A5008850465- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Advanced Proteomics Techniques and Applications
- Colorectal Cancer Treatments and Studies
- Colorectal Cancer Surgical Treatments
- Mass Spectrometry Techniques and Applications
- Colorectal and Anal Carcinomas
- Colorectal Cancer Screening and Detection
- Tryptophan and brain disorders
- Diet and metabolism studies
- Pancreatic and Hepatic Oncology Research
- Clusterin in disease pathology
- Cancer, Lipids, and Metabolism
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Gut microbiota and health
- Peptidase Inhibition and Analysis
- Metabolomics and Mass Spectrometry Studies
- Veterinary Oncology Research
- Wnt/β-catenin signaling in development and cancer
- Telomeres, Telomerase, and Senescence
- Frailty in Older Adults
- Multiple and Secondary Primary Cancers
- Biological Research and Disease Studies
Città della Speranza Foundation
2012-2020
University of Padua
2006-2017
Houston Methodist
2015
Circulating cell‐free DNA (cfDNA) was found in increased amounts cancer patients and tumor‐associated molecular alteration can be detected patient's samples. For this reason, the cfDNA analysis is actually considered as a new concept of liquid biopsy. We evaluated presence integrity plasma by ALU‐based qPCR methylation profile OSMR SFRP1 genes promoter large cohort colorectal (CRC) ( n = 114) comparison to healthy subjects 56) with adenomatous lesions 22). Moreover, we studied prognosis...
We undertook the current study with untreated breast cancer to (1) role variations in plasma levels of cfDNA and size distribution early stage, (2) determine frequency methylation three candidate genes, RASSF1A, MAL, SFRP1, (3) whether detection changes might have specific clinical utility.Thirty-nine patients woman (median age 64 years; range, 36-90 years) who underwent surgery for primary BR 49 healthy females' subjects (control group without any lesion) were evaluated. The analyzed using...
Preoperative chemoradiotherapy is widely used to improve local control of disease, sphincter preservation and survival in patients with locally advanced rectal cancer. Patients enrolled the present study underwent preoperative chemoradiotherapy, followed by surgical excision. Response was evaluated according Mandard's Tumor Regression Grade (TRG). TRG 3, 4 5 were considered as partial or no response while 1 2 complete response. From pretherapeutic biopsies 84 carcinomas available for...
Circulating cell-free DNA (cfDNA) has been considered an interesting diagnostic/prognostic plasma biomarker in tumor-bearing subjects. In cancer patients, cfDNA can hypothetically derive from tumor necrosis/apoptosis, lysed circulating cells, and some yet unrevealed mechanisms of active release. This study aimed to preliminarily analyze dogs with canine mammary tumors (CMTs). Forty-four neoplastic, 17 non-neoplastic disease-bearing, 15 healthy were recruited. Necrosis apoptosis also assessed...
Background Currently, no reliable blood-based assay for early detection of pancreatic ductal adenocarcinoma (PDAC) is available. Cell-free DNA (cfDNA) quantitation in patients’ plasma has been recently applied monitoring several cancer types. This study evaluates the diagnostic potential cfDNA PDAC patients. Methods Plasma levels and integrity ratio were assayed using quantitative real-time PCR Alu-repeat amplicons patients with (n=50), neuroendocrine tumor (n=23), chronic pancreatitis...
Genetic screening in families with high risk to develop colorectal cancer (CRC) prevents incurable disease and permits personalized therapeutic follow-up strategies. The advancement of next-generation sequencing (NGS) technologies has revolutionized the throughput DNA sequencing.A series 16 probands for either familial adenomatous polyposis (FAP; 8 cases) or hereditary nonpolyposis (HNPCC; were investigated intragenic mutations five CRC syndromes-associated genes (APC, MUTYH, MLH1, MSH2,...
Triterpenic acid (TA) and acteoside (ACT), the major components of APPLIVER ACTEOS, respectively, have been reported to exert hepatoprotective effects, but molecular mechanisms remain elusive, particularly in NAFLD/NASH context. We assessed their effects our well-established vitro model resembling pathophysiological involved NASH. Human hepatocytes hepatic stellate cells were exposed free fatty acids (FFA) alone or combination with ACTEOS as a mono- co-culture. Steatosis, inflammation,...
<b>Background:</b> A limited number of studies aimed at investigating the possible association Y-chromosome haplogroups with microdeletions azoospermia factors (AZFs) or particular infertile phenotypes, but definitive conclusions have not been attained. The main confounding elements in these are small sample sizes and lack homogeneity geographical origin studied populations, affecting, respectively, statistical power haplogroup distribution. <b>Materials methods:</b> To assess whether some...
Tumour microsatellite instability (MSI) is useful in identifying patients with hereditary non-polyposis colorectal cancer (HNPCC) defective DNA mismatch repair (MMR} genes. A reference Bethesda panel has limitations resulting from the inclusion
// Liliana Terrin 1 , Marco Agostini 2, 3, 4 Mariagrazia Ruvoletto Andrea Martini Salvatore Pucciarelli 2 Chiara Bedin 3 Donato Nitti Patrizia Pontisso Department of Medicine, University Padua, Italy Surgery Branch, Oncology and Gastroenterology, Nano-Inspired Biomedicine Lab, Istituto di Ricerca Pediatrica - Città della Speranza, Nanomedicine, The Methodist Hospital Research Institute, Houston, TX, USA Correspondence to: Pontisso, email: patrizia@unipd.it Terrin,...
Early detection of colorectal cancer (CRC) remains a challenge. It has been highlighted that the pathological alterations within an organ and tissues might be reflected in serum or plasma proteomic/peptidic patterns. The aim study was to follow changes peptides associated progression by mass spectrometry. This included 27 adenoma, 67 CRC (n = 33 I-II stage n 34 III-IV stage), 23 liver metastasis from patients subjects disease-free as controls. For analysis, samples purification performed on...
Background Laryngeal verrucous squamous cell carcinoma (VSCC) is a highly differentiated (SCC), the diagnosis of which can meet with many pitfalls: benign hyperplastic lesions and conventional SCC are most important differential diagnoses. The microRNA miR-19a overexpressed in solid tumours regulates suppressor cytokine signalling-1 (SOCS-1) expression. Aims main endpoints were to assess SOCS-1 expression glottic VSCC, former's potential role differentiating between lesions. Methods MiR-19a...
Marioni G, Agostini M, Bedin C, Blandamura S, Stellini E, Favero Lionello Giacomelli L, Burti D’Angelo Nitti D, Staffieri A & De Filippis C (2012) Histopathology 61, 247–256 Survivin and laryngeal carcinoma prognosis: nuclear localization expression of splice variants Aims: Aberrant survivin in cancer cells has been associated with tumour progression, radiation/drug resistance shorter patient survival. The aim the present study was to investigate (LSCC) tissue – for first time at this...
Preoperative chemoradiotherapy is worldwide accepted as a standard treatment for locally advanced rectal cancer. Current of includes administration ionizing radiation 45-50.4 Gy in 25-28 fractions associated with 5-fluorouracil during therapy. Unfortunately, 40% patients have poor or absent response and novel predictive biomarkers are demanding. For the first time, we apply peptidomic methodology analysis cancer treated preoperative chemoradiotherapy. Circulating peptides (Molecular Weight...
Mass spectrometry based high throughput proteomics are used for protein analysis and clinical diagnosis. Many machine learning methods have been to construct classifiers on mass data, discrimination between cancer stages. However, the generated by such as SVM techniques typically lack biological interpretability. We present an innovative technique automated discovery of signatures optimized characterize various validate our signature algorithm one new colorectal MALDI-TOF data set, two...