A5037446721- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Tendon Structure and Treatment
- Neuroscience and Neural Engineering
- Axon Guidance and Neuronal Signaling
- Near-Field Optical Microscopy
- Intraperitoneal and Appendiceal Malignancies
- Erythrocyte Function and Pathophysiology
- Mechanical and Optical Resonators
- Microtubule and mitosis dynamics
- Metal and Thin Film Mechanics
- Lipid Membrane Structure and Behavior
- Microfluidic and Bio-sensing Technologies
- Pancreatic and Hepatic Oncology Research
- Caveolin-1 and cellular processes
- Heat shock proteins research
- Adhesion, Friction, and Surface Interactions
- Hernia repair and management
- Laser-Ablation Synthesis of Nanoparticles
- Polymer Surface Interaction Studies
- Analytical Chemistry and Sensors
- Trauma, Hemostasis, Coagulopathy, Resuscitation
University of Milan
2018-2023
Mylan (South Africa)
2021
Mylan (Switzerland)
2020
Interface (United States)
2019
Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC to peritoneum. mechanisms underlying interactions between metastatic cells and ECM, however, remain poorly understood, number vitro models available for study peritoneal process is limited. Here, we show that decellularized ECM cavity allows growth organoids obtained PM, favoring development three-dimensional (3D)...
Cell stiffening induced by anticancer microtubule targeting drugs (MTDs) such as vinflunine (VFL), colchicine (COL), and docetaxel (DTX) can be driven crosstalk between actin filaments microtubules.
Biosensors are aimed at detecting tiny physical and chemical stimuli in biological systems. Physical forces ubiquitous, being implied all cellular processes, including cell adhesion, migration, differentiation. Given the strong interplay between cells their microenvironment, extracellular matrix (ECM) structural mechanical properties of ECM play an important role transmission external to single within tissue. Vice versa, themselves also use self-generated probe biophysical ECM. mechanics...
Abstract Tissue mechanics determines tissue homeostasis, disease development and progression. Bladder strongly relies on its mechanical properties to perform physiological function, but these are poorly unveiled under normal pathological conditions. Here we characterize the fingerprints at micro-scale level of three layers which compose healthy bladder wall, identify modifications associated with onset progression conditions (i.e., actinic cystitis cancer). We use two indentation-based...
Abstract Atomic force microscopy (AFM) is a powerful tool to investigate interaction forces at the micro and nanoscale. Cantilever stiffness, dimensions geometry of tip can be chosen according requirements specific application, in terms spatial resolution sensitivity. Colloidal probes (CPs), obtained by attaching spherical particle tipless (TL) cantilever, offer several advantages for accurate measurements: tunable well‐characterisable radius; higher averaging capabilities (at expense...
Abstract The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many details mechanotransductive signal integration remain elusive due to complexity involved (extra)cellular structures, such as glycocalyx. We used nano-bio-interfaces reproducing complex nanotopographical features extracellular matrix analyse glycocalyx impact on PC12 cell mechanosensing at nanoscale ( e.g. , by force spectroscopy with functionalised probes). Our data...
Abstract Peritoneal metastases (PM) are common routes of dissemination for colorectal cancer (CRC) and remain a lethal disease with poor prognosis. The properties the extracellular matrix (ECM) important in development; studying their changes is crucial to understand CRC-PM development. We studied elastic ECMs derived from human samples normal neoplastic PM by atomic force microscopy (AFM); results were correlated patient clinical data expression ECM components related metastatic spread....
The study shows, by exploiting a novel adhesion force spectroscopy approach, that microenvironmental nanotopography impacts strongly on integrin-mediated cellular mechanosensing, influencing site loading dynamics.
Artificially grown neuronal cultures of brain cells have been used for decades in the attempt to reproduce and study vitro complexity circuits. It soon became evident that this alone was insufficient, because random architecture these artificial networks. Important groundwork therefore resulted development methods confine adhesion at specific locations match predefined network topologies connectivity. Despite notable progress neural circuitry engineering, there is still need micropatterned...
Atomic Force Microscopy (AFM) is successfully used for the quantitative investigation of cellular mechanosensing microenvironment. To this purpose, several force spectroscopy approaches aim at measuring adhesive forces between two living cells and also a cell an appropriate reproduction extracellular matrix (ECM), typically exploiting tips suitably functionalised with single components (e.g. collagen, fibronectin) ECM. However, these probes only poorly reproduce complexity native...
Abstract Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC to peritoneum. mechanisms underlying interactions between metastatic cells and ECM, however, remain poorly understood number vitro models available for study peritoneal process is limited. Here, we show that decellularized ECM cavity allows growth organoids obtained PM, favoring development...
In this work, we have validated the application of Hertzian contact mechanics models and corrections in framework linear elasticity for analysis force vs indentation curves acquired using spherical indenters by means finite elements simulations. We systematically investigated impact both large indentations vertical spatial confinement (bottom effect) on accuracy nanomechanical performed with Hertz model parabolic indenter compared to Sneddon indenter.We carried out a comprehensive...
Abstract Biosensors are aimed to detect tiny physical and chemical stimuli in biological systems. Physical forces ubiquitous, being implied all cellular processes, including cell adhesion, migration, differentiation. Given the strong interplay between cells their microenvironment, extracellular matrix (ECM), structural mechanical properties of ECM play an important role transmission external single within tissue. Vice versa, also themselves use self-generated probe biophysical ECM. mechanics...
Although many details remain still elusive, it became increasingly evident in recent years that mechanosensing of microenvironmental biophysical cues and subsequent mechanotransduction are strongly involved the regulation neuronal cell development functioning. This review gives an overview about current understanding brain mechanobiology how impacts on neurogenesis, migration, differentiation, maturation. Therein; we focussing particularly events cell/microenvironment interface decisive...
ABSTRACT Mechanosensing, the ability of cells to perceive and interpret microenvironmental biophysical cues (such as nanotopography), impacts strongly on cellular behaviour through mechanotransductive processes signalling. These events are predominantly mediated by integrins, principal adhesion receptors located at cell/extracellular matrix (ECM) interface. Because typical piconewton force range nanometre length scale interactions, achieving a detailed understanding spatiotemporal dynamics...
ABSTRACT The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many details mechanotransductive signal integration remain elusive due to complexity involved (extra)cellular structures, such as glycocalyx. We used nano-bio-interfaces reproducing complex nanotopographical features extracellular matrix analyse glycocalyx impact on PC12 cell mechanosensing at nanoscale ( e.g ., by force spectroscopy with functionalised probes). Our data...
ABSTRACT The in vitro fabrication of neural networks able to simulate brain circuits and maintain their native connectivity is strategic importance gain a deep understanding circuit physiology natural computational algorithm(s). This would also enable wide-range applications including the development efficient brain-on-chip devices or brain-computer interfaces. Chemical mechanotransductive cues cooperate promote proper functioning networks. Since 80’s, controlled growth mammalian neuronal...
Abstract The crucial role of microtubules in the mitotic-related segregation chromosomes makes them an excellent target for anticancer microtubule targeting drugs (MTDs) such as vinflunine, colchicine, and docetaxel. MTDs affect mitosis by directly perturbing structural organization microtubules. By a direct assessment biomechanical properties prostate cancer cells exposed to different using atomic force microscopy, we show that cell stiffening is candidate mechanism through which preserve...
Atomic force microscopy (AFM) is a powerful tool to investigate interaction forces at the micro and nanoscale. Cantilever stiffness, dimensions geometry of tip can be chosen according requirements specific application, in terms spatial resolution sensitivity. Colloidal probes (CPs), obtained by attaching spherical particle tipless (TL) cantilever, offer several advantages for accurate measurements: tunable well-characterisable radius; higher averaging capabilities (at expense resolution)...
Abstract Peritoneal metastases (PM) are one of the most common routes dissemination for colorectal cancer (CRC) and remain a lethal disease with poor prognosis. The compositional, mechanical structural properties extracellular matrix (ECM) play an important role in development; studying how these change during progression is crucial to understand CRC-PM development. elastic ECMs derived from human samples normal neoplastic PM different pathological conditions were studied by atomic force...
Abstract Atomic Force Microscopy (AFM) is successfully used for the quantitative investigation of cellular mechanosensing microenvironment. To this purpose, several force spectroscopy approaches aim at measuring adhesive forces between two living cells and also a cell suitable reproduction extracellular matrix (ECM), typically exploiting tips suitably functionalised with single components (e.g. collagen, fibronectin) ECM. However, these probes only poorly reproduce complexity native...