A5100460461- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Sphingolipid Metabolism and Signaling
- Cytokine Signaling Pathways and Interactions
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Erythrocyte Function and Pathophysiology
- Acute Myeloid Leukemia Research
- Psoriasis: Treatment and Pathogenesis
- Zebrafish Biomedical Research Applications
- Autophagy in Disease and Therapy
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Pancreatic function and diabetes
- Calcium signaling and nucleotide metabolism
- Advanced Biosensing Techniques and Applications
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Plant Gene Expression Analysis
- Hippo pathway signaling and YAP/TAZ
- Chemokine receptors and signaling
Xinjiang Uygur Autonomous Region Disease Prevention and Control Center
2023
People's Hospital of Xinjiang Uygur Autonomous Region
2023
Board of the Swiss Federal Institutes of Technology
2017-2021
ETH Zurich
2017-2021
Ministry of Agriculture and Rural Affairs
2020-2021
Yunnan Academy of Agricultural Sciences
2020-2021
Jimei University
2021
Education Department of Heilongjiang Province
2020
Agricultural Academy
2020
Northeast Agricultural University
2020
Hundreds of microRNAs (miRNAs) are expressed in mammalian cells, where they aid modulating gene expression by mediating mRNA transcript cleavage and/or regulation translation rate. Functional studies to date have demonstrated that several these miRNAs important during development. However, the role stem cell growth and differentiation is not well understood. We show herein microRNA (miR)-134 levels maximally elevated at day 4 after retinoic acid-induced or 2 N2B27-induced mouse embryonic...
It is critical to understand how human quiescent long-term hematopoietic stem cells (LT-HSCs) sense demand from daily and stress-mediated cues then transition into bioenergetically active progeny differentiate meet these cellular needs. However, the demand-adapted regulatory circuits of early steps hematopoiesis are largely unknown. Here we show that lysosomes, sophisticated nutrient-sensing signaling centers, regulated dichotomously by transcription factor EB (TFEB) MYC balance catabolic...
Abstract Acute myeloid leukemia (AML) is a caricature of normal hematopoiesis driven from stem cells (LSC) that share some hematopoietic cell (HSC) programs including responsiveness to inflammatory signaling. Although inflammation dysregulates mature and influences stemness lineage determination in HSCs by activating stress myelopoiesis, such roles LSCs are poorly understood. Here, we show S1PR3, receptor for the bioactive lipid sphingosine-1-phosphate, central regulator drives...
Prickles act against herbivores, pathogens or mechanical injury, while also preventing water loss. However, whether prickles have new function and the molecular genetics of prickle patterning remain poorly explored. Here, we generated a high-quality reference genome assembly for 'Basye's Thornless' (BT), prickle-free cultivar Rosa wichuraiana, to identify genetic elements related stem development. The BT harbors high level sequence diversity in itself with 'Old Blush' (R. chinensis), founder...
Abstract Homeostasis of hematopoietic stem cells (HSC) in the mammalian bone marrow cell niche is regulated by signals local microenvironment. Besides juxtacrine, endocrine and metabolic cues, paracrine autocrine are involved controlling quiescence, proliferation differentiation HSC with strong implications on expansion ex vivo as well transplantation. Towards this aim, a culture analysis polymer microcavity carrier platform was combined partial least square mechanistic model proliferation....
Allogeneic hematopoietic stem cell transplantation (HSCT) is a curative therapy, but the large number of HSCs required limits its widespread use. Host conditioning and donor composition are known to affect HSCT outcomes. However, specific role that posttransplantation signaling environment plays in HSC fate poorly understood. To mimic clinical HSCT, we injected human umbilical cord blood (UCB) cells at different doses compositions into immunodeficient NOD/SCID/IL-2Rgc-null (NSG) mice....
Protein kinase signaling regulates human hematopoietic stem/progenitor cell (HSPC) fate, yet little is known about critical pathway substrates. To address this, we have developed and applied a large‐scale, empirically optimized phosphopeptide affinity enrichment strategy with high‐throughput 2D LC‐MS/MS screening to evaluate the phosphoproteome of an isolated CD34 + HSPC population. We first used hydrophilic interaction chromatography as dimension separation separate simplify protein digest...
Abstract Synergistic interactions between cytokines underlie developmental processes fundamental to tissue and cellular engineering. However, a mechanistic understanding of the cell‐specific population‐mediated effects is often lacking. In this study, we have investigated synergistic generation erythroid cells in response erythropoietin (EPO) stem cell factor (SCF). We used quantitative approach determine if EPO SCF superpose supra‐additive fashion on proliferation rate or death rate,...
Clinical use of umbilical cord blood has typically been limited by the need to expand hematopoietic stem and progenitor cells (HSPC) ex vivo. This expansion is challenging due accumulation secreted signaling factors in culture that have a negative regulatory effect on HSPC output. Strategies for global regulation these through dilution developed, but do not accommodate dynamic nature or inherent variability cell culture. We developed mathematical model simulate impact feedback control vitro...
The ex vivo expansion of hematopoietic stem cells is significantly inhibited by secreted proteins that induce negative feedback loops. ability to effectively monitor these factors critical for their real-time regulation and control and, extension, enhancing cell expansion. Here, we describe a novel monitoring strategy the detection soluble signaling in cultures using DNA-based sensing mechanism on chip-based nanostructured microelectrode platform. We combine DNA hybridization engineering...
Herein, we describe an experimental and computational approach to perform quantitative carboxyfluorescein diacetate succinimidyl ester (CFSE) cell-division tracking in cultures of primary colony-forming unit-erythroid (CFU-E) cells, a hematopoietic progenitor cell type, which is important target for the treatment blood disorders manufacture red cells. CFSE labeling CFU-Es isolated from mouse fetal livers was performed examine effects stem factor (SCF) erythropoietin (EPO) culture. We used...
Adult bone marrow (BM) erythrocyte colony-forming units (CFU-Es) are important cellular targets for the treatment of anemia and also manufacture red blood cells (RBCs) ex vivo. We obtained quantitative biochemical measurements from single small numbers CFU-Es by isolating analyzing c-Kit(+)CD71(high)Ter119(-) adult mouse BM this allowed us to identify two mechanisms that can be manipulated increase RBC production. As expected, maximum output was when were stimulated with a combination Stem...
Abstract KLF3 is a member of the Kruppel‐like factor (KLF) family transcription factors, and plays an important role in several biological processes, including adipogenesis, erythropoiesis B‐cell development. The purposes this study are to search for polymorphisms coding region provide functional evidence abdominal fat chickens. A total 168 SNPs were detected unique chicken population, Northeast Agricultural University broiler lines divergently selected content (NEAUHLF). Of which three...