A5008254848- Immune cells in cancer
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nanoplatforms for cancer theranostics
- Prostate Cancer Treatment and Research
- Immune Response and Inflammation
- Organ Transplantation Techniques and Outcomes
- DNA and Nucleic Acid Chemistry
- Liver Disease Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- DNA Repair Mechanisms
- Advanced Glycation End Products research
- Liver Disease and Transplantation
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Hepatocellular Carcinoma Treatment and Prognosis
- Liver physiology and pathology
- Cancer, Lipids, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Inflammatory Biomarkers in Disease Prognosis
- Epigenetics and DNA Methylation
- Nitric Oxide and Endothelin Effects
Anshan Hospital
2021-2025
Guangdong Medical College
2021-2025
Huazhong University of Science and Technology
2015-2025
Tongji Hospital
2015-2025
The Ohio State University
2020-2025
Shuguang Hospital
2025
Shanghai University of Traditional Chinese Medicine
2022-2025
Guangzhou University of Chinese Medicine
2024
Hangzhou Hospital of Traditional Chinese Medicine
2024
Feinstein Institute for Medical Research
2022-2024
Abstract Risks of tumor recurrence after surgical resection have been known for decades, but the mechanisms underlying treatment failures remain poorly understood. Neutrophils, first-line responders stress, may play an important role in linking inflammation to cancer progression. In response neutrophils can expel their protein-studded chromatin form local snares as neutrophil extracellular traps (NET). this study, we asked whether, a result its ability ensnare moving cells, NET formation...
Innate immunity plays a crucial role in the response to sterile inflammation such as liver ischemia/reperfusion (I/R) injury. The initiation of I/R injury results release damage‐associated molecular patterns, which trigger an innate immune and inflammatory cascade through pattern recognition receptors. Neutrophils are recruited after contribute organ damage responses. Formation neutrophil extracellular traps (NETs) has been recently found various stimuli. However, NETs during remains...
Nonalcoholic steatohepatitis (NASH) is a progressive, inflammatory form of fatty liver disease. It the most rapidly rising risk factor for development hepatocellular carcinoma (HCC), which can arise in NASH with or without cirrhosis. The signals promoting progression to HCC remain largely unknown. propensity neutrophils expel decondensed chromatin embedded proteins, known as neutrophil extracellular traps (NETs), has been shown be important chronic conditions and cancer progression. In this...
Sterile inflammatory insults are known to activate innate immunity and propagate organ damage through the recognition of extracellular damage-associated molecular pattern (DAMP) molecules. Although DAMPs such as endogenous DNA nuclear high-mobility group box 1 have been shown be critical in sterile inflammation, role histone proteins has not yet investigated. We report that histones function after ischemic injury receptor Toll-like (TLR) 9 initiate inflammation. Using an vivo model hepatic...
Hypoxia is often found in solid tumors and associated with tumor progression poor clinical outcomes. The exact mechanisms related to hypoxia-induced invasion metastasis remain unclear. We elucidated the mechanism by which nuclear-damage–associated molecular pattern molecule, high-mobility group box 1 (HMGB1), released under hypoxic stress, can induce an inflammatory response promote hepatocellular carcinoma (HCC) cells. Caspase-1 activation was occur HCC cells a process that dependent on...
Neutrophil infiltration and neutrophil extracellular traps (NET) in solid cancers are associated with poorer prognosis, but the mechanisms incompletely understood. We hypothesized that NETs enhance mitochondrial function tumor cells, providing extra energy for accelerated growth. Metastatic colorectal cancer tissue showed increased intratumoral supranormal preoperative serum MPO-DNA, a NET marker. Higher MPO-DNA correlated shorter survival. In mice, subcutaneous implants hepatic metastases...
Background Developing the ability to use tumor-directed therapies trigger potentially therapeutic immune responses against cancer antigens remains a high priority for immunotherapy. We hypothesized that histotripsy, novel non-invasive, non-thermal ablation modality uses ultrasound-generated acoustic cavitation disrupt tissues, could engender adaptive tumor antigens. Methods Immunocompetent C57BL/6 mice inoculated with flank melanoma or hepatocellular carcinoma tumors were treated thermal...
Abstract Surgical removal of malignant tumors is a mainstay in controlling most solid cancers. However, surgical insult also increases the risk tumor recurrence and metastasis. Tissue trauma activates innate immune system locally systemically, mounting an inflammatory response. Platelets neutrophils are two crucial players early response that heals tissues, but their actions may contribute to cancer cell dissemination distant Here we report stress–activated platelets enhance formation...
Cellular processes that drive sterile inflammatory injury after hepatic ischemia/reperfusion (I/R) are not completely understood. Activation of the inflammasome plays a key role in response to invading intracellular pathogens, but mounting evidence suggests it also inflammation driven by endogenous danger-associate molecular pattern molecules released ischemic injury. The nucleotide-binding domain, leucine-rich repeat containing protein 3 (NLRP3) is one such process, and mechanism which its...
Significant levels of the 1, N(2)-gamma-hydroxypropano-dG adducts alpha,beta-unsaturated aldehydes acrolein, crotonaldehyde, and 4-hydroxy-2E-nonenal (HNE) have been identified in human DNA, arising from both exogenous endogenous exposures. They yield interstrand DNA cross-links between guanines neighboring C.G G.C base pairs located 5'-CpG-3' sequences, as a result opening 1,N(2)-gamma-hydroxypropano-dG to form reactive that are positioned within minor groove duplex DNA. Using combination...
Human hepatocellular carcinoma (HCC) is driven and maintained by liver cancer stem cells (LCSCs) that display cell properties. These LCSCs are promoted the intersecting of Notch Wnt/β-Catenin signaling pathways. In this study, we demonstrate with markers CD90, CD24, CD13, CD133 possess properties self-renewal tumorigenicity in NOD/SCID mice. The increased expression these was correlated advanced disease stage, larger tumors, worse overall survival 61 HCC cases. We also found both...
The inducible nitric oxide synthase (iNOS) is associated with more aggressive solid tumors, including hepatocellular carcinoma (HCC). Notch signaling in cancer stem cells promotes progression and requires cleavage by ADAM (a disintegrin metalloprotease) proteases. We hypothesized that iNOS/NO Notch1 activation through TACE/ADAM17 liver (LCSCs), leading to a phenotype. Expression of the cell markers CD24 CD133 tumors patients HCC was greater iNOS expression worse outcomes. CD24+CD133+ LCSCs,...
High-mobility group box 1 (HMGB1) is an abundant chromatin-associated nuclear protein and released into the extracellular milieu during liver ischemia-reperfusion (I/R), signaling activation of proinflammatory cascades. Because intracellular function HMGB1 sterile inflammation I/R currently unknown, we sought to determine role in hepatocytes after I/R. When hepatocyte-specific knockout (HMGB1-HC-KO) control mice were subjected a nonlethal warm I/R, it was found that HMGB1-HC-KO had...
The dysregulation of ROS production and osteoclastogenesis is involved in the progress osteoporosis. To identify novel effective targets to treat this disease, it important explore underlying mechanisms. In our study, we firstly tested effect Nrf2 activator RTA-408, a synthetic triterpenoid under clinical investigation for many diseases, on osteoclastogenesis. We found that could inhibit osteoclast differentiation bone resorption time- dose-dependent manner. Further, RTA-408 enhanced...
Abstract Ischemia-reperfusion (I/R) injury is a process whereby an initial hypoxic insult and subsequent return of blood flow leads to the propagation innate immune responses organ injury. The necessity pattern recognition receptor, Toll-like receptor (TLR)4, for this response has been previously shown. However, TLR4 present on various cell types liver, both nonimmune cells. Therefore, we sought determine role in individual populations, specifically, parenchymal hepatocytes (HCs), myeloid...
The ability of cancer cells to survive and grow under hypoxic conditions has been known for decades, but the mechanisms remain poorly understood. Under certain conditions, undergo changes in their bioenergetic profile favor mitochondrial respiration by activating peroxisome proliferator–activated receptor gamma coactivator 1 alpha (PGC‐1α) up‐regulating biogenesis. In this study, we hypothesized that augmented biogenesis plays a critical role hypoxia. Consistent with hypothesis, both human...
Neutrophils constitute the early innate immune response to perceived infectious and sterile threats. Neutrophil extracellular traps (NETs) are a novel mechanism counter pathogenic invasion sequelae of ischemia, including cell death oxidative stress. Superoxide is radical intermediate oxygen metabolism produced by parenchymal nonparenchymal hepatic cells, hallmark stress after liver ischemia-reperfusion (I/R). While superoxide recruits neutrophils initiates inflammatory injury, it unknown...
Liver ischemia-reperfusion (I/R) injury occurs through induction of oxidative stress and release damage-associated molecular patterns (DAMPs), including cytosolic DNA released from dysfunctional mitochondria or the nucleus. Cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) synthase (cGAS) is a sensor known to trigger stimulator interferon genes (STING) downstream type 1 (IFN-I) pathways, which are pivotal innate immune system responses pathogen. However, little about role...