The role of extracellular DNA in the maintenance biofilms formed by gram-positive and gram-negative bacteria was studied. This study evaluated all bacterial strains that were tested for presence with an average size 30 kb matrix. Our results indicate changes community biomass, architecture, morphology, numbers CFU DNase. effect seems to be common established various unrelated bacteria. cleavage leads formation altered biofilm permits increased penetration antibiotics. Thus, addition DNase...

Recent studies suggest that alterations in the gut phagobiota may contribute to pathophysiological processes mammals; however, association of bacteriophage community structure with Parkinson's disease (PD) has not been yet characterized. Towards this end, we used a published dataset analyse composition and determine phage/bacteria ratio faecal samples from drug-naive PD patients healthy participants. Our analyses revealed significant representation certain bacteriophages patients. We...

Abstract Increased intestinal permeability and translocation of gut bacteria trigger various polyaetiological diseases associated with chronic inflammation underlie a variety poorly treatable pathologies. Previous studies have established primary role the microbiota composition in such Using rat model, we examined effects exposure to bacteriophage cocktail on relative abundance taxonomic units bacterial community. There was an increase markers impaired permeability, as lactulose/mannitol...

Currently, the world is struggling with coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Prions are proteins that possess a unique conformational conversion, ability to rapidly shift between multiple conformations due residue hydrophobicity and net sequence charge, viral prion-like known as potential regulators of infections. However, domains (PrD) in SARS-CoV-2 proteome have not been analyzed. In this silico study, using PLAAC...

Biofilm formation plays a crucial role in the development of different infections. This study was designed to examine effects extracellular DNA destruction by DNase I on characteristics forming bacterial biofilms. We have found that matrix biofilms formed presence contains fragments about 30 kb. These data support idea cell-free is constantly released Our results indicate an important properties Biofilms (5.0 μg/mL) displayed reduced biofilm biomass, total decreased viability bacteria, and...

Increased intestinal permeability and translocation of gut microbiota from the lumen to systemic circulation predispose patients various diseases may be one main triggers thereof. The role in increased is under intensive investigation. Here, we studied alterations host as a direct effect treatment with bacteriophage cocktail. After 10 days challenge, rats showed weight loss, messy hair, decreased activity. Additionally, they displayed significantly elevated lactulose:mannitol ratio level...

Abstract The etiopathogenesis of type 1 diabetes (T1D), a common autoimmune disorder, is not completely understood. Recent studies suggested the gut microbiome plays role in T1D. We have used public longitudinal data from T1D patients to analyze amyloid-producing bacterial composition and found significant association between initially high Escherichia coli abundance, subsequent E . depletion prior seroconversion, development. In children who presented seroconversion or developed T1D, we...

The pathogenesis of numerous human multifaceted devastating diseases, including a variety neurodegenerative and autoimmune is associated with alterations in the gut microbiota; however, underlying mechanisms are not completely understood. Our recent metagenome phagobiota proteome analyses studies relevant animal models suggested that bacterial viruses might be implicated progression maintenance at least some pathologies, those protein misfolding. Here, for first time, we propose concept...

Liver metastasis is the main cause of colorectal cancer (CRC)‐related death. Neutrophil extracellular traps (NETs) play important roles in CRC progression. Deoxyribonuclease I (DNase I) has been shown to alter NET function by cleaving DNA strands comprising backbone. Moreover, DNase displays high antimetastatic activity multiple tumor models. To circumvent long‐term daily administrations recombinant I, we have developed an adeno‐associated virus (AAV) gene therapy vector specifically express...

Unrelated spore-forming bacteria share unique characteristics stemming from the presence of highly resistant endospores, leading to similar challenges in health and disease. These are related these transmissible spores, which commonly spread within environment implicated host-to-host transmission. In humans, contribute a variety pathological processes that characteristics, including persistence, chronicity, relapses maintenance resistome. We first outline necessity characterizing totality as...

Abstract A hallmark feature of Alzheimer’s disease (AD) and other tauopathies is the misfolding, aggregation cerebral accumulation tau deposits. Compelling evidence indicates that misfolded aggregates are neurotoxic, producing synaptic loss neuronal damage. Misfolded able to spread pathology from cell-to-cell by a prion like seeding mechanism. The factors implicated in initiation progression misfolding largely unclear. In this study, we evaluated effect DNA extracted diverse prokaryotic...

Abstract Prions are proteins that can self-propagate, leading to the misfolding of proteins. In addition previously demonstrated pathogenic roles prions during development different mammalian diseases, including neurodegenerative they have recently been shown represent an important functional component in many prokaryotic and eukaryotic organisms bacteriophages, confirming unexplored regulatory roles. However, in-depth analysis these domains viruses has not performed. Here, we examined...

Prions are molecules characterized by self-propagation, which can undergo a conformational switch leading to the creation of new prions. Prion proteins have originally been associated with development mammalian pathologies; however, recently they shown contribute environmental adaptation in variety prokaryotic and eukaryotic organisms. Bacteriophages widespread represent important regulators microbiota homeostasis be diverse across various bacterial families. Here, we examined whether...

Alzheimer’s disease is associated with prion-like aggregation of the amyloid β (Aβ) peptide and subsequent accumulation misfolded neurotoxic aggregates in brain. Therefore, it critical to clearly identify factors that trigger cascade Aβ misfolding aggregation. Numerous studies have pointed out association between microorganisms their virulence disease; however, exact mechanisms action remain unclear. Recently, we discovered a new pathogenic role bacterial extracellular DNA, triggering...

Our previous studies revealed the existence of a Universal Receptive System that regulates interactions between cells and their environment. This system is composed DNA- RNA-based Teazeled receptors (TezRs) found on surface prokaryotic eukaryotic cells, as well integrases recombinases. In current study, we aimed to provide further insight into regulatory role TezR its loss in Staphylococcus aureus gene transcription. To this end, transcriptomic analysis S. MSSA VT209 was performed following...

Abstract The understanding of the mechanisms that control key features immune cells in various disease contexts remains limited, and few techniques are available for manipulating cells. Thus, discovering novel strategies regulating is essential gaining insight into their roles health disease. In this study, we investigated potential recently described Universal Receptive System to regulate human cell functions. This was achieved first time by specifically targeting newly discovered...

Medical treatment for tuberculosis is complicated nowadays by the appearance of new multiresistant strains, and therefore, antibiotics are in great need. Here, we report synthesis vitro testing a class highly selective antimicrobial boron‐containing peptidomimetics with compounds exhibiting activity against Mycobacterium at ≤5 μg/mL. The approach developed makes it possible to synthesize variously substituted β‐aminoboronic acids their derivatives high level diastereoselectivity.

Bacterial directional migration plays a significant role in bacterial adaptation. However, the regulation of this process, particularly young biofilms, remains unclear. Here, we demonstrated critical extracellular RNA as part Universal Receptive System using multidisciplinary approach, including culture, biochemistry, and genetics. We found that destruction or inactivation with RNase RNA-specific antibodies presence chemoattractant triggered formation "runner cells» what call "panic state"...

Tuberculosis is still affecting millions of people worldwide, and new resistant strains Mycobacterium tuberculosis are being found. It therefore necessary to find compounds for treatment. In this paper, we report the synthesis in vitro testing peptidyl β-aminoboronic acids β-aminoboronates with anti-tubercular activity.

Abstract Here we have proposed a new biological definition of life based on the function and reproduction existing genes creation ones, which is applicable to both unicellular multicellular organisms. First, coined term “genetic information metabolism” comprising functioning, reproduction, their distribution among living non-living carriers genetic information. Encompassing this concept, defined as organized matter that provides metabolism. Additionally, articulated general Tetz law:...

The present study describes a previously unknown universal system that orchestrates the interaction of bacteria with environment, named Teazeled receptor (TR-system). identical was recently discovered within eukaryotes. includes DNA- and RNA-based molecules "TezRs", form receptor's network located outside membrane, as well reverse transcriptases integrases. TR-system takes part in control all major aspects bacterial behavior, such intra cellular communication, growth, biofilm formation...

Here, our data provide the first evidence for existence of a previously unknown receptive system formed by novel DNA- and RNA-based receptors in eukaryotes. This system, named TR-system, is capable recognizing generating response to different environmental factors has been shown orchestrate major vital functions fungi, mammalian cells, plants. Recently, we discovered similar regulatory prokaryotes. These are localized outside membrane forming type network around cells that responds variety...

Alzheimer's disease is the most common cause of dementia and characterized by a progressive loss brain tissue leading to amyloid-β accumulation severe decline in cognitive function. The poorly understood, available treatments are limited their efficacy, particularly for patients with more symptoms. We report case 77-year-old Caucasian man behavioral disturbance secondary treated memantine who began adjunct treatment deoxyribonuclease I. Prior initiation I treatment, our patient appeared be...

The antimicrobial activity of Mul-1867, a novel synthetic compound, was tested against 18 bacterial strains, including clinical isolates and reference strains from culture collections.The minimal inhibitory concentration (MICs) bactericidal (MBCs) were determined by using the broth macrodilution method. kinetics effects Mul-1867 biofilm-growing microorganisms assessed at time-kill test in vitro 48-h-old biofilms Staphylococcus aureus Escherichia coli. Transmission electron microscopy...