A5051131946- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- T-cell and B-cell Immunology
- DNA Repair Mechanisms
- Immune Cell Function and Interaction
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Growth Hormone and Insulin-like Growth Factors
- Pharmacogenetics and Drug Metabolism
- Acute Myeloid Leukemia Research
- Constraint Satisfaction and Optimization
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- Phytochemistry and Biological Activities
- Research in Cotton Cultivation
- Plant and Fungal Interactions Research
- Plant Molecular Biology Research
- Cytomegalovirus and herpesvirus research
- FOXO transcription factor regulation
- Genomic variations and chromosomal abnormalities
- RNA modifications and cancer
- Viral Infections and Immunology Research
- Synthesis and Characterization of Heterocyclic Compounds
Indian Institute of Science Education and Research Thiruvananthapuram
2022-2024
Indian Institute of Science Bangalore
2012-2022
NYU Langone Health
2019-2020
New York University
2017
Abstract Naturally occurring compounds are considered as attractive candidates for cancer treatment and prevention. Quercetin ellagic acid naturally flavonoids abundantly seen in several fruits vegetables. In the present study, we evaluate compare antitumor efficacies of quercetin animal models cell lines a comprehensive manner. We found that induced cytotoxicity leukemic cells dose-dependent manner, while showed only limited toxicity. Besides cells, also breast however, its effect on normal...
Abstract Background Ubiquitously expressed CTCF is involved in numerous cellular functions, such as organizing chromatin into TAD structures. In contrast, its paralog, CTCFL, normally only present the testis. However, it also aberrantly many cancers. While known that shared and unique zinc finger sequences CTCFL enable to bind competitively a subset of binding sites well own locations, impact on chromosome organization gene expression has not been comprehensively analyzed context function....
Chromosomal translocations are considered as one of the major causes lymphoid cancers. RAG complex, which is responsible for V(D)J recombination, can also cleave non-B DNA structures and cryptic RSSs in genome leading to chromosomal translocations. The mechanism factors regulating illegitimate function RAGs resulting oncogenesis largely unknown. Upon silico analysis 3760 from cancer patients, we find that 93% translocation breakpoints possess adjacent nonamers (RAG binding sequences), 77%...
Abstract Integrase inhibitors are a class of antiretroviral drugs used for the treatment AIDS that target HIV integrase, an enzyme responsible integration viral cDNA into host genome. RAG1, critical involved in V(D)J recombination exhibits structural similarity to integrase. We find two integrase inhibitors, Raltegravir and Elvitegravir, interfered with physiological functions RAGs such as binding, cleavage hairpin formation at signal sequence (RSS), though effect was limited. Circular...
The RAG (recombination-activating gene) complex is responsible for the generation of antigen receptor diversity by acting as a sequence-specific nuclease. Recent studies have shown that it also acts structure-specific However, little known about factors regulating this activity at genomic level. We show in present study proximity V(D)J nonamer to heteroduplex DNA significantly increases cleavage and binding efficiencies physiological concentrations MgCl(2). position with respect was...
Acute myeloid leukemia (AML) is one of the leading leukemic malignancies in adults. The heterogeneity disease makes diagnosis and treatment extremely difficult. With advent next-generation sequencing (NGS) technologies, exploration at molecular level for identification biomarkers drug targets has been focus researchers to come up with novel therapies better prognosis survival outcomes AML patients. However, huge amount data from NGS platforms requires a comprehensive platform streamline...
Summary The function of the CCCTC-binding factor (CTCF) in organization genome has become an important area investigation, but mechanisms how CTCF dynamically contributes to is not clear. We previously discovered that binds large numbers endogenous RNAs; promoting its oligomerization. Here we found inhibition transcription or interfering with ability bind RNA through mutations two 11 zinc fingers are involved binding cognate site vitro, finger-1 (ZF1) −10 (ZF10), disrupt association...
Many DNA lesions associated with lymphoid malignancies are linked to off-target cleavage by the RAG1/2 recombinase. However, has mostly been analyzed in context of repair defects, confounding any mechanistic understanding deregulation. We identified a conserved SQ phosphorylation site on RAG2 365 366 that is involved feedback control RAG cleavage. Mutation serine non-phosphorylatable alanine permits bi-allelic and bi-locus RAG-mediated breaks same cell, leading reciprocal translocations....
Background Ubiquitously expressed CTCF is involved in numerous cellular functions, such as organizing chromatin into TAD structures. In contrast, its paralog, CTCFL normally only present testis. However, it also aberrantly many cancers. While known that shared and unique zinc finger sequences enable to bind competitively a subset of binding sites well own locations, the impact on chromosome organization gene expression has not been comprehensively analyzed context function. Using an...
Recombination activating genes (RAGs), consisting of RAG1 and RAG2 have ability to perform spatially temporally regulated DNA recombination in a sequence specific manner. Besides, RAGs also cleave at non-B structures are thought contribute towards genomic rearrangements cancer. The nonamer binding domain binds the signal during V(D)J recombination. However, deletion NBD did not affect RAG cleavage on structures. In present study, we investigated involvement other domains when act as...
Abstract Acute myeloid leukemia (AML) is one of the leading leukemic malignancies in adults. The heterogeneity disease makes diagnosis and treatment extremely difficult. Despite significant developments rapid advancements finding new medication targets, for AML remains a challenge. With advent next-generation sequencing (NGS) technologies, exploration at molecular level identification biomarkers drug targets has been main focus researchers to come up with novel therapies better prognosis...