A5055513936- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Cellular transport and secretion
- Barrier Structure and Function Studies
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Cell Adhesion Molecules Research
- Microfluidic and Bio-sensing Technologies
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Caveolin-1 and cellular processes
- Nuclear Structure and Function
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Evolution and Genetic Dynamics
- Immune cells in cancer
- Ion Transport and Channel Regulation
- Cancer Research and Treatment
- Protist diversity and phylogeny
- Telomeres, Telomerase, and Senescence
- Axon Guidance and Neuronal Signaling
- Dental Radiography and Imaging
IFOM
2016-2025
Italian Association for Cancer Research
2021-2025
University of Milan
2018-2024
Azienda Sanitaria Unità Locale di Reggio Emilia
2022
ORCID
2021
ER-PM contacts in nonclathrin endocytosis The epidermal growth factor receptor (EGFR) is internalized through both clathrin-mediated and (NCE). two pathways act concert to sustain EGFR signaling or its long-term attenuation. mechanistic underpinnings of EGFR-NCE are unclear. Caldieri et al. used a variety cell molecular biology approaches identify nine regulators (see the Perspective by Tan Anderson). They also identified an additional cargo pathway (CD147). One was endoplasmic reticulum...
Intercellular tight junctions are crucial for correct regulation of the endothelial barrier. Their composition and integrity affected in pathological contexts, such as inflammation tumor growth. JAM-A (junctional adhesion molecule A) is a transmembrane component with role maintenance barrier function, although how this accomplished remains elusive.We aimed to understand molecular mechanisms through which expression regulates junction organization control permeability, potential implications...
Abstract The process in which locally confined epithelial malignancies progressively evolve into invasive cancers is often promoted by unjamming, a phase transition from solid-like to liquid-like state, occurs various tissues. Whether this tissue-level mechanical impacts phenotypes during carcinoma progression remains unclear. Here we report that the large fluctuations cell density accompany unjamming result repeated deformations of cells and nuclei. This triggers cellular mechano-protective...
Abstract Circulating tumor cell (CTC) clusters are associated with increased metastatic potential and worse patient prognosis, but rare, difficult to count, poorly characterized biophysically. The PillarX device described here is a bimodular microfluidic (Pillar‐device an X‐magnetic device) profile single CTCs from whole blood based on their size, deformability, epithelial marker expression. Larger, less deformable large cells captured in the Pillar‐device sorted according pillar gap sizes....
Therapeutic options for patients with advanced-stage hepatocellular carcinoma (HCC) are very limited. The only approved first-line treatment is the multi-tyrosine kinase inhibitor sorafenib, which shows low response rates and severe side effects. In particular, compensatory activation of growth factor receptors leads to chemoresistance limits clinical impact sorafenib. However, combination approaches improve sorafenib have failed. Here we investigate inhibition cyclin-dependent 5 (Cdk5) as a...
Abstract During tumorigenesis, the extracellular matrix (ECM), which constitutes structural scaffold of tissues, is profoundly remodeled. While impact such remodeling on tumor growth and invasion has been extensively investigated, much less known consequences ECM infiltration by immune cells. By combining tissue imaging machine-learning, we here show that localization T lymphocytes neutrophils, orchestrate antitumor responses, can be predicted defined topographical features fibrillar...
<title>Abstract</title> Tissue-level phase transitions are emerging as a crucial mechanism in tumour development and metastasis. This study aims to identify molecular determinants physical conditions that control active wetting solid-to-fluid transition of epithelial tissues. We focused on IRSp53, protein linking plasma membranes the cytoskeleton. Depleting MCF10 DCIS.com cells, disrupts coordinated collective movement by promoting local fluctuations cell velocity resulting increased tissue...
Abstract Mitochondria change distribution across cells following a variety of pathophysiological stimuli. The mechanisms presiding over this redistribution are yet undefined. In murine model overexpressing Drp1 specifically in skeletal muscle, we find marked mitochondria repositioning muscle fibres and demonstrate that is involved process. binds KLC1 enhances microtubule-dependent transport mitochondria. Drp1-KLC1 coupling triggers the displacement KIF5B from kinesin-1 complex increasing its...
Although human nucleoporin Tpr is frequently deregulated in cancer, its roles are poorly understood. Here we show that depletion generates transcription-dependent replication stress, DNA breaks, and genomic instability. fiber assays electron microscopy visualization of intermediates deficient cells exhibit slow asymmetric forks under stress. deficiency evokes enhanced levels DNA-RNA hybrids. Additionally, complementary proteomic strategies identify a network Tpr-interacting proteins...
Abstract Alternative lengthening of telomeres (ALT) is a telomere maintenance mechanism activated in ~10–15% cancers, characterized by telomeric damage. Telomeric damage-induced long non-coding RNAs (dilncRNAs) are transcribed at dysfunctional and contribute to DNA damage response (DDR) activation repair. Here we observed that dilncRNAs preferentially elevated ALT cells. Inhibition C-rich (teloC) with antisense oligonucleotides leads replication stress responses, increased genomic...
Several Alveolar Ridge Preservation (ARP) procedures have been proposed over the years. The purpose of this study was to describe new Modified Periosteal Inhibition (MPI) technique for ARP. Seven patients were enrolled (age range: 28–72 years old; 5 males, 2 females). In total, nine hopeless teeth treated. Following elevation a full-thickness flap, atraumatic tooth extraction conducted, preserving buccal bone alveolar socket. OsteoBiol® Lamina Soft (Tecnoss®, Giaveno, Italy), 0.5 mm thick,...
Cancers feature substantial intratumoral heterogeneity of genetic and phenotypically distinct lineages. Although interactions between coexisting lineages are emerging as a potential contributor to tumor evolution, the extent nature these remain largely unknown. We postulated that tumors develop ecological sustain diversity facilitate metastasis. Using combination fluorescent barcoding, mathematical modeling, metabolic analysis, in vivo models, we show Allee effect, i.e., growth dependency on...
Abstract Specialised ribonucleoprotein (RNP) granules are a hallmark of polarized cells, like neurons and germ cells. Among their main functions is the spatial temporal modulation activity specific mRNA transcripts that allow specification primary embryonic axes. While RNPs composition role well established, regulation poorly defined. Here, we demonstrate Hecw, newly identified Drosophila ubiquitin ligase, key modulator in oogenesis neurons. Hecw depletion leads to formation enlarged...
The use of electromagnetic (EM) technologies for military applications is gaining increasing interest to satisfy different operational needs, such as improving battlefield communications or jamming counterpart's signals. This achieved by the high-power EM waves in several frequency bands (e.g., HF, VHF, and UHF). When considering vehicles, antennas are present close proximity crew personnel, which thus potentially exposed high fields.A typical exposure scenario was reproduced numerically...
Abstract During wound repair, branching morphogenesis and carcinoma dissemination, cellular rearrangements are fostered by a solid-to-liquid transition known as unjamming. The biomolecular machinery behind unjamming, its physiological clinical relevance remain, however, mystery. Here, we combine biophysical biochemical analysis to study unjamming in variety of epithelial 2D 3D collectives: monolayers, differentiated normal mammary cysts, spheroid models breast ductal situ (DCIS), ex vivo...
Tumor-to-stroma ratio (TSR) is a prognostic factor that expresses the relative amounts of tumor and intratumoral stroma. In this study, its clinical molecular relevance was evaluated in prostate cancer (PCa). The feasibility automated quantification tested digital scans tissue microarrays containing 128 primary tumors from 72 PCa patients stained immunohistochemically for epithelial cell adhesion molecule (EpCAM), followed by validation cohort 310 209 patients. order to investigate gene...
The blood-brain barrier (BBB) acquires unique properties to regulate neuronal function during development. formation of the BBB, which occurs in tandem with angiogenesis, is directed by Wnt/β-catenin signaling pathway. Yet exact molecular interplay remains elusive. Our study reveals G protein–coupled receptor GPR126 as a critical target canonical Wnt signaling, essential for development BBB's distinctive vascular characteristics and its functional integrity. Endothelial cell–specific...
Specialised ribonucleoprotein (RNP) granules are a hallmark of germ cells. Among their main function is the spatial and temporal modulation activity specific mRNA transcripts that allow specification primary embryonic axes. While RNPs composition role well established, regulation poorly defined. Here, we demonstrate Hecw, newly identified Drosophila ubiquitin ligase, key modulator in oogenesis. Loss Hecw results formation enlarged transition from liquid to gel-like state. At molecular level,...