A5102823885- Acute Myeloid Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Zebrafish Biomedical Research Applications
- Hematopoietic Stem Cell Transplantation
- Angiogenesis and VEGF in Cancer
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Immune cells in cancer
- CAR-T cell therapy research
- Lymphoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Acute Lymphoblastic Leukemia research
- Childhood Cancer Survivors' Quality of Life
- Phagocytosis and Immune Regulation
- Bone and Joint Diseases
- Neonatal Respiratory Health Research
- CNS Lymphoma Diagnosis and Treatment
- Axon Guidance and Neuronal Signaling
- Erythrocyte Function and Pathophysiology
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Lymphatic System and Diseases
University of Florida
2025
UF Health Cancer Center
2024
Royal Brisbane and Women's Hospital
2024
University of Florida Health
2024
Cornell University
2011-2023
Weill Cornell Medicine
2016-2023
Center for Discovery
2018-2022
Hackensack University Medical Center
2018-2022
Monash University
2019
Stem Cell Institute
2015
The bone marrow (BM) microenvironment is composed of multiple niche cells that, by producing paracrine factors, maintain and regenerate the hematopoietic stem cell (HSC) pool (Morrison Spradling, 2008Morrison S.J. Spradling A.C. Stem niches: mechanisms that promote maintenance throughout life.Cell. 2008; 132: 598-611Abstract Full Text PDF PubMed Scopus (1441) Google Scholar). We have previously demonstrated endothelial support proper regeneration system following myeloablation (Butler et...
Pluripotent stem cells (PSCs) represent an alternative hematopoietic cell (HSC) source for treating disease. The limited engraftment of human PSC–derived (hPSC-derived) multipotent progenitor (MPP) has hampered the clinical application these and suggests that MPP require additional cues definitive hematopoiesis. We hypothesized presence a vascular niche produces Notch ligands jagged-1 (JAG1) delta-like ligand-4 (DLL4) drives differentiated hes2 embryonic (hESC) Macaca nemestrina–induced PSC...
Hematopoietic stem cells (HSCs) first emerge during embryonic development within vessels such as the dorsal aorta of aorta-gonad-mesonephros (AGM) region, suggesting that signals from vascular microenvironment are critical for HSC development. Here, we demonstrated AGM-derived endothelial (ECs) engineered to constitutively express AKT (AGM AKT-ECs) can provide an in vitro niche recapitulates specification and amplification. Specifically, nonengrafting precursors, including...
Abstract Aging associated defects within stem cell-supportive niches contribute towards age-related decline in cell activity. However, mechanisms underlying niche defects, and whether restoring function can improve fitness, remain unclear. Here, we sought to determine aged blood be restored by rejuvenating their supportive the bone marrow (BM). We identify Netrin-1 as a critical regulator of BM aging. Niche-specific deletion induces premature aging phenotypes microenvironment, while...
Chronic low-grade inflammation observed in older adults, termed inflammaging, is a common feature underlying multitude of aging-associated maladies including decline hematopoietic activity. However, whether suppression inflammaging can preserve health span remains unclear, part because lack tools to measure within stem cells (HSCs). Here, we identify thrombospondin-1 (Thbs1) as an essential regulator HSCs. We describe transcriptomics-based approach for measuring and demonstrate that deletion...
Highlights•The chromatin remodeler gene Bptf shows preferential expression in primitive HSPCs•Bptf ensures maintenance and functionality of HSCs a cell-autonomous manner•Bptf is required for the activation HSC "stemness" genes including master TFs•Hematopoietic-specific loss results bone marrow failure anemiaSummarySelf-renewal differentiation adult stem cells are tightly regulated partly through configuration structure by remodelers. Using knockout mice, we here demonstrate that bromodomain...
Scaffold-guided vascular tissue engineering has been investigated as a means to generate functional and transplantable grafts that increase the efficacy of cell-based therapeutic strategies in regenerative medicine. In this study, we employed confocal microscopy three-dimensional reconstruction assess engraftment growth potential cells within an alginate scaffold with aligned pores. We fabricated honeycomb scaffolds pores, whose surface was immobilized fibronectin subsequently coated...
Abstract Clonal hematopoiesis (CH) is a common premalignant state in the blood and confers an increased risk of cancers all-cause mortality. Identification therapeutic targets CH has been hindered by lack ex vivo platform amenable for studying primary hematopoietic stem progenitor cells (HSPCs). Here, we utilize co-culture system HSPCs with bone marrow endothelial to perform CRISPR/Cas9 screens mutant HSPCs. Our data reveal that loss histone demethylase family members Kdm3b Jmjd1c...
Various factors influence the degree of leftward error (pseudoneglect) that typifies performance normal individuals in line bisection tasks. This experiment reveals eye through which stimuli are viewed also exerts a modulating on spatial attention, as indexed by significant alterations magnitude pseudoneglect. Using forced-choice tachistoscopic protocol, 24 participants (12 male; 12 female) bisected horizontally oriented lines (22.6° w × 0.39° h) presented to central vision 3 conditions:...
<p>Ex vivo characterization of BMEC/HSPC co-cultures</p>
<p>CRISPR screens in Dnmt3a- and Asxl1-mutant HSPCs Jmjd1c dependency Idh2- Tet2-mutant HSPCs</p>