A5053189786- Tryptophan and brain disorders
- Cancer, Stress, Anesthesia, and Immune Response
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Toxoplasma gondii Research Studies
- Cancer Immunotherapy and Biomarkers
- Prostate Cancer Treatment and Research
- Cytomegalovirus and herpesvirus research
- Telomeres, Telomerase, and Senescence
- RNA modifications and cancer
- Parasitic Infections and Diagnostics
- Genomics and Chromatin Dynamics
- Colorectal Cancer Treatments and Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Pluripotent Stem Cells Research
- Cancer Research and Treatments
- Herpesvirus Infections and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Histone Deacetylase Inhibitors Research
The University of Texas MD Anderson Cancer Center
2019-2024
The University of Texas Health Science Center at Houston
2022
Weill Cornell Medicine
2017-2021
Cornell University
2017-2021
Lanzhou Veterinary Research Institute
2019-2020
Chinese Academy of Agricultural Sciences
2019-2020
South China Agricultural University
2018-2019
Donghua University
2003
Tsinghua University
2003
Oncogenic KRAS (KRAS*) is a key tumor maintenance gene in pancreatic ductal adenocarcinoma (PDAC), motivating pharmacologic targeting of KRAS* and its effectors. Here, we explored mechanisms involving the microenvironment (TME) as potential basis for resistance to KRAS*. Using inducible Kras G12D;Trp53 -/- PDAC mouse model, gain-of-function screens epigenetic regulators identified HDAC5 top hit enabling independent growth. HDAC5-driven escaper tumors showed prominent neutrophil-to-macrophage...
Abstract Genetic inactivation of PTEN is common in prostate cancer and correlates with poorer prognosis. We previously identified CHD1 as an essential gene PTEN-deficient cells. Here, we sought definitive vivo genetic evidence for, mechanistic understanding of, the role cancer. In Pten Pten/Smad4 genetically engineered mouse models, prostate-specific deletion Chd1 resulted markedly delayed tumor progression prolonged survival. was associated profound microenvironment (TME) remodeling...
Tumor-associated macrophages (TAMs) are key regulators of the complex interplay between cancer and immune microenvironment. Tumor cell-derived spondin 2 (SPON2) is an extracellular matrix glycoprotein that has complicated roles in recruitment neutrophils during inflammation. Overexpression SPON2 been shown to promote tumor cell migration colorectal (CRC). However, mechanism by which regulates accumulation TAMs microenvironment (TME) CRC unknown.Immunohistochemistry was used examine...
Inactivation of adenomatous polyposis coli (APC) is common across many cancer types and serves as a critical initiating event in most sporadic colorectal cancers. APC deficiency activates WNT signaling, which remains an elusive target for therapy, prompting us to apply the synthetic essentiality framework identify druggable vulnerabilities APC-deficient Tryptophan 2,3-dioxygenase 2 (TDO2) was identified essential effector cancer. Mechanistically, results TCF4/β-catenin-mediated upregulation...
Abstract Germline telomere maintenance defects are associated with an increased incidence of inflammatory diseases in humans, yet whether and how dysfunction causes inflammation not known. Here, we show that drives pATM/c-ABL-mediated activation the YAP1 transcription factor, up-regulating major pro-inflammatory pro-IL-18. The colonic microbiome stimulates cytosolic receptors activating caspase-1 which cleaves pro-IL-18 into mature IL-18, leading to recruitment interferon (IFN)-γ-secreting T...
The interferon (IFN) response is vital for the effectiveness of immune checkpoint inhibition (ICI) therapy. Our previous research showed that KRAS (Kirsten rat sarcoma viral) mutation impairs IFN in colorectal cancer (CRC), with an unclear mechanism. Here, we demonstrate accelerates double-stranded RNA (dsRNA) degradation, impairing dsRNA sensing and by down-regulating DExD/H-box helicase 6 (DDX60). DDX60 was identified as a target here could bind to dsRNAs protect against RNA-induced...
Cathepsin B, a lysosomal cysteine protease, is thought to be involved the pathogenesis of Fasciola gigantica infection, but its exact role remains unclear. In present study, recombinant F. cathepsin B (rFgCatB) protein was expressed in methylotrophic yeast Pichia pastoris. Western blot analysis confirmed reactivity purified rFgCatB serum from gigantica-infected goats. The effects serial concentrations (10, 20, 40, 80, and 160 μg/ml) on various functions goat peripheral blood mononuclear...
We characterized the porcine tissue transcriptional landscapes that follow Toxoplasma gondii infection. RNAs were isolated from liver, spleen, cerebral cortex, lung, and mesenteric lymph nodes (MLNs) of T. gondii-infected uninfected (control) pigs at days 6 18 postinfection, analyzed using next-generation sequencing (RNA-seq). altered expression 178, 476, 199, 201, 362 transcripts dpi 217, 223, 347, 119, 161 in infected brain, MLNs respectively. The differentially expressed (DETs) grouped...
Toxoplasma gondii is a leading cause of foodborne illness and consumption undercooked pig meat major risk factor for acquiring toxoplasmosis, which causes substantial burden on society. Here, we used isobaric tags relative absolute quantification (iTRAQ) labelling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify cellular proteins pathways altered during T. infection in pigs. We also parallel reaction monitoring-based LC-MS/MS verify the levels protein...
Toxoplasma gondii secretes a number of virulence-related effector proteins, such as the rhoptry protein 18 (ROP18). To further broaden our understanding molecular functions ROP18, we examined transcriptional response human embryonic kidney cells (HEK293T) to ROP18 type I T. RH strain. Using RNA-sequencing, compared transcriptome ROP18-expressing HEK293T control cells. Our analysis revealed that altered expression 750 genes (467 upregulated and 283 downregulated genes) in Gene ontology (GO)...
Significance Prostate cancer is a major cause of cancer-related death in men. Emerging resistance to androgen receptor (AR) signaling inhibition therapy has heightened the need identify therapeutic vulnerabilities specific AR-negative prostate cancers. Analysis CRISPR screen datasets human cells identified high dependency for histone demethylase JMJD1C across multiple model systems. Integrated analyses revealed that combined AR and inactivation drives up-regulation TNFα network which...
Infection with the apicomplexan protozoan parasite T. gondii can cause severe and potentially fatal cerebral ocular disease, especially in immunocompromised individuals. The anticoccidial ionophore drug monensin has been shown to have anti-Toxoplasma properties. However, comprehensive molecular mechanisms that underlie effect of on are still largely unknown. We hypothesized analysis transcriptional changes induced by treatment reveal new aspects mechanism action against gondii. Porcine...
ABSTRACT The identity of dividing cells is challenged during mitosis, as transcription halted and chromatin architecture drastically altered. How cell type-specific gene expression genomic organization are faithfully reset upon G1 entry in daughter remains elusive. To address this issue, we characterized at a genome-wide scale the dynamic transcriptional architectural resetting mouse pluripotent stem (PSCs) mitotic exit. This revealed distinct patterns reactivation with rapid induction genes...