A5082155174- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Ubiquitin and proteasome pathways
- Hepatocellular Carcinoma Treatment and Prognosis
- Blood Coagulation and Thrombosis Mechanisms
- Cancer, Lipids, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Diet, Metabolism, and Disease
- Monoclonal and Polyclonal Antibodies Research
- Liver Disease and Transplantation
- Systemic Lupus Erythematosus Research
- Calpain Protease Function and Regulation
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- Wnt/β-catenin signaling in development and cancer
- Liver physiology and pathology
- MicroRNA in disease regulation
- Liver Diseases and Immunity
- Cell Adhesion Molecules Research
- Protein Kinase Regulation and GTPase Signaling
University of Padua
2015-2024
Azienda Ospedaliera di Padova
1995-2023
Consorzio per la Ricerca Sanitaria
2021-2022
Center for Rheumatology
2016
Hokkaido University
2009-2013
Hospital Clínic de Barcelona
2011
Institute of Clinical and Experimental Medicine
2011
University of Bonn
2011
Centro de Investigación Biomédica en Red
2011
Universitat de Barcelona
2011
The serine protease inhibitor SerpinB3 has been described as critical mediator of liver fibrosis and it recently proposed an additional hepatokine involved in NASH development insulin resistance. Protease Activated Receptor 2 identified a novel regulator hepatic metabolism. A targeted therapeutic strategy for investigated, using 1-Piperidine Propionic Acid (1-PPA), since this compound both inhibitor. effect on inflammation genes was assessed human macrophage stellate cell lines. Transgenic...
Pathogenetic mechanisms of hepatocellular carcinoma (HCC) are still unclear and new tools for diagnostic therapeutic purposes ongoing. We have assessed whether squamous cell antigen (SCCA), a serpin overexpressed in neoplastic cells epithelial origin, is also expressed liver cancer. Squamous was evaluated by immunohistochemistry 65 HCCs different aetiology 20 normal livers. Proliferative activity using MIB-1 antibody. In 18 surgical samples, tumour nontumour tissue available SCCA cDNA...
Early detection of hepatocellular carcinoma (HCC), one the most common and deadly tumors worldwide, still is difficult due to lack adequate biomarkers that show high sensitivity specificity. The authors recently demonstrated squamous cell antigen (SCCA) variants were overexpressed remarkably in all surgically resected HCCs.For current study, assessed presence SCCA, as a free form complexed with immunoglobulins, serum from patients HCC, cirrhosis, chronic hepatitis healthy control...
Epithelial-mesenchymal transition is believed to facilitate invasion and metastasis formation of epithelial tumour cells. SERPINB3 a serine protease inhibitor, physiologically found in normal squamous epithelium but over-expressed tumours known inhibit apoptosis. We tested the hypothesis that has role by modulating epithelial-mesenchymal programme, using morphological, molecular cell biology techniques on HepG2 clones transfected with human gene. The paracrine effect this serpin was...
Hepatocellular carcinoma (HCC) is one of the most important sanitary problems for its prevalence and poor prognosis. To date, no information available on prognostic value ov-serpin SERPINB3, detected in primary liver cancer but not normal liver. The aim study was to analyse SERPINB3 expression relation with molecular signatures prognosis clinical outcome. Liver tumours 97 patients were analysed parallel TGF-β β-catenin. In a subgroup 67 adequate follow-up, correlation findings outcome also...
The only first-line treatment approved for advanced hepatocellular carcinoma (HCC) is sorafenib. Since many patients experience drug resistance, the discovery of more effective therapeutic strategies represents an unmet clinical need. MicroRNA (MiR)-122 downregulated in most HCCs, while oncogenic SerpinB3 upregulated. Here, we assessed relationship between miR-122 and their influence on cell phenotype sorafenib resistance HCC. A bioinformatics analysis identified among hypothetical targets....
SerpinB3 has been recently described as an early marker of liver carcinogenesis, but the potential mechanistic role this serpin in tumor development is still poorly understood. Overexpression Myc often correlates with more aggressive tumour forms, supporting its involvement carcinogenesis. Yes-associated protein (Yap), main effector Hippo pathway, a central regulator proliferation and it found up-regulated hepatocellular carcinomas. The study designed to investigate characterize interplay...
Non-alcoholic fatty liver disease (NAFLD) is becoming the most common chronic worldwide. In 20-30% of patients, NAFLD can progress into non-alcoholic steatohepatitis (NASH), eventually leading to fibrosis, cirrhosis and hepatocellular carcinoma development. SerpinB3 (SB3), a hypoxia-inducible factor-2α dependent cysteine protease inhibitor, up-regulated in hepatocytes during progressive proposed contribute progression. this study we investigated pro-inflammatory role SB3 by employing...
SUMMARY. Different genotypes of hepatitis C virus (HCV) have been shown to distinct geographical distribution and associate with variable clinical features. To evaluate their role in chronic Italian patients, we studied 495 consecutive cases seen nine sentinel centres homo geneously distributed over Italy. HCV genotyping was carried out using a dot‐blot hybridization assay genotype‐specific probes. Four hundred eleven patients were viraemic could be evaluated: 57% found infected HCV‐1,31%...
One of the most daunting challenges nanomedicine is finding appropriate targeting agents to deliver suitable payloads precisely cells affected by malignancies. Even more complex ability ensure that nanosystems enter those cells. Here, we use 2 nm (metal core) gold nanoparticles target human hepatocellular carcinoma (HepG2) stably transfected with SERPINB3 (SB3) protein. The were coated a 85:15 mixture thiols featuring, respectively, phosphoryl choline (to water solubility and...
Background: SerpinB3 (SB3) is a hypoxia and hypoxia-inducible factor (HIF)-2α-dependent cysteine-protease inhibitor up-regulated in hepatocellular carcinoma (HCC), released by cancer cells able to stimulate proliferation epithelial-to-mesenchymal-transition. Methods: In the study we employed transgenic knock out mice, liver cell line, human HCC specimens, mice receiving diethyl-nitrosamine (DEN) administration plus choline-deficient L-amino acid refined (CDAA) diet (DEN/CDAA protocol)....
Abstract Background In the setting of liver injury hepatic progenitor cells are activated, counterbalancing inhibited regenerative capacity mature hepatocytes. Chronic activation this compartment may give rise to a subset tumours with poor prognosis. SerpinB3, serpin over-expressed in injured and primary cancer, has been shown induce apoptosis resistance, epithelial mesenchymal transition increase TGF-beta Myc expression. Aim present study was explore presence SerpinB3 human livers mouse...
In patients with chronic hepatitis B and C virus (HBV, HCV) infection, an inverse relationship in the replicative activity of two viruses has been reported. present study genotype HCV was evaluated 34 consecutive cases found surface antigen (HBsAg) anti-HCV serum, order to identify its possible influence determining pattern HBV/HCV interaction. Nineteen were HCV-RNA positive could be genotyped: 8 infected by HCV-1 (3 HCV-1a 5 HCV-1b), 10 HCV-2, only 1 HCV-3. Among these, 3 HBV-DNA positive,...
Background Epidemiological studies indicate that a growing number of cirrhotic patients will develop hepatocellular carcinoma (HCC) in the next decade. Recent findings have demonstrated Squamous cell antigen 1 (SCCA1) and 2 (SCCA2) isoforms, now classified as serpinB3 serpinB4, are over-expressed HCC, but not normal liver. As reported, high levels circulating SCCA-IgM immunocomplexes with cirrhosis significantly associated HCC development. Aim To ascertain whether IgM-linked SCCA isoforms...
// Liliana Terrin 1 , Marco Agostini 2, 3, 4 Mariagrazia Ruvoletto Andrea Martini Salvatore Pucciarelli 2 Chiara Bedin 3 Donato Nitti Patrizia Pontisso Department of Medicine, University Padua, Italy Surgery Branch, Oncology and Gastroenterology, Nano-Inspired Biomedicine Lab, Istituto di Ricerca Pediatrica - Città della Speranza, Nanomedicine, The Methodist Hospital Research Institute, Houston, TX, USA Correspondence to: Pontisso, email: patrizia@unipd.it Terrin,...
Foot ulcerations are a disabling complication of diabetes and no treatment is currently available based on disease mechanisms. The protein serpin B3 (SB3) was identified as positive biomarker successful diabetic wound healing; therefore, its exogenous administration may promote healing. topical SB3 challenging due to nature. Physical entrapment in wet sol-gel silica can stabilize the protein's conformation permit sustained delivery. However, irreversible syneresis poor viscoelastic...
Using a solid-phase assay we have demonstrated specific competition between the preS1 sequence of hepatitis B virus and human IgA in their binding to isolated normal liver plasma membranes, suggesting molecular mimicry. Monoclonal polyclonal antibodies raised against epitopes were used detect map immunological cross-reactivity involved cell binding. These findings suggest existence common receptor or closely related receptors for attachment HBV cells.
SERPINB3 is a serine protease inhibitor with pleiotropic functions. It involved in several physiological and pathological processes, where it appears to exert antiapoptotic effects. Little known about its expression on immune system cells, the major players mechanisms of viral defense autoimmune disorders. The aim this study was characterize surface peripheral blood mononuclear cell subsets both normal subjects patients chronic infections diseases. Sixty-two were analyzed by flow cytometric...
Abnormal activation of the Wnt-β-catenin signaling cascade is involved in tumor growth and dissemination. SerpinB3 has been shown to induce β-catenin, both molecules are overexpressed tumors, particularly those with poor prognoses. The aim this study was evaluate ability modulate Wnt pathway liver cancer monocytic cells, main type inflammatory cells microenvironment. cascade, co-receptors, low-density lipoprotein receptor-related protein (LRP) members were analyzed different cell lines human...