A5031903259- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
- Caveolin-1 and cellular processes
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- Lysosomal Storage Disorders Research
- Erythrocyte Function and Pathophysiology
- Biomedical Research and Pathophysiology
- Phagocytosis and Immune Regulation
- Autophagy in Disease and Therapy
- ATP Synthase and ATPases Research
- Ginger and Zingiberaceae research
- Pancreatic function and diabetes
- Nitric Oxide and Endothelin Effects
- Immune cells in cancer
- Adenosine and Purinergic Signaling
- Microtubule and mitosis dynamics
- Lipid metabolism and biosynthesis
- Metabolism, Diabetes, and Cancer
- Cancer, Lipids, and Metabolism
- Cholesterol and Lipid Metabolism
- Pharmacological Effects of Natural Compounds
- COVID-19 and Mental Health
University of Milan
2016-2025
Institute of Biomedical Technologies
2013-2024
Tecnologie Avanzate (Italy)
2012
Virginia Commonwealth University
2004-2007
Microglia are highly plastic immune cells which exist in a continuum of activation states. By shaping the function oligodendrocyte precursor (OPCs), brain differentiate to myelin-forming cells, microglia participate both myelin injury and remyelination during multiple sclerosis. However, mode(s) action supporting or inhibiting repair is still largely unclear. Here, we analysed effects extracellular vesicles (EVs) produced vitro by either pro-inflammatory pro-regenerative on OPCs at...
The mitogenic role of sphingosine-1-phosphate (S1P) and its involvement in basic fibroblast growth factor (bFGF)-induced proliferation were examined primary cultures cerebellar astrocytes. Exposure to bFGF resulted a rapid increase extracellular S1P formation, inducing astrocytes release S1P, but not sphingosine kinase, the milieu. SK inhibitor N,N-dimethylsphingosine inhibited as well bFGF-induced stimulation. application quiescent caused dose-dependent DNA synthesis. This gliotrophic...
In yeast, the long-chain sphingoid base phosphate phosphohydrolase Lcb3p is required for efficient ceramide synthesis from exogenous bases. Similarly, in this study, we found that incorporation of sphingosine into mammalian cells was regulated by homologue Lcb3p, sphingosine-1-phosphate 1 (SPP-1), an endoplasmic reticulum resident protein. Sphingosine endogenous ceramides increased SPP-1 overexpression, whereas recycling C(6)-ceramide not altered. The increase inhibited fumonisin B(1),...
Growing evidence indicates that sphingosine-1-P (S1P) upregulates glutamate secretion in hippocampal neurons. However, the molecular mechanisms through which S1P enhances excitatory activity remain largely undefined. The aim of this study was to identify presynaptic targets action controlling exocytosis. Confocal analysis rat neurons showed applied at nanomolar concentration alters distribution Synapsin I (SynI), a phosphoprotein controls availability synaptic vesicles for induced SynI...
Introduction Microglia and macrophages can influence the evolution of myelin lesions through production extracellular vesicles (EVs). While microglial EVs promote in vitro differentiation oligodendrocyte precursor cells (OPCs), whether derived from aid or limit OPC maturation is unknown. Methods Immunofluorescence analysis for protein MBP was employed to evaluate impact primary rat on cultured differentiation. Raman spectroscopy liquid chromatography-mass spectrometry used define...
Accumulating reports suggest that human glioblastoma contains glioma stem‐like cells (GSCs) which act as key determinants driving tumor growth, angiogenesis, and contributing to therapeutic resistance. The proliferative signals involved in GSC proliferation progression remain unclear. Using lines derived from specimens with different index stemness marker expression, we assessed the hypothesis sphingosine‐1‐phosphate (S1P) affects properties of GSCs. results metabolic studies demonstrated...
Glioblastomas are the most frequent and aggressive intracranial neoplasms in humans, despite advances introduction of alkylating agent temozolomide therapy have improved patient survival, resistance mechanisms limit benefits. Recent studies support that glioblastoma stem-like cells (GSCs), a cell subpopulation within tumour, involved aberrant expansion properties glioblastomas, through still unclear mechanisms. Emerging evidence suggests sphingosine-1-phosphate (S1P) potent onco-promoter...
One main mechanism of insulin resistance (IR), a key feature type 2 diabetes, is the accumulation saturated fatty acids (FAs) in muscles obese patients with diabetes. Understanding that underlies lipid-induced IR an important challenge. Saturated FAs are metabolized into lipid derivatives called ceramides, and their plays central role development muscle IR. Ceramides produced endoplasmic reticulum (ER) transported to Golgi apparatus through transporter CERT, where they converted various...
Lysosomal accumulation of undegraded materials is a common feature lysosomal storage diseases, neurodegenerative disorders, and the aging process. To better understand role in onset cell damage, we used human fibroblasts loaded with sucrose as model accumulation. Sucrose-loaded displayed increased biogenesis followed by arrested proliferation. Notably, found that reduced catabolism autophagy impairment led to an increase sphingolipids (i.e., sphingomyelin, glucosylceramide, ceramide,...
Glioblastoma is a deadly cancer with intrinsic chemoresistance. Understanding this property will aid in therapy. Glucosylceramide synthase (GCS) associated resistance and poor outcome; little known about glioblastomas. In glioblastoma cells, temozolomide paclitaxel induce ceramide increase, which turn promotes cytotoxicity. drug-resistant both drugs are unable to accumulate ceramide, increased expression activity of GCS present, its inhibitors hinder resistance. Resistant cells exhibit...
Autophagic impairment was identified in many lysosomal storage diseases and adult neurodegenerative diseases. It seems that this defect could be directly related to the appearance of a phenotype contribute worsen metabolite accumulation distress. Thus, autophagy is becoming promising target for supportive therapies. Autophagy alterations were recently also Krabbe disease. disease characterized by extensive demyelination dysmyelination it due genetic loss function enzyme galactocerebrosidase...