations in the PHEX gene. Mutated or absent protein/enzyme leads to decreased serum phosphate level, which cause mineralization defects skeleton and teeth (osteomalacia/odontomalacia). It not yet altogether clear whether these manifestations are solely insufficient circulating availability for also direct, local intrinsic effect impaired activity. Here, we evaluated role of 3-dimensional model extracellular matrix...
Introduction: Few interspecies comparisons of alveolar bone have been documented, and this knowledge gap raises questions about which animal models most accurately represent human dental conditions or responses to surgical interventions. Objectives: The objective study was employ state-of-the-art quantitative metrics directly assess compare the structural functional characteristics among humans, mini pigs, rats, mice. Methods: same anatomic location (i.e., posterior maxillae) analyzed in all...
Abstract Background Bone debris generated during site preparation is generally evacuated with irrigation; here, we evaluated whether retention of this autologous material improved the rate peri‐implant bone formation. Methods In 25 rats, a miniature implant system composed an osseo‐shaping tool and tri‐oval‐shaped was compared against conventional drill round system. A split‐mouth design used, fresh extraction sockets served as sites. Histology/histomorphometry, immunohistochemistry,...
Autologous bone can be harvested from the flutes of a conventional drill or scraper; here we compared whether autologous chips generated by new slow-speed instrument were more osteogenic than drills scrapers. Additionally, tested potential could further improved exposure to Wnt signaling (WNT) therapeutic.Osteotomies prepared in fresh rat maxillary first molar extraction sockets using osseo-shaping instrument; titanium alloy implants placed immediately thereafter. Using molecular/cellular...
Osteosarcoma (OS) mortality is attributed to lung metastases. Endothelial progenitor cells (EPCs) mediate the angiogenic switch in several cancers. The spatial proximity between EPCs and OS bone led hypothesis that EPCs-osteosarcoma interactions may possibly promote progression aggressiveness.A PI3K inhibitor, Bevacizumab (an anti-VEGF-A antibody), an anti-FGF2 antibody were added EPCs' conditioned medium (EPC-CM), their impacts on cell (U2-OS 143B) proliferation, migration, invasion, MMP9...
Primary stability is a prerequisite for implant osseointegration. Some degree of misfit between an and its osteotomy required to ensure primary stability, this typically achieved by undersizing osteotomy. In preclinical study, we aimed at understanding the relationship misfit, insertion torque, their cumulative short- longer-term effects on peri-implant bone.We placed implants in maxillary extraction sites rat; control group, these had minimal while those test group high therefore...
Tooth extraction triggers alveolar ridge resorption, and when this resorption is extensive, it can complicate subsequent reconstructive procedures that use dental implants. Clinical data demonstrate the most significant dimensional changes in occur soon after tooth extraction. Here, we sought to understand whether a correlation existed between rate at which an socket heals extent of resorption. Maxillary molars were extracted from young osteoporotic rodents, quantitative micro-computed...
Abstract Objectives Compared to autografts, bone graft substitutes are slower consolidate. If we understood why, this might open strategies accelerate new formation and thus shorten the time implant placement. In study, aimed at comparing autologous with a bovine substitute in preclinical sinus lift model. Materials Methods The mouse posterior paranasal served as recipient site for grafting. Autograft from oral cavity was compared against using molecular, cellular, histological analyses...
Abstract Objectives Oral implants transmit biting forces to peri‐implant bone. In turn, those subject bone mechanical stresses and strains. Here, our objective was understand how responded conditions of normal versus hyper‐loading in a mouse model. Material Methods Sixty‐six mice were randomly assigned 2 groups; both groups underwent bilateral maxillary first molar extraction followed by complete healing. Titanium alloy placed healed sites positioned below the occlusal plane. After...
Background: Medication-related osteonecrosis of the jaw is a serious complication that develops in oncologic patients treated with Zoledronic acid. Although used for over 30 years, influence acid on bone has been thoroughly investigated, mainly osteoclasts. While decreasing osteoclast differentiation and function, many years it was thought increased osteoblast differentiation, thus increasing volume. Moreover, despite soft tissue healing process, impact zoledronic interaction between not...
BackgroundPeriodontitis is a set of chronic inflammatory diseases affecting the supporting structures teeth, during which persistent release lytic enzymes and mediators causes self-perpetuating vicious cycle tissue destruction repair. A matrix-based therapy using heparan sulfate (HS) analogue called ReGeneraTing Agent (RGTA) replaces destroyed HS by binding to available heparin-binding sites structural molecules, leading restoration homeostasis in several injuries, including hamster...
Our objective was to test the hypothesis that local delivery of a WNT protein therapeutic would support osseointegration an unstable implant placed into oversized osteotomy and subjected functional loading.Using split-mouth design in ovariectomized (OVX) rat model, 50 titanium implants were osteotomies. Implants loading. One-half treated with liposomal formulation WNT3A (L-WNT3A); other half received identical containing phosphate-buffered saline (PBS). Finite element modeling estimated...
Abstract The disconnect between preclinical and clinical results underscores the imperative for establishing good animal models, then gleaning all available data on efficacy, safety, potential toxicities associated with a device or drug. Mini pigs are commonly used model testing orthopedic dental devices because their skeletons large enough to accommodate human-sized implants. challenge comes analyses of hard tissues: current methods time-consuming, destructive, largely limited histological...