A5086761700- DNA Repair Mechanisms
- Nanoplatforms for cancer theranostics
- Carcinogens and Genotoxicity Assessment
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Cancer therapeutics and mechanisms
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- Surface Roughness and Optical Measurements
- Nanoparticles: synthesis and applications
- Inflammatory mediators and NSAID effects
- Cellular transport and secretion
- Medicinal Plant Research
- Bone health and treatments
- interferon and immune responses
- Acute Lymphoblastic Leukemia research
- Polyomavirus and related diseases
- Advanced Nanomaterials in Catalysis
- Pharmacological Effects of Natural Compounds
- Retinoids in leukemia and cellular processes
- Chronic Lymphocytic Leukemia Research
- Bioactive Compounds and Antitumor Agents
- Immune Cell Function and Interaction
Post Graduate Medical Institute
2025
Post Graduate Institute of Medical Education and Research
2018-2024
Institute of Post Graduate Medical Education and Research
2022-2023
IFOM
2017-2021
Institute of Microbial Technology
2021
Rajiv Gandhi Cancer Institute and Research Centre
2018
Indian Institute of Science Bangalore
2010-2017
Abstract Nonhomologous DNA end joining (NHEJ) is one of the major double-strand break (DSB) repair pathways in higher eukaryotes. Recently, it has been shown that alternative NHEJ (A-NHEJ) occurs absence classical and implicated chromosomal translocations leading to cancer. In present study, we have developed a novel biochemical assay system utilizing DSBs flanked by varying lengths microhomology study microhomology-mediated (MMEJ). We show MMEJ can operate normal cells, when present,...
The MRE11-RAD50-NBS1 (MRN) complex supports the synthesis of damage-induced long non-coding RNA (dilncRNA) by polymerase II (RNAPII) from DNA double-strand breaks (DSBs) an unknown mechanism. Here, we show that recombinant human MRN and native RNAPII are sufficient to reconstitute a minimal functional transcriptional apparatus at DSBs. recruits stabilizes Unexpectedly, transcription is promoted independently nuclease activities. Rather, depends on ability melt ends, as shown use mutants...
In the present study, silver (AgNPs) and gold (AuNPs) nanoparticles have been prepared using ethanolic extract of Nepeta leucophylla (NLe). This plant was considered owing to its richness in natural polyphenols antioxidants that are well known for their reducing potential. Different techniques such as UV (Ultraviolet–visible spectrophotometer), FTIR (Fourier transform infrared), XRD (X‐ray diffraction) were utilized characterization nanoparticles. The absorption peak observed at 434 nm 535...
Abstract Polycyclic aromatic molecules such as ellipticine intercalate into double‐stranded DNA and interfere with physiological functions. In the present study, we evaluate chemotherapeutic potential of MPTQ on animal models its mode action. order to test antitumor activity, monohydrochloride was orally administered in mice bearing tumor. Results showed a significant inhibition tumor growth compared that untreated controls. More importantly, mean lifespan animals treated significantly...
Having its genome makes the mitochondrion a unique and semiautonomous organelle within cells. Mammalian mitochondrial DNA (mtDNA) is double-stranded closed circular molecule of about 16 kb coding for 37 genes. Mutations, including deletions in genome, can culminate different human diseases. Mapping deletion junctions suggests that breakpoints are generally seen at hotspots. '9 bp deletion' (8271-8281), intergenic region cytochrome c oxidase II/tRNA
Among various DNA damages, double-strand breaks (DSBs) are considered as most deleterious, they may lead to chromosomal rearrangements and cancer when unrepaired. Nonhomologous end joining (NHEJ) is one of the major DSB repair pathways in higher organisms. A large number studies on NHEJ based vitro systems using cell-free extracts. In this paper, we summarize performed by groups different systems.
Antihyperglycemic action of scopoletin needs to be validated before considering it for clinical trials. The present study explored antihyperglycemic in high-fructose high-fat diet (HFHFD)-induced diabetes rats. animal was performed using 48 rats, 6 each group. HFHFD administered model induction 74 days. Rats Group I (normal control [NC]) and group II (experimental [EC]) received normal saline HFHFD, respectively, throughout the study. Groups III, IV, V, VI oral (1 mg/kg [low dose, LD], 5...
For developing a successful cancer therapeutic modality, the early precise detection of cells in patient biopsies oral squamous cell carcinoma (OSCC) is crucial. This could help researchers create new diagnostic and tools assist clinicians recommending more effective treatment plans improving survival. We have developed an SMPD, cyclooxygenase-2 (COX-2) targeting pH-activable fluorophore named CNP, combining potent COX-2 inhibitor, celecoxib, linked to naphthalimide with acidic...
SUMMARY Having its own genome makes mitochondria a unique and semiautonomous organelle within cells. Mammalian mitochondrial DNA (mtDNA) is double-stranded closed circular molecule of about 16 kb coding 37 genes. Mutations, including deletions in the can culminate different human diseases. Mapping deletion junctions suggests that breakpoints are generally seen at hotspots. ‘9-bp deletion’ (8271-8281), intergenic region cytochrome c oxidase II/tRNA Lys , most common deletion. While it...
Abstract The whole-cell scale spatial organization of lysosomes is regulated by their bidirectional motility on microtubule tracks. Small GTP-binding (G) protein, Arl8b, stimulates the anterograde transport recruiting adaptor protein SKIP (also known as PLEKHM2), which in turn couples motor kinesin-1. Here, we have identified an Arl8b effector, RUN and FYVE domain-containing family member 3, RUFY3, drives retrograde lysosomes. Artificial targeting RUFY3 to surface mitochondria was sufficient...
In the current study, response of EBT3 Films irradiated with a 15 MeV electron beam is analyzed for change in scanning position and orientation within scan area flatbed scanners doses ranging from 50 cGy to 500 read-out data are compared two different i.e., Epson 11000XL 12000XL. The maximum difference obtained OD central quadrant 29%, 13% 15.5%, 22.3%, 7% 9.5%, respectively 12000XL blue, green red channels. Whereas dose 4.1%, 4% 2.5%, 10.1%, 2.5% 6.7%, due film’s orientations direction...
Background: Cigarette smoking is responsible for various lung pathologies including chronic inflammation, emphysema, obstructive pulmonary disease (COPD), cancer, and annually causes almost 10 million deaths globally. During smoke exposure, most affected cells are the alveolar epithelial where as a repair mechanism, activation of cytosolic phospholipase A2 enzymes takes place. High free radicals cPLA2 activity due to continuous exposure leads elevated levels secondary metabolites...