A5059077365- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Circular RNAs in diseases
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Mitochondrial Function and Pathology
- Chemical Reactions and Isotopes
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Radiation Therapy and Dosimetry
- Boron Compounds in Chemistry
- Protein Degradation and Inhibitors
- Viral-associated cancers and disorders
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Nanoplatforms for cancer theranostics
- Connective tissue disorders research
- Silk-based biomaterials and applications
- Polymer Science and Applications
- HER2/EGFR in Cancer Research
- Ferroptosis and cancer prognosis
- Eosinophilic Disorders and Syndromes
Memorial Sloan Kettering Cancer Center
2018-2025
Ruijin Hospital
2024
Shanghai Jiao Tong University
2024
Kettering University
2022-2024
Nanjing Forestry University
2023
Institute of Chemical Industry of Forest Products
2023
The First Affiliated Hospital, Sun Yat-sen University
2019-2022
Sun Yat-sen University
2013-2022
Bauman Moscow State Technical University
2021
The APOBEC3 family of cytosine deaminases has been implicated in some the most prevalent mutational signatures cancer1-3. However, a causal link between endogenous enzymes and human cancer genomes not established, leaving mechanisms mutagenesis poorly understood. Here, to investigate mutagenesis, we deleted genes from cell lines that naturally generate APOBEC3-associated over time4. Analysis non-clustered clustered across whole-genome sequences 251 breast, bladder lymphoma line clones...
Chromosome-containing micronuclei are a hallmark of aggressive cancers. Micronuclei frequently undergo irreversible collapse, exposing their enclosed chromatin to the cytosol. Micronuclear rupture catalyzes chromosomal rearrangements, epigenetic abnormalities, and inflammation, yet mechanisms safeguarding micronuclear integrity poorly understood. In this study, we found that mitochondria-derived reactive oxygen species (ROS) disrupt by promoting noncanonical function charged multivesicular...
The interaction between CD155 and its high-affinity ligand TIGIT is being increasingly investigated in various solid tumors. However, the prognostic significance of lung adenocarcinoma (LUAD) remains unclear. In this study, immunohistochemistry was applied 334 LUAD cases to evaluate expression TIGIT. Western blotting conducted 5 paired primary adjacent normal tissues. Our results reveal that are overexpressed tissues aberrant overexpression closely correlated with poor clinical outcomes (P <...
Chromosomal instability is a hallmark of human cancer that associated with aggressive disease characteristics. Chromosome mis-segregations help fuel natural selection, but they risk provoking cGAS-STING immune response through the accumulation cytosolic DNA. The mechanisms how tumors benefit from chromosomal while mitigating risks, such as enhanced surveillance, are poorly understood. Here, we identify cGAS-STING-dependent upregulation nuclease TREX1 an adaptive, negative feedback mechanism...
Abstract CRISPR–Cas9-mediated homology-directed repair (HDR) can introduce desired mutations at targeted genomic sites, but achieving high efficiencies is a major hurdle in many cell types, including cells deficient DNA activity. In this study, we used genome-wide screening Fanconi anemia patient lymphoblastic lines to uncover suppressors of HDR. We found that single exonuclease, TREX1 , reduces HDR efficiency when the template single-stranded or linearized double-stranded DNA. expression...
Extrachromosomal DNA (ecDNA) are circular bodies that play critical roles in tumor progression and treatment resistance by amplifying oncogenes across a wide range of cancer types. ecDNA lack centromeres thus not constrained typical Mendelian segregation, enabling their unequal accumulation within daughter cells associated increases copy number. Despite intrinsic links to oncogenic potential, the fidelity mechanisms inheritance poorly understood. Here, we show protected against cytosolic...
SUMMARY Micronuclei are membrane-encapsulated nuclear aberrations that form following chromosome segregation errors. Micronuclear membrane collapse permits access of the pattern recognition receptor cGAS and its antagonist, TREX1 exonuclease. carboxy-terminal domain mediated endoplasmic reticulum tethering association is essential for invasion into ruptured micronuclei, however mechanisms underlying this dependency unknown. Here, we identify barrier-to-autointegration assembly factor 1 (BAF)...
This study aimed to investigate the prognostic value of potential biomarker collagen triple helix repeat containing 1 (CTHRC1) in lung adenocarcinoma (LUAD) patients.A total 210 LUAD patients diagnosed between 2003 and 2016 Department Pathology First Affiliated Hospital Sun Yat-sen University were included this study. The expression CTHRC1 vascular endothelial growth factor (VEGF), microvessel density (MVD, determined by CD34 immunostaining) evaluated immunohistochemistry tissues....
<div>Abstract<p>Chromosomal instability is a hallmark of human cancer that associated with aggressive disease characteristics. Chromosome mis-segregations help fuel natural selection, but they risk provoking cGAS-STING immune response through the accumulation cytosolic DNA. The mechanisms how tumors benefit from chromosomal while mitigating risks, such as enhanced surveillance, are poorly understood. Here, we identify cGAS-STING–dependent upregulation nuclease TREX1 an adaptive,...
Abstract Chromosomal instability, a hallmark of aggressive cancers, disrupts genome integrity through multiple hits by ongoing missegregation chromosomes. These chromosomes, inherited only one daughter cell, are subsequently encapsulated in micronuclei (MNi). Micronucleation is detrimental for replication fidelity not because it sustains chromosomal missegregation, but also MNi frequently undergo irreversible collapse during interphase exposing their enclosed chromatin to the cytosol. This...
<p>Characterization of intratumoral immune cell infiltration.</p>
<p>Frequency of micronucleation and micronuclear envelope rupture are similar across KO models.</p>
<p>Generation of KO cell lines and extracellular cGAMP measurements.</p>