A5018491438- Rheumatoid Arthritis Research and Therapies
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Hepatitis C virus research
- Multiple Sclerosis Research Studies
- Spondyloarthritis Studies and Treatments
- Cytokine Signaling Pathways and Interactions
- Autoimmune and Inflammatory Disorders Research
- Systemic Lupus Erythematosus Research
- Cervical Cancer and HPV Research
- Hepatitis B Virus Studies
- Liver Diseases and Immunity
- CAR-T cell therapy research
- Diabetes and associated disorders
- Drug-Induced Adverse Reactions
- Urticaria and Related Conditions
- Endometrial and Cervical Cancer Treatments
- Musculoskeletal synovial abnormalities and treatments
- Systemic Sclerosis and Related Diseases
- Pharmacological Effects of Natural Compounds
- Chemokine receptors and signaling
- Helicobacter pylori-related gastroenterology studies
- Autoimmune Neurological Disorders and Treatments
- Salivary Gland Disorders and Functions
Agostino Gemelli University Polyclinic
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2025
Università Cattolica del Sacro Cuore
2015-2023
Weatherford College
2022
Sapienza University of Rome
1989-2022
University of the Sacred Heart
2017
Istituto Dermopatico dell'Immacolata
2005
Fondazione Andrea Cesalpino
1988-1994
The Ag specificity and cytotoxic function of human T cell clones, generated from lymphocytes infiltrating the liver a chronic hepatitis B patient, were studied. Both class I- II-restricted clones specifically proliferated to virus envelope proteins, but not core Ag. fine cells was studied by using rAg having different composition in relation HBV-envelope proteins or synthetic peptides preS regions. antigenic determinant recognized mapped preS2 region based on response r(preS1+preS2+S)...
Abstract MicroRNA-155 (miR-155) is an important regulator of B cells in mice. have a critical role the pathogenesis rheumatoid arthritis (RA). Here we show that miR-155 highly expressed peripheral blood from RA patients compared with healthy individuals, particularly IgD - CD27 memory B-cell population ACPA + RA. MiR-155 synovial tissue containing ectopic germinal centres diffuse tissue. expression associated reciprocally lower PU.1 at level compartment. Stimulation donor CD40L, anti-IgM,...
Abstract Highly purified CD4+ T cells isolated from liver biopsies of patients with hepatitis B virus-induced CAH had a strong cytotoxic activity and were comprised substantial number (25%-40%) expressing CD56 surface marker. These absent in the peripheral blood or normal controls these suspensions did not have activity. CD4+CD56+ further characterized by studies at clonal level. A total 71 envelope antigen-specific cell clones was investigated (23 biopsies, 48 vaccinated individuals). 16...
To define the synovial characteristics of patients with rheumatoid arthritis (RA) and psoriatic (PsA) in clinical ultrasound remission achieved by combination therapy methotrexate (MTX) tumour necrosis factor (TNF) blockers.Patients RA (n=25) (disease activity score (DAS)<1.6 for at least 6 months), low disease (LDA) (n=10) (1.6<DAS<2.4 months) PsA (n=18) (DAS<1.6 Psoriasis Area Severity Index (PASI)=0 MTX+anti-TNF (adalimumab 40 mg or etanercept 50 mg) power Doppler (PDUS)-negative...
Human activated T lymphocytes expressing class II molecules are able to present only complex antigens that bind their own surface receptors, and thus can be captured, internalized, processed through the major histocompatibility processing pathway. We have used antigen-presenting cell system identify viral receptor by hepatitis B virus (HBV) enter cells, as well sequence of HB envelope antigen (HBenvAg) involved in this interaction. Results show both CD4+ CD8+ clones process HBenvAg...
Primary Sjögren’s disease is primarily driven by B-cell activation and associated with a high risk of developing non-Hodgkin’s lymphoma (NHL). Over the last few decades, microRNA-155 (miR-155) has arisen as key regulator B-cells. Nevertheless, its role in primary remains elusive. Thus, purpose this study was (i) to explore miR-155, activating factor (BAFF)-receptor (BAFF-R), Interleukin 6 receptor (IL-6R) expression labial salivary glands (LSG) patients disease, aiming identify potential...
Tenosynovial inflammation is a hallmark of rheumatoid arthritis (RA), even in the preclinical phase. However, tenosynovium retrieval and characterisation beyond current state-of-the-art. We aimed to (1) assess rate magnetic resonance imaging (MRI)-detected tenosynovitis wrists patients with clinical RA; (2) develop technique for retrieval; (3) characterise its cellular composition across disease phases, including comparison adjacent synovium. In total, 834 MRI wrist scans were analysed...
Telomere dysfunction contributes to reduced cell viability, altered differentiation, and impaired regenerative/proliferative responses. Recent advances indicate that telomerase activity confers a pro-angiogenic phenotype endothelial cells their precursors. We have investigated whether tissue regeneration following hind limb ischemia vascular growth factor 165 (VEGF165) treatment. VEGF delivery induced angiogenesis increased expression of the reverse transcriptase (TERT) in skeletal muscles...
Abstract Overweight/obesity influence disease burden and clinical outcome of Rheumatoid Arthritis (RA). The impact overweight/obesity on synovial tissue (ST) inflammation is largely unknown. Here, we investigated the histological transcriptional signature ST obtained from RA in different phases (disease onset, failure to first-line conventional DMARDs sustained ultrasound remission) finding that overweight/obese naive showed higher likelihood follicular synovitis, IHC scores for sublining...
T cell clones derived from cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) were analysed for their ability to produce interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2) and interleukin-4 (IL-4). The CSF-T compared cytokines autologous peripheral liver-infiltrating chronic active hepatitis. IL-4 production was also that by atopic patients. All the (both CD4+ CD8+) produced large amounts IFN-gamma particularly TNF-alpha. These...
Objectives: To examine synovial tissue (ST) predictors of clinical differentiation in patients with seronegative undifferentiated peripheral inflammatory arthritis (UPIA). Methods: Fourty-two IgA/IgM-Rheumatoid Factor and anti-citrullinated peptide antibodies negative UPIA, naive to Disease-Modifying Anti-Rheumatic Drugs, underwent Gray Scale (GSUS) power Doppler (PDUS) evaluation Ultrasound (US) guided ST biopsy. CD68, CD3, CD21, CD20, CD31 expression was evaluated by immunohistochemistry....
Objectives To develop an intensive training programme for ultrasound (US)-guided synovial tissue (ST) biopsy on knees and wrists in inflammatory arthritis to assess the learning curve, patient tolerability, sample quality trainees’ expectations. Methods Active or remission rheumatoid patients were enrolled. Nine trainees joined 4-month a centre experienced performing US-guided ST biopsies consisting of four sequential phases: (1) observation, (2) performance guided step-by-step phases, (3)...
Cancer frequency in muscle‐specific kinase myasthenia gravis (MuSK‐MG) has not yet been explored and the mechanisms leading to formation of MuSK IgG remain elusive. We aimed explore cancer MuSK‐MG patients assess expression cells from 2 tumors occurred this cohort. Immunohistochemistry on tumor specimens revealed primary mediastinal B cell lymphoma endometrial carcinoma. Twenty‐one males 73 females were enrolled. Fifteen cancers 13 94 (13.8%). Patients with significantly older at time onset....
Current rheumatoid arthritis (RA) treatments do not restore immune tolerance. Investigating dendritic cell (DC) populations in human synovial tissue (ST) may reveal pathways to reinstate tolerance RA. Using single-cell and spatial transcriptomics of ST biopsies, as well co-culture systems, we identified condition- niche-specific DC clusters with distinct functions. Healthy contained tolerogenic AXL
T cell clones derived from cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) were analysed for their capacity to produce interleukin 2 (IL-2), 4 (IL-4), interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha). They also compared liver-infiltrating chronic active hepatitis. All the CSF (both CD4+ CD8+) produced large amounts IFN-gamma particularly TNF-alpha, that was synthesized in a significantly larger amount than clones. Moreover, they capable secreting...
Inflammatory bowel diseases are chronic disabling conditions with a complex and multifactorial etiology, still incompletely understood. OCTN1, an organic cation transporter, could have role in modulating the inflammatory response, some genetic polymorphisms of this molecule been associated increased risk diseases. Until now, limited information exists on its potential predicting/modulating patient's response to therapies. The aim study was evaluate OCTN1 modifying gut microbiota mucosal...
In endometrial carcinoma, both L1CAM overexpression and microcystic, elongated fragmented (MELF) patterns of invasion have been related to epithelial-to-mesenchymal transition metastatic spread. We aimed assess the association between expression, MELF pattern, lymph node status in carcinoma. Consecutive cases carcinoma with pattern were immunohistochemically assessed for L1CAM. Inclusion criteria endometrioid-type, low-grade, stage T1, known status. Uni- multivariate logistic regression used...
<h3>Background</h3> Undifferentiated Peripheral Inflammatory Arthritis (UPIA) is a common diagnosis at the first clinical evaluation in rheumatological settings. However, likelihood of developing well-defined rheumatic disease UPIA patients still matter debate. <h3>Objectives</h3> To examine role ultrasound (US) and histological parameters outcome with seronegative UPIA. <h3>Methods</h3> Fourty-two IgA/IgM-Rheumatoid Factor anti-citrullinated peptide antibodies negative UPIA, naïve to any...
Summary Current rheumatoid arthritis (RA) treatments do not restore immune tolerance. Investigating dendritic cell (DC) populations in human synovial tissue (ST) may reveal pathways to re-instate tolerance RA. With single-cell and spatial-transcriptomics of biopsies, validated by micro co-culture systems, we identified condition niche-specific myeloid DC clusters with distinct differentiation trajectories functions. Healthy synovium contains a unique tolerogenic AXL pos DC2 cluster the...
Abstract Uteri from women undergoing chemoradiotherapy (CRT) may show reactive atypia which mimic serous endometrial intraepithelial carcinoma (SEIC). We aimed to assess the prevalence and morphological/immunohistochemical features of post-radiotherapy serous-like changes (PoRSEC) in undergone CRT for locally advanced cervical cancer, with a focus on differential diagnosis SEIC. Consecutive patients cancer between 2011 2018 were reviewed. Endometrial histological specimens assessed presence...