A5011566232- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- PARP inhibition in cancer therapy
- Synthesis and biological activity
- Synthesis and Characterization of Heterocyclic Compounds
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Cell death mechanisms and regulation
- Immune Cell Function and Interaction
- Mitochondrial Function and Pathology
- Tannin, Tannase and Anticancer Activities
- Chronic Lymphocytic Leukemia Research
- Metal complexes synthesis and properties
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Bioinformatics and Genomic Networks
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- COVID-19 Clinical Research Studies
- Polyomavirus and related diseases
- Metabolism, Diabetes, and Cancer
- Phagocytosis and Immune Regulation
The University of Texas MD Anderson Cancer Center
2006-2024
University of Maryland, College Park
2024
Shree Krishna Hospital
2024
Tata Institute of Fundamental Research
2021-2022
Indian Institute of Science Bangalore
2011-2021
TIFR Centre for Interdisciplinary Sciences
2021
IILM Institute for Higher Education
2017
Bangalore University
2015
Brigham and Women's Hospital
2007
MetroHealth
2004
Abstract Naturally occurring compounds are considered as attractive candidates for cancer treatment and prevention. Quercetin ellagic acid naturally flavonoids abundantly seen in several fruits vegetables. In the present study, we evaluate compare antitumor efficacies of quercetin animal models cell lines a comprehensive manner. We found that induced cytotoxicity leukemic cells dose-dependent manner, while showed only limited toxicity. Besides cells, also breast however, its effect on normal...
Abstract Nonhomologous DNA end joining (NHEJ) is one of the major double-strand break (DSB) repair pathways in higher eukaryotes. Recently, it has been shown that alternative NHEJ (A-NHEJ) occurs absence classical and implicated chromosomal translocations leading to cancer. In present study, we have developed a novel biochemical assay system utilizing DSBs flanked by varying lengths microhomology study microhomology-mediated (MMEJ). We show MMEJ can operate normal cells, when present,...
Abstract AMP-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis. Although AMPK has been studied extensively in processes, understanding its substrates and downstream functional network, their contributions to cell fate disease development, remains incomplete. To elucidate the AMPK-dependent signaling pathways, we performed global quantitative phosphoproteomic analysis using wild-type AMPKα1/α2-double knockout cells discovered 160 phosphorylation sites. Further...
Abstract Anticancer drugs, such as camptothecin (CPT), trap topoisomerase I (TOP1) on DNA and form TOP1 cleavage complexes (TOP1cc). Alternative repair pathways have been suggested in the of TOP1cc. However, how these work with TDP1, a key enzyme that specifically hydrolyze covalent bond between catalytic tyrosine 3’-end contribute to TOP1cc is poorly understood. Here, using unbiased whole-genome CRISPR screens generation co-deficient cells TDP1 other genes, we demonstrate MUS81 an important...
Intake of fruits rich in antioxidants daily diet is suggested to be cancer preventive. Sapota a tropical fruit grown and consumed extensively several countries including India Mexico. Here we show that methanolic extracts (MESF) induces cytotoxicity dose-dependent manner cell lines. Cell cycle analysis activation apoptosis, without arresting progression. Annexin V-propidium iodide double-staining demonstrated potentiate apoptosis rather than necrosis cells. Loss mitochondrial membrane...
The t(10;14) translocation involving the HOX11 gene is found in several T-cell leukemia patients. Previous efforts to determine causes of fragility were not successful. role non-B DNA structures increasingly becoming an important cause genomic instability. In present study, bioinformatics analysis revealed two G-quadruplex-forming motifs at breakpoint cluster. Gel shift assays showed formation both intra- and intermolecular G-quadruplexes, latter being more predominant. structure was...
Nonhomologous DNA end joining ( NHEJ ) is the major double‐strand break DSB repair pathway in mammals. Previously, we have described a small molecule inhibitor, SCR 7, which can inhibit Ligase IV ‐dependent manner. Administration of 7 within cells resulted accumulation breaks, cell death, and inhibition tumor growth mice. In present study, report that parental unstable, be autocyclized into stable form. Both cyclized possess same molecular weight (334.09) formula (C 18 H 14 N 4 OS ), whereas...
Eukaryotic cells use copious measures to ensure accurate duplication of the genome. Various genotoxic agents pose threats ongoing replication fork that, if not efficiently dealt with, can result in collapse. It is unknown how precisely controlled and regulated under different conditions. Here, we examined complexity composition upon DNA damage by using a PCNA-based proteomic screen uncover known unexplored players involved stress response. We used camptothecin or UV radiation, which lead...
Recombination activating genes (RAGs), consisting of RAG1 and RAG2, are stringently regulated lymphoid-specific genes, which initiate V(D)J recombination in developing lymphocytes. We report the regulation through a microRNA (miRNA), miR-29c, B cell stage-specific manner mice humans. Various lines experimentation, including CRISPR-Cas9 genome editing, demonstrate target specificity direct interaction miR-29c to RAG1. Modulation levels leads change efficiency pre-B cells. The expression is...
Abstract Polycyclic aromatic molecules such as ellipticine intercalate into double‐stranded DNA and interfere with physiological functions. In the present study, we evaluate chemotherapeutic potential of MPTQ on animal models its mode action. order to test antitumor activity, monohydrochloride was orally administered in mice bearing tumor. Results showed a significant inhibition tumor growth compared that untreated controls. More importantly, mean lifespan animals treated significantly...
5,6-Bis(benzylideneamino)-2-mercaptopyrimidin-4-ol (SCR7) is a new anti cancer molecule having capability to selectively inhibit non-homologous end joining (NHEJ), one of the DNA double strand break (DSB) repair pathways inside cells. In spite promising potential as an anticancer agent, hydrophobicity SCR7 decreases its bioavailability. Herein entrapment in Pluronic copolymer reported. The size aggregates was determined by transmission electron microscopy (TEM) and dynamic light scattering...
The antiapoptotic protein BCL2 is overexpressed in several cancers and contributes to prolonged cell survival chemoresistance, lending itself as an excellent target for cancer therapy. Here, we report the design, synthesis, characterization of Disarib, a novel inhibitor. Disarib showed selective cytotoxicity high lines, CLL patient primary cells, compared low lines. knockdown cells rendered remarkable resistance while sensitivity was regained upon its ectopic expression, establishing...
Type II topoisomerases (TOP2) form transient TOP2 cleavage complexes (TOP2ccs) during their catalytic cycle to relieve topological stress. TOP2ccs are covalently linked TOP2-DNA intermediates that reversible but can be trapped by poisons. Trapped block transactions on DNA and generate genotoxic stress, which the mechanisms of action How cells avoid TOP2cc accumulation remains largely unknown. In this study, we uncovered RAD54 like 2 (RAD54L2) as a key factor mediates TOP2-specific damage...