A5000532276- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- Collagen: Extraction and Characterization
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Ovarian cancer diagnosis and treatment
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Agriculture and Rural Development Research
- Systems Engineering Methodologies and Applications
- Cancer Research and Treatments
- Neurobiology and Insect Physiology Research
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
- Prion Diseases and Protein Misfolding
- Cancer-related Molecular Pathways
- Design Education and Practice
- Cancer-related gene regulation
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cultural Identity and Heritage
Institut Curie
2013-2024
Université Paris Sciences et Lettres
2015-2023
Centre National de la Recherche Scientifique
2012-2023
Délégation Ile-de-France Villejuif
2017
Biologie cellulaire et Cancer
2016
Centre de Gestion Scientifique
2015
Directorate-General Joint Research Centre
2015
Significance Tumor growth intrinsically generates pressure onto the surrounding tissues, which conversely compress tumor. These mechanical forces have been suggested to contribute tumor regulation. We developed a microfluidic technique produce 3D cell-based assays and interrogate interplay between mechanics in vitro. Multicellular spheroids are grown permeable elastic capsules. Capsule deformation provides direct measure of exerted pressure. By simultaneously imaging spheroid by confocal...
Diploid and stable karyotypes are associated with health fitness in animals. By contrast, whole-genome duplications-doublings of the entire complement chromosomes-are linked to genetic instability frequently found human cancers1-3. It has been established that duplications fuel chromosome through abnormal mitosis4-8; however, immediate consequences tetraploidy first interphase not known. This is a key question because single duplication events such as cytokinesis failure can promote...
Significance Intestinal epithelium damage is common but becomes recurrent in chronic intestinal disorders. Healing implies cell migration, which necessitates extensive cellular reorganization. We demonstrate that epithelial cells completely disassemble their apical actin-based microvilli upon and we identify the protein villin its actin-severing function as responsible for this physiological process. show pole effacement required acquisition of a motile phenotype efficient wound healing....
We report on a new gut chip combining the co-culture of primary epithelial and stromal cells in 3D biomimetic scaffold. Proper segregation dividing differentiated along crypt-villus axis was achieved these unique conditions.
Unscheduled whole genome duplication (WGD), also described as unscheduled or non-physiological polyploidy, can lead to genetic instability and is commonly observed in human cancers. WGD generates DNA damage due scaling defects between replication factors content. As a result, repair mechanisms are thought be critical for ensuring cell viability proliferation under these conditions. In this study, we explored the role of homologous recombination Holliday junction resolution polyploidy vivo....
The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) plays a major role in DNA damage signaling and repair is also frequently overexpressed tumor metastasis. We used isogenic cell lines expressing different levels of DNA-PKcs to investigate the metastatic development. found that participates melanoma primary metastasis development by stimulating angiogenesis, migration invasion. Comparison conditioned medium content from DNA-PKcs-proficient deficient cells reveals controls secretion...
We reported previously that the cellular prion protein (PrP(c)) is a component of desmosomes and contributes to intestinal barrier function. demonstrated also presence PrP(c) in nucleus proliferating epithelial cells. Here we sought decipher function this nuclear pool. In human cancer cells Caco-2/TC7 SW480 normal crypt-like HIEC-6 cells, interacts, cytoplasm nucleus, with γ-catenin, one its desmosomal partners, β-catenin TCF7L2, effectors canonical Wnt pathway. up-regulates transcriptional...
Cell migration has traditionally been studied in 2D substrates. However, it become increasingly evident that there is a need to study cell more appropriate 3D environments, which better resemble the dimensionality of physiological processes question. Migratory cells can substantially differ their morphology and mode depending on whether they are moving or Due technical difficulties incompatibilities with most standard protocols, structural functional analysis embedded within matrices still...
In many cell types, migration can be oriented towards a chemical stimulus. mammals, for example, embryonic cells migrate to follow developmental cues, immune toward sites of inflammation, and cancer away from the primary tumour blood vessels during metastasis. Understanding how in 3D environments response cues is thus crucial understanding directed normal disease states. To date, chemotaxis mammalian has been primarily studied using 2D models. However, it becoming increasingly clear that...
Centrosome amplification is a feature of cancer cells associated with chromosome instability and invasiveness. Enhancing subsequent cell death via centrosome unclustering multipolar divisions an aimed-for therapeutic approach. Here, we show that potentiates responses to conventional chemotherapy in addition its effect on instability. We perform single-cell live imaging epithelial ovarian lines observe increased when induced. By correlating fate mitotic behaviors, enhanced can occur...
Cell migration has traditionally been studied in 2D substrates. However, it become increasingly evident that there is a need to study cell more appropriate 3D environments, which better resemble the dimensionality of physiological processes question. Migratory cells can substantially differ their morphology and mode depending on whether they are moving or Due technical difficulties incompatibilities with most standard protocols, structural functional analysis embedded within matrices still...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation M. Verhulsel, E. Shivokhin, A. Simon, S. Descroix, C. Frétigny, L. Talini; High bandwidth noninvasive measurements of the linear viscoelasticity collagen gels. J. Rheol. 1 November 2016; 60 (6): 1269–1278. https://doi.org/10.1122/1.4965039 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends...
ABSTRACT Centrosome amplification, the presence of more than two centrosomes in a cell is common feature most human cancer lines. However, little known about centrosome numbers cancers and whether amplification or other numerical aberrations are frequently present. To address this question, we have analyzed large cohort epithelial ovarian (EOCs) from 100 patients. Using state-of-the-art microscopy, determined Centrosome-Nucleus Index (CNI) each tumor. We found that EOCs show infrequent...
Abstract There is growing evidence that the physical properties of cellular environment can impact cell migration. However, it not currently understood how active remodeling network by cells affects their migration dynamics. Here, we study collective small clusters on deformable collagen-1 networks. Combining theory and experiments, find clusters, despite displaying no apparent internal polarity, migrate persistently generate asymmetric collagen gradients during We persistent arise from...
ABSTRACT While testing for genome instability in Drosophila as reported by unscheduled upregulation of UAS-GFP cells that co-express GAL80 and GAL4, we noticed that, expected, background levels were low most developing tissues. However, GFP-positive clones frequent the larval brain. Most these originated from central brain neural stem cells. Using imaging-based approaches sequencing, show do not result chromosome loss or mutations GAL80. We have named this phenomenon ‘Illuminati’. Illuminati...
ABSTRACT Centrosome amplification is a feature of cancer cells associated with chromosome instability and invasiveness. Enhancing subsequent cell death via centrosome unclustering multipolar divisions an aimed-for therapeutic approach. Here we show that favors responses to conventional chemotherapy independently instability. We perform single-cell live imaging in epithelial ovarian lines observe increased when induced. By correlating fate mitotic behaviors, enhanced occurs identify are...