A5061181543- Genomics and Chromatin Dynamics
- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Acute Lymphoblastic Leukemia research
- Protein Degradation and Inhibitors
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- Chromosomal and Genetic Variations
- Cancer Immunotherapy and Biomarkers
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- Immune cells in cancer
- Kruppel-like factors research
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Lymphoma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- MicroRNA in disease regulation
Helmholtz Centre for Infection Research
2016-2024
New York University
2017-2023
NYU Langone Health
2017-2023
Heinrich Heine University Düsseldorf
2015-2023
Merck (Germany)
2023
Weill Cornell Medicine
2021
NYU Langone’s Laura and Isaac Perlmutter Cancer Center
2019-2021
Cornell University
2021
Düsseldorf University Hospital
2020
German Cancer Research Center
2019
T-DNA insertion mutants are very valuable for reverse genetics in Arabidopsis thaliana. Several projects have generated large sequence-indexed collections of lines, which GABI-Kat is the second largest resource worldwide. User access to collection and its Flanking Sequence Tags (FSTs) provided by front end SimpleSearch (http://www.GABI-Kat.de). significant improvements been implemented recently. The database now relies on TAIRv10 genome sequence annotation dataset. All FSTs newly mapped...
Abstract The BCL2 family plays important roles in acute myeloid leukemia (AML). Venetoclax, a selective inhibitor, has received FDA approval for the treatment of AML. However, drug resistance ensues after prolonged treatment, highlighting need greater understanding underlying mechanisms. Using genome-wide CRISPR/Cas9 screen human AML, we identified genes whose inactivation sensitizes AML blasts to venetoclax. Genes involved mitochondrial organization and function were significantly depleted...
GCN2 is a key driver of Mϕ and MDSC polarization in the tumor microenvironment causing T cell exhaustion.
The manipulation of T cell metabolism to enhance anti-tumor activity is an area active investigation. Here, we report that activating the amino acid starvation response in effector CD8+ cells ex vivo using general control non-depressible 2 (GCN2) agonist halofuginone (halo) enhances oxidative and function. Mechanistically, identified autophagy coupled with CD98-mTOR axis as key downstream mediators phenotype induced by halo treatment. adoptive transfer halo-treated into tumor-bearing mice...
Innate immune activation is essential to mount an effective antiviral response and prime adaptive immunity. Although a crucial role of CD169+ cells during vesicular stomatitis virus (VSV) infections increasingly recognized, factors regulating viral remain unclear. Here, we show that tumor necrosis factor produced by CD11b+ Ly6C+ Ly6G+ following infection with VSV. The absence TNF or receptor 1 (TNFR1) resulted in reduced numbers type I interferon (IFN-I) production VSV infection, severe...
CTCF and cohesin play a key role in organizing chromatin into topologically associating domain (TAD) structures. Disruption of single binding site is sufficient to change chromosomal interactions leading alterations modifications gene regulation. However, the extent which can disrupt 3D chromosome organization transcriptional changes unknown. In multiple myeloma, 4;14 translocation induces overexpression histone methyltransferase, NSD2, resulting expansion H3K36me2 shrinkage antagonistic...
Abstract Background Ubiquitously expressed CTCF is involved in numerous cellular functions, such as organizing chromatin into TAD structures. In contrast, its paralog, CTCFL, normally only present the testis. However, it also aberrantly many cancers. While known that shared and unique zinc finger sequences CTCFL enable to bind competitively a subset of binding sites well own locations, impact on chromosome organization gene expression has not been comprehensively analyzed context function....
MicroRNA are well-established players in post-transcriptional gene regulation. However, information on the effects of microRNA deregulation mainly relies bioinformatic prediction potential targets, whereas proof direct physical microRNA/target messenger RNA interaction is mostly lacking. Within International Cancer Genome Consortium Project "Determining Molecular Mechanisms Malignant Lymphoma by Sequencing", we performed miRnome sequencing from 16 Burkitt lymphomas, 19 diffuse large B-cell...
Abstract Influenza during pregnancy can affect the health of offspring in later life, among which neurocognitive disorders are best described. Here, we investigate whether maternal influenza infection has adverse effects on immune responses offspring. We establish a two-hit mouse model to study effect A virus (first hit) vulnerability heterologous infections (second life. Offspring born infected mothers stunted growth and more vulnerable (influenza B MRSA) than those PBS- or...
The RNA-binding protein Musashi 2 (MSI2) has emerged as an important regulator in cancer initiation, progression, and drug resistance. Translocations deregulation of the MSI2 gene are diagnostic certain cancers, including chronic myeloid leukemia (CML) with translocation t(7;17), acute (AML) t(10;17), some cases B-precursor lymphoblastic (pB-ALL). To better understand function leukemia, mRNA targets that bound regulated by their MSI2-binding motifs need to be identified. this end, using...
Abstract IDH1/2 mutations are early drivers present in diverse human cancer types arising various tissue sites. mutation is known to induce a global hypermethylator phenotype. However, the effects on DNA methylation across IDH mutant cancers and functionally different genome regions, remain unknown. We analyzed data from acute myeloid leukemia, oligodendroglioma, astrocytoma, solid papillary breast carcinoma with reverse polarity, sinonasal undifferentiated cholangiocarcinoma, which...
Abstract Immune checkpoint inhibitors (ICI), which target immune regulatory pathways to unleash antitumor responses, have revolutionized cancer immunotherapy. Despite the remarkable success of ICI immunotherapy, a significant proportion patients whose tumors respond these treatments develop immune-related adverse events (irAE) resembling autoimmune diseases. Although clinical spectrum irAEs is well characterized, their successful management remains empiric. This in part because pathogenic...
B-cell epitopes (BCEs) play a pivotal role in the development of peptide vaccines, immuno-diagnostic reagents and antibody production, thus infectious disease prevention diagnostics general. Experimental methods used to determine BCEs are costly time-consuming. Therefore, it is essential develop computational for rapid identification BCEs. Although several have been developed this task, generalizability still major concern, where cross-testing classifiers trained tested on different datasets...