A5012969489- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Erythrocyte Function and Pathophysiology
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Autism Spectrum Disorder Research
- Immune cells in cancer
- Bone and Joint Diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- T-cell and B-cell Immunology
- Behavioral and Psychological Studies
- Hematological disorders and diagnostics
- Attention Deficit Hyperactivity Disorder
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Platelet Disorders and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Prenatal Screening and Diagnostics
- Immune Response and Inflammation
- Zebrafish Biomedical Research Applications
- Folate and B Vitamins Research
- Neonatal Respiratory Health Research
- Phagocytosis and Immune Regulation
- Angiogenesis and VEGF in Cancer
University of Illinois Chicago
2019-2025
Albert Einstein College of Medicine
2014-2025
Illinois College
2021-2025
Universidade do Porto
2004-2024
California Institute for Regenerative Medicine
2014-2021
ORCID
2020
Institute for Stem Cell Biology and Regenerative Medicine
2013-2019
Centro de Estudos para a Intervencao Social
2017
Yeshiva University
2013
Institute of Cell Biology
2013
Somatic cells can be reprogrammed into induced pluripotent stem (iPS) by overexpressing combinations of factors such as Oct4, Sox2, Klf4, and c-Myc. Reprogramming is slow stochastic, suggesting the existence barriers limiting its efficiency. Here we identify senescence one barrier. Expression four reprogramming triggers up-regulating p53, p16 INK4a , p21 CIP1 . Induction DNA damage response chromatin remodeling INK4a/ARF locus are two mechanisms behind induction. Crucially, ablation...
The intermediate filament protein Nestin labels populations of stem/progenitor cells, including self-renewing mesenchymal stem cells (MSCs), a major constituent the hematopoietic cell (HSC) niche. However, intracellular location prevents its use for prospective live isolation. Hence it is important to find surface markers specific Nestin+ cells. In this study, we show that expression PDGFRα and CD51 among CD45− Ter119− CD31− mouse bone marrow (BM) stromal characterizes large fraction...
A single hematopoietic stem cell (HSC) is capable of reconstituting hematopoiesis and maintaining homeostasis by balancing self-renewal differentiation. The mechanisms HSC division balance, however, are not yet defined. Here we demonstrate, characterizing at the single-cell level a purified minimally heterogeneous murine Tie2+ population, that these top hierarchical HSCs preferentially undergo symmetric divisions. induction mitophagy, quality control process in mitochondria, plays an...
The bone marrow (BM) microenvironment is composed of multiple niche cells that, by producing paracrine factors, maintain and regenerate the hematopoietic stem cell (HSC) pool (Morrison Spradling, 2008Morrison S.J. Spradling A.C. Stem niches: mechanisms that promote maintenance throughout life.Cell. 2008; 132: 598-611Abstract Full Text PDF PubMed Scopus (1441) Google Scholar). We have previously demonstrated endothelial support proper regeneration system following myeloablation (Butler et...
How HSCs populate the fetal liver Hematopoietic stem cells (HSCs) undergo dramatic expansion in before migrating to their definitive site bone marrow. Khan et al. identify portal vessel–associated Nestin + NG2 pericytes as critical HSC niche components (see Perspective by Cabezas-Wallscheid and Trumpp). The vessel expand according fractal geometries, suggesting that cells—rather than factors expressed niche—drive proliferation. After birth, arterial vessels transform into veins, lose...
Abstract The Sialyl-Tn antigen (Neu5Acα2–6GalNAc-O-Ser/Thr) is highly expressed in several human carcinomas and associated with carcinoma aggressiveness poor prognosis. We characterized two sialyltransferases, CMP-Neu5Ac:GalNAc-R α2,6-sialyltransferase (ST6GalNAc)-I ST6GalNAc-II, that are candidate enzymes for synthases. soluble recombinant hST6GalNAc-I hST6GalNAc-II the substrate specificity of both toward a panel glycopeptides, glycoproteins, other synthetic glycoconjugates. ST6GalNAc-I...
Mitochondrial dysfunction is a primary driver of cardiac contractile failure; yet, the cross talk between mitochondrial energetics and signaling regulation remains obscure. Ponatinib, tyrosine kinase inhibitor used to treat chronic myeloid leukemia, among most cardiotoxic inhibitors causes dysfunction. Whether ponatinib-induced triggers integrated stress response (ISR) induce cardiotoxicity be determined.
Pluripotent cells develop within the inner cell mass of blastocysts, a mosaic surrounded by an extra-embryonic layer, trophectoderm. We show that set somatic lineage regulators (including Hox, Gata and Sox factors) carry bivalent chromatin enriched in H3K27me3 H3K4me2 are selectively targeted Suv39h1-mediated H3K9me3 de novo DNA methylation versus embryonic (pluripotent) lineages, as assessed both blastocyst-derived stem vivo. This stably repressed state is linked with loss gene priming for...