A5031397349- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Dietary Effects on Health
- Marine Ecology and Invasive Species
- Nutrition, Genetics, and Disease
- Diet and metabolism studies
- DNA Repair Mechanisms
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Genetic factors in colorectal cancer
- Telomeres, Telomerase, and Senescence
- T-cell and B-cell Immunology
- Identification and Quantification in Food
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Cellular Mechanics and Interactions
- Lymphoma Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Glioma Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
European Institute of Oncology
2010-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2024-2025
IFOM
2016-2024
Italian Association for Cancer Research
2024
Ripamonti
2024
Alexander Fleming Biomedical Sciences Research Center
2024
ETH Zurich
2024
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2024
University of Milan
2004-2014
RELX Group (United Kingdom)
2007
DNA double-strand breaks (DSBs) are toxic lesions, which, if not properly repaired, may lead to genomic instability, cell death and senescence. Damage-induced long non-coding RNAs (dilncRNAs) transcribed from broken ends contribute damage response (DDR) signaling. Here we show that dilncRNAs play a role in DSB repair by homologous recombination (HR) contributing the recruitment of HR proteins BRCA1, BRCA2, RAD51, without affecting DNA-end resection. In S/G2-phase cells, pair resected form...
Abstract In tumor-bearing mice, cyclic fasting or fasting-mimicking diets (FMD) enhance the activity of antineoplastic treatments by modulating systemic metabolism and boosting antitumor immunity. Here we conducted a clinical trial to investigate safety biological effects cyclic, five-day FMD in combination with standard therapies. 101 patients, was safe, feasible, resulted consistent decrease blood glucose growth factor concentration, thus recapitulating metabolic changes that mediate...
Abstract Of the many types of DNA damage, double-strand breaks (DSBs) are probably most deleterious. Mounting evidence points to an intricate relationship between DSBs and transcription. A cell system in which impact on transcription can be investigated at precisely mapped genomic is essential study this relationship. Here a human line, we map genome-wide high resolution induced by restriction enzyme, characterize their gene expression four independent approaches monitoring steady-state RNA...
Metastatic tumors remain lethal due to primary/acquired resistance therapy or cancer stem cell (CSC)-mediated repopulation. We show that a fasting-mimicking diet (FMD) activates starvation escape pathways in triple-negative breast (TNBC) cells, which can be identified and targeted by drugs. In CSCs, FMD lowers glucose-dependent protein kinase A signaling stemness markers reduce number increase mouse survival. Accordingly, metastatic TNBC patients with lower glycemia survive longer than those...
Abstract The DNA damage response (DDR) is a set of cellular events that follows the generation damage. Recently, site-specific small non-coding RNAs, also termed RNAs (DDRNAs), have been shown to play role in DDR signalling and repair. Dysfunctional telomeres activate ageing, cancer an increasing number identified pathological conditions. Here we show that, mammals, telomere dysfunction induces transcription telomeric DDRNAs (tDDRNAs) their longer precursors from both strands. activation...
Primary Epstein-Barr virus (EBV)-positive nodal T/NK-cell lymphoma (PTCL-EBV) is a poorly understood disease which shows features resembling extranodal NK/T-cell (ENKTL) and currently not recognized as distinct entity but categorized variant of primary T-cell otherwise specified (PTCL-NOS). Herein, we analyzed copynumber aberrations (n=77) with focus on global measures genomic instability homologous recombination deficiency performed gene expression (n=84) EBV miRNA (n=24) profiling well...
Abstract The process in which locally confined epithelial malignancies progressively evolve into invasive cancers is often promoted by unjamming, a phase transition from solid-like to liquid-like state, occurs various tissues. Whether this tissue-level mechanical impacts phenotypes during carcinoma progression remains unclear. Here we report that the large fluctuations cell density accompany unjamming result repeated deformations of cells and nuclei. This triggers cellular mechano-protective...
Immunotherapy is improving the prognosis and survival of cancer patients, but despite encouraging outcomes in different cancers, majority tumors are resistant to it, immunotherapy combinations often accompanied by severe side effects. Here, we show that a periodic fasting-mimicking diet (FMD) can act on tumor microenvironment increase efficacy (anti-PD-L1 anti-OX40) against poorly immunogenic triple-negative breast (TNBCs) expanding early exhausted effector T cells, switching metabolism from...
Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC to peritoneum. mechanisms underlying interactions between metastatic cells and ECM, however, remain poorly understood, number vitro models available for study peritoneal process is limited. Here, we show that decellularized ECM cavity allows growth organoids obtained PM, favoring development three-dimensional (3D)...
Abstract Synchronous colorectal cancers (syCRCs) are physically separated tumours that develop simultaneously. To understand how the genetic and environmental background influences development of multiple tumours, here we conduct a comparative analysis 20 syCRCs from 10 patients. We show have independent origins, acquire dissimilar somatic alterations, different clone composition. This inter- intratumour heterogeneity must be considered in selection therapy monitoring resistance. SyCRC...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the disease 2019 (COVID-19), known to be more common in elderly, who also show symptoms and are at higher risk of hospitalization death. Here, we that expression angiotensin converting enzyme (ACE2), SARS-CoV-2 cell receptor, increases during aging mouse human lungs. ACE2 upon telomere shortening or dysfunction both cultured mammalian cells vivo mice. This increase is controlled transcriptional level, Ace2 promoter...
Abstract Phosphatidylinositol-5-phosphate (PtdIns5P)−4-kinases (PIP4Ks) are stress-regulated phosphoinositide kinases able to phosphorylate PtdIns5P PtdIns(4,5)P2. In cancer patients their expression is typically associated with bad prognosis. Among the three PIP4K isoforms expressed in mammalian cells, PIP4K2B one more prominent nuclear localisation. Here, we unveil role of as a mechanoresponsive enzyme. protein level strongly decreases cells growing on soft substrates. Its direct silencing...
Ascidians are a fascinating group of filter-feeding marine chordates characterized by rapid evolution both sequences and structure their nuclear mitochondrial genomes. Moreover, they include several model organisms used to investigate complex biological processes in chordates. To study the evolutionary dynamics ascidians at short phylogenetic distances, we sequenced 13 new mitogenomes analyzed them, together with 15 other available mitogenomes, using novel approach involving detailed...
We applied digital spatial profiling for 87 immune and stromal genes to lymph node germinal center (GC) dark- light-zone (DZ/LZ) regions of interest obtain a differential signature these two distinct microenvironments. The spatially resolved 53-genes signature, comprising key the DZ mutational machinery LZ mesenchymal milieu, was transcriptomes 543 GC-related diffuse large B cell lymphomas double-hit (DH) lymphomas. According DZ/LZ were sub-classified into clusters. subgroups differed in...