A5102914219- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Renal and related cancers
- Gene Regulatory Network Analysis
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Sphingolipid Metabolism and Signaling
- Pancreatic function and diabetes
- Mechanisms of cancer metastasis
- Bacillus and Francisella bacterial research
- Advanced biosensing and bioanalysis techniques
- Listeria monocytogenes in Food Safety
- Peroxisome Proliferator-Activated Receptors
- Biomedical Ethics and Regulation
- CAR-T cell therapy research
- Arsenic contamination and mitigation
- Receptor Mechanisms and Signaling
- Cancer Genomics and Diagnostics
- Porphyrin Metabolism and Disorders
- Immune Cell Function and Interaction
- Caveolin-1 and cellular processes
- Diabetes and associated disorders
- Microbial Inactivation Methods
Kettering University
2019-2025
Memorial Sloan Kettering Cancer Center
2019-2024
Cornell University
2016-2023
Weill Cornell Medicine
2021-2023
New York Proton Center
2023
Boston University
2013
DNA methylation is essential to mammalian development, and dysregulation can cause serious pathological conditions. Key enzymes responsible for deposition removal of are known, but how they cooperate regulate the landscape remains a central question. Using knockin reporter, we performed genome-wide CRISPR-Cas9 screen in human embryonic stem cells discover regulators. The top hit was an uncharacterized gene, QSER1, which proved be key guardian bivalent promoters poised enhancers developmental...
Understanding how cells respond differently to perturbation is crucial in cell biology, but existing methods often fail accurately quantify and interpret heterogeneous single-cell responses. Here we introduce the perturbation-response score (PS), a method diverse responses at level. Applied datasets such as Perturb-seq, PS outperforms quantifying partial gene perturbations. further enables dosage analysis without needing titrate perturbations, identifies 'buffered' 'sensitive' response...
We previously demonstrated that sphingosine kinase 1 (Sphk1) expression and activity are up-regulated by exogenous palmitate (PAL) in a skeletal muscle model system diet-induced obesity mice; however, potential functions vivo relevance of this have not been addressed. Here, we aimed to determine the mechanism which PAL regulates SphK1 muscle, roles for its product, sphingosine-1-phosphate (S1P), biology context obesity. Cloning analysis mouse Sphk1 promoter revealed peroxisome...
Pluripotent stem cells have remarkable self-renewal capacity: the ability to proliferate indefinitely while maintaining pluripotent identity essential for their differentiate into almost any cell type in body. To investigate interplay between these two aspects of self-renewal, we perform four parallel genome-scale CRISPR-Cas9 loss-of-function screens interrogating fitness hPSCs and dissolution primed during early differentiation. These distinguish genes with distinct roles pluripotency...
Comprehensive enhancer discovery is challenging because most enhancers, especially those affected in complex diseases, have weak effects on gene expression. Our network modeling revealed that nonlinear enhancer-gene regulation during cell state transitions can be leveraged to improve the sensitivity of discovery. Utilizing hESC definitive endoderm differentiation as a dynamic transition system, we conducted mid-transition CRISPRi-based screen. The screen discovered comprehensive set...
Staphylococcus aureus isolated from milk and grown in was heated milk. The phenomena of death as well injury investigated the range 50 to 75 °C. D 60 value (decimal reduction time on salt-free medium) 0.87 min, D′ salt-containing 0.62 min. Cultures were injured soon heating started. This initial thermal shock increased with increasing temperature. At 50–60 °C more rapid than death. > became faster two processes coincided at 70 Z 9.46 Z′ 9.93
Understanding diverse responses of individual cells to the same perturbation is central many biological and biomedical problems. Current methods, however, do not precisely quantify strength and, more importantly, reveal new insights from heterogeneity in responses. Here we introduce perturbation-response score (PS), based on constrained quadratic optimization, at a single-cell level. Applied transcriptomes large-scale genetic datasets (e.g., Perturb-seq), PS outperforms existing methods for...
Human embryonic stem cells (hESCs) are notable for their ability to self-renew and differentiate into all tissue types in the body. NANOG is a core regulator of hESC identity, dynamic control its expression crucial maintain balance between self-renewal differentiation. Transcriptional regulation depends on enhancers, but enhancers hESCs not well characterized. Here we report two discovered from CRISPR interference screen hESCs. Deletion single copy either enhancer significantly reduced...
Abstract Pluripotent stem cells are defined by their self-renewal capacity, which is the ability of to proliferate indefinitely while maintaining pluripotent identity essential for differentiate into any somatic cell lineage. However, understanding mechanisms that control fitness versus challenging. To investigate interplay between these two aspects pluripotency, we performed four parallel genome-scale CRISPR-Cas9 loss-of-function screens interrogating in hPSC conditions, and dissolution...
Plasma fatty acids increase in obesity and diabetes; subsequent lipid overload to cells tissues mediates numerous pathological processes. This is due part aberrant synthesis of bioactive lipids. We previously demonstrated that palmitate (PAL) increased sphingosine‐1‐phosphate through regulation sphingosine kinase 1 (SphK1); however, potential functions vivo relevance this have not been addressed. Data demonstrate PAL mediated SphK1 induction PPARα. Moreover, treatment induced IL‐6 a...