A5025410435- Microbial Metabolic Engineering and Bioproduction
- Distributed and Parallel Computing Systems
- Computational Physics and Python Applications
- Simulation Techniques and Applications
- Tryptophan and brain disorders
- Sirtuins and Resveratrol in Medicine
- Bioinformatics and Genomic Networks
- Calcium signaling and nucleotide metabolism
- Gene Regulatory Network Analysis
- PARP inhibition in cancer therapy
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- Metabolomics and Mass Spectrometry Studies
- Circadian rhythm and melatonin
- Light effects on plants
- Peroxisome Proliferator-Activated Receptors
- Photoreceptor and optogenetics research
- Advanced Proteomics Techniques and Applications
- PI3K/AKT/mTOR signaling in cancer
- Biochemical Acid Research Studies
- Mitochondrial Function and Pathology
- Antimicrobial Peptides and Activities
- Bipolar Disorder and Treatment
- Microtubule and mitosis dynamics
UiT The Arctic University of Norway
2014-2025
Haukeland University Hospital
2021-2025
University of Bergen
2020-2024
Friedrich Schiller University Jena
2006-2013
Ruhr University Bochum
2005-2006
Freie Universität Berlin
2005
Ras GTPase-activating protein-binding proteins 1 and 2 (G3BP1 G3BP2, respectively) are widely recognized as core components of stress granules (SGs). We report that G3BPs reside at the cytoplasmic surface lysosomes. They act in a non-redundant manner to anchor tuberous sclerosis complex (TSC) protein lysosomes suppress activation metabolic master regulator mechanistic target rapamycin (mTORC1) by amino acids insulin. Like TSC complex, G3BP1 deficiency elicits phenotypes related mTORC1...
Abstract Flagellate green algae have developed a visual system, the eyespot apparatus, which allows cell to phototax. To further understand molecular organization of apparatus and phototactic movement that is controlled by light circadian clock, detailed understanding all components needed. We procedure purify from model alga Chlamydomonas reinhardtii. Its proteomic analysis resulted in identification 202 different proteins with at least two peptides (984 total). These data provide new...
The interpretation of multi-omics datasets obtained from high-throughput approaches is important to understand disease-related physiological changes and predict biomarkers in body fluids. We present a new metabolite-centred genome-scale metabolic modelling algorithm, the Gene Expression-based Metabolite Centrality Analysis Tool (GEMCAT). GEMCAT enables integration transcriptomics or proteomics data metabolite concentrations, which can be verified by targeted metabolomics. In addition, allows...
Intrinsically disordered regions occur frequently in proteins and are characterized by a lack of well-defined three-dimensional structure. Although these do not show higher order structural organization, they known to be functionally important. Disordered rapidly evolving, largely attributed relaxed purifying selection an increased role genetic drift. It has also been suggested that positive might contribute their rapid diversification. However, for our own species, it is currently unknown...
All cells and organisms exhibit stress-coping mechanisms to ensure survival. Cytoplasmic protein-RNA assemblies termed stress granules are increasingly recognized promote cellular survival under stress. Thus, they might represent tumor vulnerabilities that currently poorly explored. The translation-inhibitory eIF2α kinases established as main drivers of granule assembly. Using a systems approach, we identify the translation enhancers PI3K MAPK/p38 pro-stress-granule-kinases. They act through...
Nicotinamide adenine dinucleotide (NAD) provides an important link between metabolism and signal transduction has emerged as central hub bioenergetics all major cellular events. NAD-dependent signaling (e.g., by sirtuins poly–adenosine diphosphate [ADP] ribose polymerases [PARPs]) consumes considerable amounts of NAD. To maintain physiological functions, NAD consumption biosynthesis need to be carefully balanced. Using extensive phylogenetic analyses, mathematical modeling metabolism,...
Substrate competition can be found in many types of biological processes, ranging from gene expression to signal transduction and metabolic pathways. Although several experimental silico studies have shown the impact substrate on these it is still often neglected, especially modelling approaches. Using toy models that exemplify different pathway scenarios, we show influence dynamics steady state concentrations a pathway. We additionally derived rate laws for reversible reactions summarise...
Tryptophan (Trp) metabolism is an important target in immuno-oncology as it represents a powerful immunosuppressive mechanism hijacked by tumors for protection against immune destruction. However, remains unclear how tumor cells can proliferate while degrading the essential amino acid Trp. Trp incorporated into proteins after attached to its tRNA tryptophanyl-tRNA synthestases. As synthestases compete with Trp-catabolizing enzymes, balance between these enzymes will determine whether used...
Tryptophan (Trp)-catabolic enzymes (TCEs) produce metabolites that activate the aryl hydrocarbon receptor (AHR) and promote tumor progression immunosuppression in glioblastoma.As therapies targeting TCEs or AHR become available, a better understanding of Trp metabolism is required.Methods: The combination LC-MS/MS with chemical isobaric labeling enabled simultaneous quantitative comparison its amino group-bearing multiple samples.We applied this method to sera cohort 43 recurrent...
ABSTRACT The unicellular flagellated green alga Chlamydomonas reinhardtii has emerged as a model organism for the study of variety cellular processes. Posttranslational control via protein phosphorylation plays key role in signal transduction, regulation gene expression, and metabolism. Thus, analysis phosphoproteome C. can significantly enhance our understanding various regulatory pathways. In this study, we have grown cultures presence an inhibitor Ser/Thr phosphatases to increase...
Tryptophan is utilized in various metabolic routes including protein synthesis, serotonin, and melatonin synthesis the kynurenine pathway. Perturbations these pathways have been associated with neurodegenerative diseases cancer. Here we present a comprehensive kinetic model of complex network human tryptophan metabolism based upon existing data for all enzymatic conversions transporters. By integrating tissue-specific expression data, modeling liver brain returned intermediate metabolite...
NAD is best known as an electron carrier and a cosubstrate of various redox reactions. However, over the past 20 years, NAD(+) has been shown to be key signaling molecule that mediates post-translational protein modifications serves precursor ADP-ribose-containing messenger molecules, which are involved in calcium mobilization. In contrast its role carrier, NAD(+)-dependent processes involve release nicotinamide (Nam) require constant replenishment cellular pools. So far, very little about...
The liver is the central hub for processing and maintaining homeostatic levels of dietary nutrients especially essential amino acids such as tryptophan (Trp). Trp required not only to sustain protein synthesis but also a precursor production NAD, neurotransmitters immunosuppressive metabolites. In light these roles its metabolic products, health well-being. regulates global supply by enzyme tryptophan-2,3-dioxygenase (TDO2), which degrades down kynurenine pathway (KP). current study, we show...
Primary human fibroblasts in tissue culture undergo a limited number of cell divisions before entering non-replicative "senescent" state. At early population doublings (PD), are proliferation-competent displaying exponential growth. During further passaging, an increasing cells become cycle arrested and finally senescent. This transition from proliferating to senescent is driven by endogenous exogenous stress factors. Here, we have developed new quantitative model for the stepwise (P) via...
Kynurenine formation by tryptophan-catabolic indoleamine-2,3-dioxygenase 1 (IDO1) plays a key role in tumor immune evasion and inhibition of IDO1 is efficacious preclinical models breast cancer. As the response cancer to checkpoint inhibitors may be limited, better understanding expression additional targetable immunomodulatory pathways importance. We therefore investigated regulation different subtypes. identified estrogen receptor α (ER) as negative regulator expression. Serum kynurenine...
Abnormal circulation in solid tumors results hypoxia, which modulates both tumor intrinsic malignant properties as well anti-tumor immune responses. Given the importance of hypoxia glioblastoma (GBM) biology and particularly shaping immunity, we analysed immunomodulatory genes are differentially regulated response to GBM cells. Gene expression analyses identified immunosuppressive enzyme tryptophan-2,3-dioxygenase (TDO2) second most downregulated gene cells cultured under hypoxic conditions....
Exploration of chronobiological systems emerges as a growing research field within bioinformatics focusing on various applications in medicine, agriculture, and material sciences. From biological perspective, the question arises whether control for regulation oscillatory signals their technical counterparts utilise similar mechanisms. If so, modelling approaches parameterisation adopted from building blocks can help to identify general components frequency circadian clocks along with gaining...