A5033346630- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Diffusion and Search Dynamics
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Advanced Fluorescence Microscopy Techniques
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Bone Tissue Engineering Materials
- Nanoparticle-Based Drug Delivery
- Near-Field Optical Microscopy
- Vibrio bacteria research studies
- Molecular Junctions and Nanostructures
- Photoreceptor and optogenetics research
- Cancer-related gene regulation
- 3D Printing in Biomedical Research
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
Oxford Nanoimaging (United Kingdom)
2019-2023
Delft University of Technology
2014-2019
Max Planck Institute of Molecular Cell Biology and Genetics
2012
International Institute of Molecular and Cell Biology
2012
Protrusion formation is an essential step during cell migration. Cells migrating in three-dimensional environments and vivo can form a wide variety of protrusion types, including actin polymerization-driven lamellipodia, contractility-driven blebs. The ability to switch between different protrusions has been proposed facilitate motility complex promote cancer dissemination. However, plasticity so far mostly investigated the context transitions amoeboid mesenchymal migration modes, which...
Single-molecule fluorescence spectroscopy has proven to be instrumental in understanding a wide range of biological phenomena at the nanoscale. Important examples what this technique can yield sciences are mechanistic insights on protein-protein and protein-nucleic acid interactions. When interactions proteins probed single-molecule level, or their substrates often immobilized glass surface, which allows for long-term observation. This immobilization scheme may introduce unwanted surface...
Prokaryotic Argonaute proteins (pAgos) constitute a diverse group of endonucleases which some mediate host defense by utilizing small interfering DNA guides (siDNA) to cleave complementary invading DNA. This activity can be repurposed for programmable cleavage. However, currently characterized DNA-cleaving pAgos require elevated temperatures (≥65°C) their activity, making them less suitable applications that moderate temperatures, such as genome editing. Here, we report the functional and...
MicroRNAs (miRNAs) direct post-transcriptional regulation of human genes by guiding Argonaute proteins to complementary sites in messenger RNAs (mRNAs) targeted for repression. An enigmatic feature many conserved mammalian miRNA target is that an adenosine (A) nucleotide opposite nucleotide-1 confers enhanced repression independently base pairing potential the miRNA. In this study, we show Argonaute2 (Ago2) possesses a solvated surface pocket specifically binds adenine nucleobases 1 position...
Single-molecule fluorescence spectroscopy has proven to be instrumental in understanding a wide range of biological phenomena at the nanoscale. Important examples what this technique can yield sciences are mechanistic insights on protein-protein and protein-nucleic acid interactions. When interactions proteins probed single-molecule level, or their substrates often immobilized glass surface, which allows for long-term observation. This immobilization scheme may introduce unwanted surface...
Argonaute (Ago) proteins are key players in both gene regulation (eukaryotes) and host defense (prokaryotes). Acting on single-stranded nucleic-acid substrates, Ago relies base pairing between a small guide its complementary target sequences for specificity. To efficiently scan chains targets, diffuses laterally along the substrate must bypass secondary structures as well protein barriers. Using single-molecule FRET conjunction with kinetic modelling, we reveal that scanning is mediated...
Nanoscale liposomes have been extensively researched and employed clinically for the delivery of biologically active compounds, including chemotherapy drugs vaccines, offering improved pharmacokinetic behaviour therapeutic outcomes. Traditional laboratory-scale production methods often suffer from limited control over liposome properties (e.g., size lamellarity) rely on laborious multistep procedures, which may limit pre-clinical research developments innovation in this area. The widespread...
Abstract Prokaryotic Argonaute proteins (pAgos) constitute a diverse group of endonucleases which some mediate host defense by utilizing small interfering DNA guides (siDNA) to cleave complementary invading DNA. This activity can be repurposed for programmable cleavage. However, currently characterized DNA-cleaving pAgos require elevated temperatures (≥65°C) their activity, making them less suitable applications that moderate temperatures, such as genome editing. Here we report the...
Argonaute (Ago) proteins are key players in gene regulation eukaryotes and host defense prokaryotes. For specific interference, Ago relies on base pairing between small nucleic acid guides complementary target sequences. To efficiently scan chains for potential targets, must bypass both secondary structures mRNA single stranded DNA as well protein barriers. Through single-molecule FRET, we reveal that lateral diffusion is mediated mainly through protein-nucleic interactions, rather than...