A5005459337- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Protein Degradation and Inhibitors
- Sperm and Testicular Function
- Cancer-related molecular mechanisms research
- Tissue Engineering and Regenerative Medicine
- RNA modifications and cancer
- Plant tissue culture and regeneration
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Bioenergy crop production and management
- Biomedical Ethics and Regulation
- Chromatin Remodeling and Cancer
- PARP inhibition in cancer therapy
- Toxin Mechanisms and Immunotoxins
- Ovarian function and disorders
- RNA Research and Splicing
- Science, Research, and Medicine
- Zebrafish Biomedical Research Applications
- Liver physiology and pathology
Cornell University
2016-2025
Weill Cornell Medicine
2020-2024
Bethune International Peace Hospital
2024
Foundation for Reproductive Medicine
2015-2023
Howard Hughes Medical Institute
2008-2014
Stem Cell Institute
2014
Institut de Médecine de la Reproduction
2014
New York Proton Center
2014
Rockefeller University
2005-2009
Chinese Academy of Sciences
2002-2006
Brdt is a testis-specific member of the distinctive BET sub-family bromodomain motif-containing proteins, motif that binds acetylated lysines and implicated in chromatin remodeling. Its expression restricted to germ line, specifically pachytene diplotene spermatocytes early spermatids. Targeted mutagenesis was used generate mice carrying mutant allele Brdt, BrdtΔBD1, which lacks only first two bromodomains uniquely characterize proteins. Homozygous BrdtΔBD1/ΔBD1mice were viable but males...
Abstract The BET subfamily of bromodomain‐containing genes is characterized by the presence two bromodomains and a unique ET domain at their carboxyl termini. Here, we show that founding member this subfamily, Brd2 , an essential gene generating mutant mouse line lacking function. Homozygous mutants are embryonic lethal, with most −/− embryos dying day 11.5. Before death, homozygous were notably smaller exhibited abnormalities in neural tube where highly expressed. Brd2‐deficient fibroblast...
Significance A differentiated cell nucleus can be reprogrammed into the pluripotent state by maternal factors in ooplasm; that are responsible for this reprogramming process have not yet been identified. In paper, we show histone variant H3.3 is one of essential involved somatic nuclear reprogramming. Maternal H3.3, donor chromatin, required development and reactivation many key pluripotency genes transfer (SCNT) embryos. facilitates remodeling chromatin through replacement H3 with de novo...
Somatic cell nuclei of giant pandas can dedifferentiate in enucleated rabbit ooplasm, and the reconstructed eggs develop to blastocysts. In order observe whether these interspecies cloned embryos implant uterus an animal other than panda, we transferred approximately 2300 panda-rabbit into 100 synchronized recipients, none became pregnant. another approach, cotransferred both cat-rabbit oviducts 21 cat recipients. Fourteen recipients exhibited estrus within 35 days; five 43–48 days after...
Background: This study explored whether the cell cycle regulator cadherin 1 (CDH1) impacts colorectal cancer and stemness via mediating ubiquitination of sirtuin 5 (SIRT5). Methods: We first constructed CDH1 overexpression plasmid small interfering RNA against SIRT5 (siSIRT5) transfected them into HCT116/HT29 cells, followed by transfection efficiency verification. The effect on Cyclin F/SIRT5/CDH1 protein levels in cells was verified Western blot. After up-regulation CDH1, changes...
Abstract Spermatogonial stem and progenitor cells (SSCs) generate adult male gametes. During in vitro expansion, these unipotent murine spontaneously convert to multipotent spermatogonial-derived (MASCs). Here we investigate this conversion process through integrative transcriptomic epigenomic analyses. We find SSCs that promoters essential maintenance differentiation of embryonic (ESCs) are enriched with histone H3-lysine4 -lysine 27 trimethylations. These bivalent modifications maintained...
Abstract Interspecies cloning may be used as an effective method to conserve highly endangered species and support the development of non‐human primate animal models for studying therapeutic nuclear–cytoplasm interaction. The use monkey model biomedical research can avoid legal, ethical, experimental limitations encountered in a clinical situation. We describe this study vitro macaca–rabbit embryos produced by fusing macaca fibroblasts with enucleated rabbit oocytes examine fate...
Tetraploid complementation is often used to produce mice from embryonic stem cells (ESCs) by injection of diploid (2n) ESCs into tetraploid (4n) blastocysts (ESC-derived mice). This method has also been adapted mouse cloning and the derivation induced pluripotent (iPS) cells. However, underlying mechanism(s) remains largely unclear. Whether this approach can give rise completely ES cell-derived an open question, not yet unambiguously proven. Here, we show that be classified two groups,...
Abstract Interspecies nuclear transfer is an invaluable tool for studying nucleus–cytoplasm interactions; and at the same time, it provides a possible alternative to clone animals whose oocytes are difficult obtain. In present study, we investigated possibility of cloning cat embryos using rabbit oocytes, compared developmental capacity; timing embryogenesis cat–rabbit cloned with that cat–cat or rabbit–rabbit embryos. When cultured in M199, rate blastocyst formation was 6.9%, which not...
Abstract Stem cells reside in specialized microenvironments created by supporting stromal that orchestrate self-renewal and lineage-specific differentiation. However, the precise identity of cellular molecular pathways support stem is not known. For example, long-term culture prototypical cells, such as adult spermatogonial progenitor (SPCs), vitro has been impeded lack an optimal cell line initiates sustains proliferation these cells. Indeed, current methods, including use mouse embryonic...
Interspecies nuclear transfer (INT) has been used as an invaluable tool for studying nucleus-cytoplasm interactions; and it may also be a method rescuing endangered species whose oocytes are difficult to obtain. In the present study, we investigated interaction of chicken genome with rabbit oocyte cytoplasm. When blastodermal cells were transferred into perivitelline space oocytes, 79.3% couplets fused 9.7% embryos developed blastocyst stage. Both M199 SOF medium culturing chicken-rabbit...
The developmental potential of hybrid embryos produced by transferring panda or cat fibroblasts into nucleated rabbit oocytes was assessed. Both the panda–rabbit and cat–rabbit were able to form blastocysts in vitro. However, rates attaining two-cell, four-cell, eight-cell, morula, blastocyst stages for hybrids significantly greater than those (P<0.05). Transferring oocytes, 31.0% rate obtained, which higher that both Whether not second polar body (PB2) extruded from one-cell (both hybrids)...
Abstract The distribution of nematode communities with depth in greenhouse soil different ages from Shenyang suburb was investigated October 2004. Thirteen families and 23 genera were observed our study. Heterocephalobus dominant genus all treatments. numbers total nematodes trophic groups declined but increased age. Bacterivores the most abundant group treatments, followed by plant parasites omnivores-predators, while fungivores least, only accounted for less than 4 % abundance. Maturity...
Abstract While Mek1/2 and Gsk3β inhibition (“2i”) supports the maintenance of murine embryonic stem cells (ESCs) in a homogenous naïve state, prolonged culture 2i results aneuploidy DNA hypomethylation that impairs developmental potential. Additionally, fails to support derivation fully potent female ESCs. Here we find mouse ESCs cultured 2i/LIF supplemented with lipid-rich albumin (AlbuMAX) undergo pluripotency transition yet maintain genomic stability full potency over long-term culture....
Summary Chromatin remodeling via incorporation of histone variants plays a key role in the regulation embryonic development. The variant H3.3 has been associated with number early events including formation paternal pronucleus upon fertilization. small amino acid differences between and its canonical counterparts (H3.1 H3.2) limited studies developmental significance deposition into chromatin due to difficulties distinguishing H3 isoforms. To this end, we used zinc‐finger nuclease (ZFN)...