A5100414459- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Angiogenesis and VEGF in Cancer
- Cancer-related gene regulation
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Pluripotent Stem Cells Research
- Ovarian function and disorders
- Acute Myeloid Leukemia Research
- Animal Genetics and Reproduction
- Gene expression and cancer classification
- Histone Deacetylase Inhibitors Research
- Sperm and Testicular Function
- Congenital heart defects research
- Endometriosis Research and Treatment
- Prenatal Screening and Diagnostics
- Cancer Genomics and Diagnostics
- Bioinformatics and Genomic Networks
- Gene Regulatory Network Analysis
- Hematopoietic Stem Cell Transplantation
- Cancer Cells and Metastasis
- Zebrafish Biomedical Research Applications
- Immune Cell Function and Interaction
First Affiliated Hospital of Zhengzhou University
2023-2025
Zhongnan Hospital of Wuhan University
2025
Wuhan University
2025
Cornell University
2013-2025
Weill Cornell Medicine
2020-2025
Huadong Hospital
2024
Fudan University
2019-2024
Zhejiang University
2021-2024
Stanford University
2021-2024
Institute of Genetics and Developmental Biology
2022-2024
Abstract The Para rubber tree ( Hevea brasiliensis ) is an economically important tropical species that produces natural rubber, essential industrial raw material. Here we present a high-quality genome assembly of this (1.37 Gb, scaffold N50 = 1.28 Mb) covers 93.8% the (1.47 Gb) and harbours 43,792 predicted protein-coding genes. A striking expansion REF/SRPP (rubber elongation factor/small particle protein) gene family its divergence into several laticifer-specific isoforms seem crucial for...
Abstract Summary Characterizing biomarkers based on microbiome profiles has great potential for translational medicine and precision medicine. Here, we present microbiomeMarker, an R/Bioconductor package implementing commonly used normalization differential analysis (DA) methods, three supervised learning models to identify markers. microbiomeMarker also allows comparison of different methods DA confounder analysis. It uses standardized input output formats, which renders it highly scalable...
Human embryonic stem (hES) cells originate during an period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal and pluripotence. We compared the status 1536 CpG sites (from 371 genes) in 14 independently isolated hES cell lines with five other types: 24 cancer lines, four adult populations, lymphoblastoid normal human tissues, embryonal carcinoma line. found that profile clearly distinguished from all types. A subset 49 40 genes...
To examine the widely accepted dogmas that corneal grafts lack passenger leukocytes or cells capable of migrating directly to lymph nodes (LNs), we tracked migration graft-derived transgenic green fluorescent protein (GFP; Iab) into draining LNs allogeneic (Iad) recipients. GFP+ were identified in cervical several hours after transplantation, and this traffic was significantly enhanced when placed inflamed recipient beds. Draining Iab+, CD45+, CD11c+, examination ungrafted corneas revealed...
Depressed mood in pregnancy has been linked to low birth weight (LBW, < 2,500 g), a risk factor for adult-onset chronic diseases offspring. We examined maternal depressed relation and evaluated the role of DNA methylation at regulatory sequences imprinted genes this association. measured among 922 pregnant women using CES-D scale obtained data from hospital records. Using bisulfite pyrosequencing cord blood 508 infants, we differentially methylated regions (DMRs) regulating IGF2/H19,...
During red blood cell development, differentiation and cycle progression are intimately uniquely linked through interdependent mechanisms involving the erythroid transcriptional suppressor PU.1 cyclin-dependent kinase inhibitor p57KIP2.
Significance Human pluripotent stem cells (hPSCs) continue to be underappreciated as a model for studying trophoblast differentiation. In this study, we provide reproducible, two-step protocol by which hPSCs can differentiated into bipotential cytotrophoblast (CTB) stem-like and subsequently functional, terminally trophoblasts. addition, evidence that the response of hPSC-derived CTBs low oxygen is similar primary CTBs. Finally, using trisomy 21-affected hPSCs, show, first time our...
Human glioblastomas (GBM) are thought to be initiated by glioma stem-like cells (GSLCs). GSLCs also participate in tumor neovascularization transdifferentiating into vascular endothelial cells. Here, we report a critical role of the formation vasculogenic mimicry (VM), which defines channels lined supply nutrients early growing tumors and initiation. preferentially expressed growth factor receptor-2 (VEGFR-2) that upon activation VEGF, mediated chemotaxis, tubule increased expression VM...
Protein methyltransferases (PMTs) have emerged as important epigenetic regulators in myriad biological processes both normal physiology and disease conditions. However, elucidating PMT-regulated has been hampered by ambiguous knowledge about vivo activities of individual PMTs particularly because their overlapping but nonredundant functions. To address limitations conventional approaches mapping chromatin modification specific PMTs, we engineered the chromatin-modifying apparatus formulated...
In infants exposed to maternal stress in utero, phenotypic plasticity through epigenetic events may mechanistically explain increased risk of preterm birth (PTB), which confers for neurodevelopmental disorders, cardiovascular disease, and cancers adulthood. We examined associations between prenatal PTB, evaluating the role DNA methylation at imprint regulatory regions. enrolled women from clinics Durham, NC. Stress was measured 537 12 weeks gestation using Perceived Scale. differentially...
Significance A differentiated cell nucleus can be reprogrammed into the pluripotent state by maternal factors in ooplasm; that are responsible for this reprogramming process have not yet been identified. In paper, we show histone variant H3.3 is one of essential involved somatic nuclear reprogramming. Maternal H3.3, donor chromatin, required development and reactivation many key pluripotency genes transfer (SCNT) embryos. facilitates remodeling chromatin through replacement H3 with de novo...
Highlights d Genome-wide RNAi screen identifies 405 genes required for ISC maintenance Comparison of with female GSCs, and NBs finds common distinct regulators COPI/Arf79F, CycT/Cdk9, Mediator complexes
Polygonatum kingianum Collett et Hemsl. var. grandifolium D.M. Liu &amp; W.Z. Zeng (1981), which sprouts twice a year, once in spring and autumn, differs from leaves, bracts, perianth filaments. Morphological comparison molecular phylogeny indicate that it is identical to the newly-published hunanense H.H. B.Z. Wang (2021). Hence, we propose P. should be recognised as new synonym of . In addition, phylogenetic analyses confirmed sister sect. Polygonatum, rather than Verticillata.
Abstract Motivation Single-cell RNA-sequencing (scRNA-seq) is widely used to reveal cellular heterogeneity, complex disease mechanisms and cell differentiation processes. Due high sparsity gene expression patterns, scRNA-seq data present a large number of dropout events, affecting downstream tasks such as clustering pseudo-time analysis. Restoring the levels genes essential for reducing technical noise facilitating However, existing imputation methods ignore topological structure information...
Recent advances in spatial transcriptomics technologies have enabled gene expression profiles while preserving context. Accurately identifying domains is crucial for downstream analysis and it requires the effective integration of information. While increasingly computational methods been developed domain detection, most them cannot adaptively learn complex relationship between information, leading to sub-optimal performance.To overcome these challenges, we propose a novel deep learning...