A5011342277- Sphingolipid Metabolism and Signaling
- Autophagy in Disease and Therapy
- Lipid Membrane Structure and Behavior
- Nanoplatforms for cancer theranostics
- Muscle Physiology and Disorders
- Photochromic and Fluorescence Chemistry
- Immune cells in cancer
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Calcium signaling and nucleotide metabolism
- Nitric Oxide and Endothelin Effects
- Neuroinflammation and Neurodegeneration Mechanisms
- Protist diversity and phylogeny
- Extracellular vesicles in disease
- Adenosine and Purinergic Signaling
- Cellular transport and secretion
- Retinal Diseases and Treatments
- Advanced Glycation End Products research
- Cannabis and Cannabinoid Research
- Lipid metabolism and biosynthesis
- Immune Response and Inflammation
- Receptor Mechanisms and Signaling
- Phagocytosis and Immune Regulation
University of Milan
2016-2025
Luigi Sacco Hospital
2009-2023
ASST Fatebenefratelli Sacco
2022
National Research Council
2013-2015
IRCCS Eugenio Medea
2005-2013
San Raffaele University of Rome
2005-2007
Academia Nacional de Medicina
2006
University of Calabria
2003-2005
University of Florence
2005
Vita-Salute San Raffaele University
2002-2004
The endocrine system participates in regulating macrophage maturation, although little is known about the modulating role of thyroid hormones. In vitro results demonstrate a negative one such hormone, triiodothyronine (T3), triggering differentiation bone marrow–derived monocytes into unpolarized macrophages. T3-induced macrophages displayed classically activated (M1) signature. A M1-priming effect was also observed on polarized because T3 reverses alternatively (M2) activation, whereas it...
Tumor microenvironment is fundamental for cancer progression and chemoresistance. Among stromal cells tumor-associated macrophages (TAMs) represent the largest population of infiltrating inflammatory in malignant tumors, promoting their growth, invasion immune evasion. M2-polarized TAMs are endowed with NO generating enzyme iNOS. has divergent effects on since it can either stimulate tumor growth or promote death depending source it; likewise role iNOS differs cell type. The generated by TAM...
Objective— Tumor necrosis factor α (TNF-α), a key proinflammatory cytokine acting on the endothelium, activates endothelial nitric oxide synthase (eNOS). We have examined signaling pathway leading to this activation and its biological role in which are still unknown. Methods Results— In human cells, we found that eNOS by TNF-α is time dependent requires of Akt, known activator. was preceded sequential neutral-sphingomyelinase-2 (N-SMase2) sphingosine-kinase-1 (SK1) generation...
Duchenne muscular dystrophy is a relatively common disease that affects skeletal muscle, leading to progressive paralysis and death. There currently no resolutive therapy. We have developed treatment in which we combined the effects of nitric oxide with nonsteroidal antiinflammatory activity by using HCT 1026, oxide-releasing derivative flurbiprofen. Here, report results long-term (1-year) oral 1026 two murine models for limb girdle dystrophies (alpha-sarcoglycan-null mdx mice). In both...
Autophagy occurs at a basal level in all eukaryotic cells and may support cell survival or activate death pathways. Due to its pathophysiologic significance, the autophagic machinery is promising target for development of multiple approaches anti-neoplastic agents. We have recently described cytotoxic pro-apoptotic mechanisms, targeting tumour suppressor p53, climacostol, natural product ciliated protozoan Climacostomum virens. report here on how climacostol regulates autophagy involvement...
Nitric oxide (NO), generated in skeletal muscle mostly by the neuronal NO synthases (nNOSμ), has profound effects on both mitochondrial bioenergetics and development function. The importance of for repair emerges from observation that nNOS signalling is defective many genetically diverse diseases which dysregulated. How NO/nNOSμ mitochondria impact function, however, not been investigated yet.In this study we have examined relationship between system, structure/activity...
This paper focuses on recent advancements in the development of 4D printed drug delivery systems (DDSs) for intravesical administration drugs. By coupling effectiveness local treatments with major compliance and long-lasting performance, they would represent a promising innovation current treatment bladder pathologies. Being based shape-memory pharmaceutical-grade polyvinyl alcohol (PVA), these DDSs are manufactured bulky shape, can be programmed to take collapsed one suitable insertion into...
Abstract Depletion of dendritic cells (DCs) via apoptosis contributes to sepsis-induced immune suppression. The mechanisms leading DC during sepsis are not known. In this study we report that immature DCs undergo when treated with high numbers Escherichia coli. This effect was mimicked by concentrations LPS. Apoptosis accompanied generation ceramide through activation acid sphingomyelinase (A-SMase), prevented inhibitors enzyme, and restored exogenous ceramide. Compared DCs, mature expressed...
Activation of endothelial nitric-oxide synthase (eNOS) has been shown to occur through various pathways involving increases in the cytosolic Ca<sup>2+</sup> concentration, activation phosphatidylinositol-3′ kinase/Akt pathway, as well regulation by other kinases and protein-protein interactions. We have recently reported that eNOS, expressed an inducible HeLa Tet-off cell line, is activated tumor necrosis factor-α (TNF-α) a previously undescribed pathway involves lipid messenger ceramide....
Acid sphingomyelinase (A-SMase) is an important enzyme in sphingolipid metabolism and plays key roles apoptosis, immunity, development, cancer. In addition, it mediates cytotoxicity of cisplatin some other chemotherapeutic drugs. The mechanism A-SMase activation still undefined. We now demonstrate that, upon CD95 stimulation, activated through translocation from intracellular compartments to the plasma membrane exocytic pathway requiring t-SNARE protein syntaxin 4. Indeed, down-regulation 4...
The late-infantile-onset forms of neuronal ceroid lipofuscinosis (LINCL) are the most genetically heterogeneous group among autosomal recessive lipofuscinoses (NCLs), with causative mutations found in CLN1, CLN2, CLN5, CLN6, CLN7 (MFSD8), and CLN8 genes. Homozygous associated two distinct phenotypes: progressive epilepsy mental retardation (EPMR), first identified Finland; a variant late-infantile NCL (v-LINCL) described subset Turkish Italian patients. function protein encoded by is...
Signals released by leukocytes contribute to tumor growth and influence the efficacy of antineoplastic treatments. The outcome peritoneal carcinomatosis treatments is unsatisfactory, possibly because chemotherapy activates events that have in long‐term deleterious effects. In this study we offer evidence 5‐fluorouracile (5‐FU), besides provoking apoptosis MC38 colon carcinoma cells, induces a striking attraction both an orthotopic model vivo monocyte‐migration assays vitro . Leukocyte...