A5056708715- Epigenetics and DNA Methylation
- COVID-19 Clinical Research Studies
- Neonatal Respiratory Health Research
- Asthma and respiratory diseases
- Effects and risks of endocrine disrupting chemicals
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Dermatology and Skin Diseases
- Long-Term Effects of COVID-19
- Pediatric health and respiratory diseases
- Cystic Fibrosis Research Advances
- Cancer-related gene regulation
- Immune responses and vaccinations
- Food Allergy and Anaphylaxis Research
- Inhalation and Respiratory Drug Delivery
- Tryptophan and brain disorders
- IL-33, ST2, and ILC Pathways
- Hormonal Regulation and Hypertension
- Thermal Regulation in Medicine
- Digestive system and related health
- Immune cells in cancer
- Vitamin D Research Studies
- Sodium Intake and Health
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020-2025
German Center for Lung Research
2019-2023
Humboldt-Universität zu Berlin
2019-2021
Freie Universität Berlin
2019-2021
Charité - Universitätsmedizin Berlin
2019-2020
Helmholtz Centre for Environmental Research
2015-2020
To investigate the immune response and mechanisms associated with severe coronavirus disease 2019 (COVID-19), we performed single-cell RNA sequencing on nasopharyngeal bronchial samples from 19 clinically well-characterized patients moderate or critical five healthy controls. We identified airway epithelial cell types states vulnerable to acute respiratory syndrome 2 (SARS-CoV-2) infection. In COVID-19, cells showed an average three-fold increase in expression of SARS-CoV-2 entry receptor...
Children have reduced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection rates and a substantially lower risk for developing disease 2019 compared with adults. However, the molecular mechanisms underlying protection in younger age groups remain unknown. Here we characterize single-cell transcriptional landscape upper airways of SARS-CoV-2-negative (n = 18) age-matched SARS-CoV-2-positive 24) children corresponding samples from adults 44), covering an range 4 weeks to 77...
In coronavirus disease 2019 (COVID-19), hypertension and cardiovascular diseases are major risk factors for critical progression. However, the underlying causes effects of main anti-hypertensive therapies—angiotensin-converting enzyme inhibitors (ACEIs) angiotensin receptor blockers (ARBs)—remain unclear. Combining clinical data (n = 144) single-cell sequencing airway samples 48) with in vitro experiments, we observed a distinct inflammatory predisposition immune cells patients that...
Pharmacological improvement of cystic fibrosis transmembrane conductance regulator (CFTR) function with elexacaftor/tezacaftor/ivacaftor (ETI) provides unprecedented improvements in lung and other clinical outcomes patients (CF). However, ETI effects on impaired mucosal homeostasis host defense at the molecular cellular levels airways CF remain unknown.
Abstract Parabens are preservatives widely used in consumer products including cosmetics and food. Whether low-dose paraben exposure may cause adverse health effects has been discussed controversially recent years. Here we investigate the effect of prenatal on childhood overweight by combining epidemiological data from a mother–child cohort with experimental approaches. Mothers reporting use paraben-containing cosmetic have elevated urinary concentrations. For butyl (BuP) positive...
Prenatal and early postnatal exposures to environmental factors are considered responsible for the increasing prevalence of allergic diseases. Although there is some evidence allergy-promoting effects in children because exposure plasticizers, such as phthalates, findings previous studies inconsistent lack mechanistic information.We investigated effect maternal phthalate on asthma development subsequent generations their underlying mechanisms, including epigenetic alterations.Phthalate...
Article24 March 2016Open Access Transparent process Environment-induced epigenetic reprogramming in genomic regulatory elements smoking mothers and their children Tobias Bauer orcid.org/0000-0002-4961-3639 Division of Theoretical Bioinformatics, German Cancer Research Center (DKFZ), Heidelberg, Germany Search for more papers by this author Saskia Trump Department Environmental Immunology, Helmholtz Centre Leipzig - UFZ, Leipzig, Naveed Ishaque Heidelberg Personalized Oncology, DKFZ-HIPO,...
Tobacco smoke is worldwide one of the main preventable lifestyle inhalative pollutants causing severe adverse health effects. Epidemiological studies revealed association tobacco smoking with epigenetic changes at single CpGs in blood. However, biological relevance often only marginal methylation remains unclear. Comparing genome-wide CpG three recently reported epidemiological datasets, two obtained on whole blood and peripheral mononuclear cells (PBMCs), it becomes evident that majority...
Exposure to endocrine-disrupting chemicals can alter normal physiology and increase susceptibility non-communicable diseases like obesity. Especially the prenatal early postnatal period is highly vulnerable adverse effects by environmental exposure, promoting developmental reprogramming epigenetic alterations. To obtain a deeper insight into role of bisphenol A (BPA) exposure in children's overweight development, we combine epidemiological data with experimental models BPA-dependent DNA...
Abstract Psychological stress during pregnancy increases the risk of childhood wheeze and asthma. However, transmitting mechanisms remain largely unknown. Since epigenetic alterations have emerged as a link between perturbations in prenatal environment an increased disease we used whole genome bisulfite sequencing (WGBS) to analyze changes DNA methylation mothers their children related psychosocial assessed its role development child. We evaluated genomic regions altered level due maternal...
Abstract Children are consistently reported to have reduced SARS-CoV-2 infection rates and a substantially lower risk for developing severe COVID-19. However, the molecular mechanisms underlying protection against COVID-19 in younger age groups remain widely unknown. Here, we systematically characterized single-cell transcriptional landscape upper airways negative age-matched positive children (n=42) corresponding samples from adults (n=44), covering an range of four weeks 77 years....
Abstract Molecular changes underlying the persistent health effects after SARS-CoV-2 infection remain poorly understood. To discern gene regulatory landscape in upper respiratory tract of COVID-19 patients, we performed enzymatic DNA methylome and single-cell RNA sequencing nasal cells patients ( n = 19, scRNA-seq 14) controls 14, 10). In addition, resampled a subset these for transcriptome analyses at 3 7) 12 months 5) post followed expression differentially regulated genes over time....
Childhood asthma is a result of complex interaction genetic and environmental components causing epigenetic immune dysregulation, airway inflammation impaired lung function. Although different microarray based EWAS studies have been conducted, the impact regulation in development still widely unknown. We therefore applied unbiased whole genome bisulfite sequencing (WGBS) to characterize global DNA-methylation profiles asthmatic children compared healthy controls.Peripheral blood samples 40...
Kynurenine formation by tryptophan-catabolic indoleamine-2,3-dioxygenase 1 (IDO1) plays a key role in tumor immune evasion and inhibition of IDO1 is efficacious preclinical models breast cancer. As the response cancer to checkpoint inhibitors may be limited, better understanding expression additional targetable immunomodulatory pathways importance. We therefore investigated regulation different subtypes. identified estrogen receptor α (ER) as negative regulator expression. Serum kynurenine...
Parabens, widely used as preservatives in cosmetics, foods, and other consumer products, are suspected of contributing to allergy susceptibility. The detection parabens the placenta or amniotic fluid raised concerns about potential health consequences for child. Recently, an increased asthma risk following prenatal exposure has been reported. Here, we investigated whether paraben can influence atopic dermatitis (AD).261 mother-child pairs German study LINA were included this analysis. Eight...
ABSTRACT The clinical course of COVID-19 is highly variable, however, underlying host factors and determinants severe disease are still unknown. Based on single-cell transcriptomes nasopharyngeal bronchial samples from clinically well-characterized patients presenting with moderate critical severities, we reveal the different types states airway epithelial cells that vulnerable for SARS-CoV-2 infection. In patients, observed a two- to threefold increase expressing entry receptor ACE2 within...
Maternal immune activation (MIA) during fetal development leads to behavioral and psychological disorders in the offspring. Concomitantly, insufficient supply of polyunsaturated fatty acids (PUFAs) is suspected contribute early neuronal maldevelopment due modulatory capabilities PUFAs. However, human data are missing considering both these aspects their impact on children's outcomes. In line, this study aimed elucidate influence gestational cytokines PUFA-containing lipids late pregnancy...
BACKGROUND: Inflammatory processes have been suggested as a culprit of vascular damage in pediatric hypertension. We aimed to investigate transcriptional changes immune modulators and determine their association with office blood pressure adolescents who were not diagnosed hypertension at the time study visit. METHODS: Office measurements samples taken from 2 German birth cohorts, GINIplus (The Infant Study on Influence Nutrition Intervention Plus Air Pollution Genetics Allergy Development;...
Hintergrund Die pharmakologische Verbesserung der CFTR-Funktion mit Elexacaftor/Tezacaftor/Ivacaftor (ETI) bewirkt deutliche Verbesserungen Lungenfunktion und anderer klinischer Parameter bei Patient:innen Mukoviszidose (zystische Fibrose, CF). Auswirkungen von ETI auf die gestörte Schleimhauthomöostase Immunabwehr molekularer zellulärer Ebene in den Atemwegen CF sind jedoch nach wie vor unbekannt. Ziel dieser Studie war es daher das Transkriptom nasalen Epithel- Immunzellen Kindern...