A5040526199- MicroRNA in disease regulation
- Lung Cancer Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Esophageal Cancer Research and Treatment
- CAR-T cell therapy research
- Neuroscience of respiration and sleep
- Acute Myeloid Leukemia Research
- Sulfur Compounds in Biology
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
- Neonatal Respiratory Health Research
- Alzheimer's disease research and treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer-related molecular mechanisms research
- Melanoma and MAPK Pathways
- Lung Cancer Treatments and Mutations
- Immunotherapy and Immune Responses
- Neonatal Health and Biochemistry
- Animal Nutrition and Physiology
- Protein Degradation and Inhibitors
- Virus-based gene therapy research
- Osteoarthritis Treatment and Mechanisms
- RNA modifications and cancer
Dalian Medical University
2021-2025
Chongqing Medical University
2021-2025
Second Affiliated Hospital of Chongqing Medical University
2021-2025
New York University
2017-2024
NYU Langone Health
2017-2024
Jiangxi Agricultural University
2022-2023
Promega (United States)
2017-2023
Shuguang Hospital
2022
Shanghai University of Traditional Chinese Medicine
2022
Merck & Co., Inc., Rahway, NJ, USA (United States)
2007-2021
We undertook a comprehensive proteogenomic characterization of 95 prospectively collected endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis revealed possible new consequences perturbations to the p53 Wnt/β-catenin pathways, identified potential role for circRNAs in epithelial-mesenchymal transition, provided information about proteomic markers clinical genomic tumor subgroups, including relationships known druggable pathways. An extensive genome-wide...
Inheritance of the apoE4 allele (ε4) increases risk developing Alzheimer's disease; however, mechanisms underlying this association remain elusive. Recent data suggest that inheritance ε4 may lead to reduced apoE protein levels in CNS. We therefore examined brains, CSF and plasma ε2/2, ε3/3, ε4/4 targeted replacement mice. These mice showed a genotype-dependent decrease levels; ε2/2 >ε3/3 >ε4/4. Next, we sought examine relative contributions apoE3 ε3/4 mouse brains. ApoE4 represented...
Abstract The BCL2 family plays important roles in acute myeloid leukemia (AML). Venetoclax, a selective inhibitor, has received FDA approval for the treatment of AML. However, drug resistance ensues after prolonged treatment, highlighting need greater understanding underlying mechanisms. Using genome-wide CRISPR/Cas9 screen human AML, we identified genes whose inactivation sensitizes AML blasts to venetoclax. Genes involved mitochondrial organization and function were significantly depleted...
Abstract Deregulation of the BCL-2 family interaction network ensures cancer resistance to apoptosis and is a major challenge current treatments. Cancer cells commonly evade through upregulation anti-apoptotic proteins; however, more resistant cancers also downregulate or inactivate pro-apoptotic proteins suppress apoptosis. Here, we find that in diverse panel solid hematological malignancies mediated by both overexpression BCL-XL an unprimed apoptotic state, limiting direct indirect...
Transgenic mice (Tg2576) overexpressing the Swedish mutation of human amyloid precursor protein display biochemical, pathological, and behavioral markers consistent with many aspects Alzheimer's disease, including impaired hippocampal function. Impaired, hippocampal-dependent, contextual fear conditioning (CFC) is observed in as young 20 weeks age. This impairment can be attenuated after treatment before training phosphodiesterase-4 inhibitor rolipram (0.1 mg/kg, i.p.). A rolipram-associated...
γ-Secretase is a membrane protein complex that cleaves the β-amyloid precursor (APP) within transmembrane region, after prior processing by β-secretase, producing amyloid β-peptides Aβ 40 and 42 . Errant production of Aβ-peptides substantially increases has been associated with formation plaques in Alzheimer's disease patients. Biophysical genetic studies indicate presenilin-1, which contains proteolytic active site, three other proteins [nicastrin, anterior pharynx defective-1 (APH-1),...
Immune checkpoint inhibitors (anti-CTLA-4, anti-PD-1, or the combination) enhance anti-tumor immune responses, yielding durable clinical benefit in several cancer types, including melanoma. However, a subset of patients experience immune-related adverse events (irAEs), which can be severe and result treatment termination. To date, no biomarker exists that predict development irAEs. We hypothesized pre-treatment antibody profiles identify who possess sub-clinical autoimmune phenotype...
N-acetylserotonin (NAS) is an immediate precursor of melatonin, which we have reported neuroprotective against ischemic injury. Here test whether NAS a potential agent in experimental models We demonstrate that inhibits cell death induced by oxygen-glucose deprivation or H2O2 primary cerebrocortical neurons and hippocampal vitro, organotypic slice cultures ex vivo reduces hypoxia/ischemia injury the middle cerebral artery occlusion mouse model ischemia vivo. find inhibiting mitochondrial...
Abstract Increased intestinal permeability and translocation of gut bacteria trigger various polyaetiological diseases associated with chronic inflammation underlie a variety poorly treatable pathologies. Previous studies have established primary role the microbiota composition in such Using rat model, we examined effects exposure to bacteriophage cocktail on relative abundance taxonomic units bacterial community. There was an increase markers impaired permeability, as lactulose/mannitol...
Abstract BH3 mimetics are used as an efficient strategy to induce cell death in several blood malignancies, including acute myeloid leukemia (AML). Venetoclax, a potent BCL-2 antagonist, is clinically combination with hypomethylating agents for the treatment of AML. Moreover, MCL1 or dual BCL-2/BCL-xL antagonists under investigation. Yet, resistance single combinatorial BH3-mimetic therapies eventually ensues. Integration multiple genome-wide CRISPR/Cas9 screens revealed that loss mitophagy...
Natural killer (NK) cells are cytotoxic lymphocytes that critical for the innate immune system. Engineering NK with chimeric antigen receptors (CARs) allows CAR-NK to target tumor antigens more effectively. In this report, we present novel CAR mRNA-LNP (lipid nanoparticle) technology effectively transfect expanded from primary PBMCs and generate functional cells. CD19-CAR mRNA BCMA-CAR were embedded into LNPs resulted in 78% 95% expression cells, respectively. BCMA-CAR-NK after transfection...
CD47 is a glycoprotein of the immunoglobulin superfamily that often overexpressed in different types hematological and solid cancer tumors plays important role blocking phagocytosis, increased tumor survival, metastasis angiogenesis. In present report, we designed CAR (chimeric antigen receptor)-T cells bind antigen. We used ScFv (single chain variable fragment) from mouse antibody to generate CD47-CAR-T for targeting cell lines. effectively killed ovarian, pancreatic other produced high...
Hypoxia is a major characteristic of the solid tumor microenvironment. To understand how chimeric antigen receptor-T cells (CAR-T cells) function in hypoxic conditions, we characterized CD19-specific and BCMA-specific human CAR-T generated atmospheric (18% oxygen) (1% culture for expansion, differentiation status, CD4:CD8 ratio. expanded to much lower extent 1% oxygen than 18% oxygen. Hypoxic also had less differentiated phenotype higher ratio cells. were then added antigen-positive...
The pathogenesis of osteoarthritis (OA) includes both mechanical and inflammatory features. Studies have implicated synovial fluid uric acid (UA) as a potential OA biomarker, possibly reflecting chondrocyte damage. Whether serum UA levels reflect/contribute to is unknown. We investigated whether predict progression in non-gout knee population.Eighty-eight patients with medial (body mass index [BMI] <33 kg/m2 ) but without gout were studied. Baseline measured previously banked samples. At 0...
This study tested the hypothesis that glycine improves intestinal barrier function through regulating oxidative stress in broilers exposed to heat stress. A total of 300 twenty-one-day-old female Arbor Acres (600 ± 2.5g) was randomly allocated 5 treatments (6 replicate 10 birds each). The were as follows: control group (CON) kept under thermoneutral condition (24 1°C) and fed a basal diet. Broilers diet reared high ambient temperature (HT) considered HT (34 1°C for 8 h/d). supplemented with...
The epithelial cell adhesion molecule (EpCAM) is often overexpressed in many types of tumors, including colorectal cancer. We sequenced and humanized an EpCAM mouse antibody used it to develop bispecific EpCAM-CD3 antibodies. Three different designs were generate antibodies such as CrossMab knob-in-hole, ScFv-CD3 ScFv (BITE), ScFv-human Fc designs. These showed strong specific binding the EpCAM-positive Lovo line T cells, specifically killed cells not EpCAM-negative Colo741 presence...
Neuropsychiatric symptoms of systemic lupus erythematosus (NP-SLE) have been understudied compared to end-organ failure and peripheral pathology. symptoms, particularly affective cognitive indications, may be among the earliest manifestations SLE. Among potential pathophysiological mechanisms responsible for NP-SLE are increased pro-inflammatory cytokines, subsequent induction indoleamine-2,3-dioxygenase (IDO) activation kynurenine pathway. In MRL/MpJ-Faslpr (MRL/lpr) murine model lupus,...
Objective In these studies, we examined the association of single nucleotide polymorphisms (SNPs) IL1RN gene with radiographic severity symptomatic knee osteoarthritis (SKOA) and risk incident OA. We also explored genetic in patients new onset rheumatoid arthritis (RA). Methods Over 1000 subjects who met American College Rheumatology criteria for tibiofemoral OA were selected from three independent, National Institute Health (NIH)-funded cohorts. CTA TTG haplotypes formed SNPs (rs419598,...
Chimeric antigen receptor (CAR) T cell therapy has emerged as a pioneering cancer treatment, achieving unprecedented success in treating certain hematological malignancies such lymphomas and leukemias. However, more patients receive CAR-T therapies, treatment-associated secondary primary are increasingly being reported partly due to unexpected CAR transgene insertion, raising serious safety concerns. To address this issue, we describe here nonviral, non-integrating approach generate...