A5002609494- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Renal Diseases and Glomerulopathies
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects and Toxicity Studies
- Metabolism and Genetic Disorders
- Cell Adhesion Molecules Research
- Botulinum Toxin and Related Neurological Disorders
- Protein Structure and Dynamics
- Protein Kinase Regulation and GTPase Signaling
- Molecular Biology Techniques and Applications
- Heat Transfer Mechanisms
- Chronic Kidney Disease and Diabetes
- Infective Endocarditis Diagnosis and Management
- Tryptophan and brain disorders
- Biopolymer Synthesis and Applications
- Turbomachinery Performance and Optimization
- Anesthesia and Neurotoxicity Research
- Lipid Membrane Structure and Behavior
- Multiple Myeloma Research and Treatments
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Analytical chemistry methods development
Proteome Sciences (United Kingdom)
2003-2024
Desitin Arzneimittel (Germany)
2010-2024
Cobham (United Kingdom)
2012
Zero to Three
2009
University of Geneva
2008
Strathmann (Germany)
2005
Garmisch-Partenkirchen Medical Center
2004
Max Planck Institute of Molecular Physiology
2000-2001
Max Planck Society
1999-2001
TU Dortmund University
1999-2000
A novel MS/MS-based analysis strategy using isotopomer labels, referred to as "tandem mass tags" (TMTs), for the accurate quantification of peptides and proteins is described. The new tags are designed ensure that identical labeled with different TMTs exactly comigrate in all separations. require methods tandem spectrometry. allow from samples be identified by their relative abundance greater ease accuracy than other methods. permit simultaneous determination both identity abundances peptide...
Quantitative mass spectrometry methods offer near-comprehensive proteome coverage; however, these still suffer with regards to sample throughput. Multiplex quantitation via isobaric chemical tags (e.g., TMT and iTRAQ) provides an avenue for spectrometry-based experiments move away from simple binary comparisons toward greater parallelization. Herein, we demonstrate a straightforward method immediately expanding the throughput of reagents 6-plex 8-plex. This is based upon our ability resolve...
A new 6-plex isobaric mass tagging technology is presented, and proof of principle studies are carried out using standard protein mixtures human cerebrospinal fluid (CSF) samples. The Tandem Mass Tags (TMT) comprise a set structurally identical tags which label peptides on free amino-terminus epsilon-amino functions lysine residues. During MS/MS fragmentation, quantification information obtained through the losses reporter ions. After evaluation relative with version TMT model mixture at...
The development of the TMTpro-16plex series expanded breadth commercial isobaric tagging reagents by nearly 50% over classic TMT-11plex. In addition to described 16plex reagents, proline-based TMTpro molecule can accommodate two additional combinations heavy carbon and nitrogen isotopes. Here, we introduce final labeling TMTpro-134C TMTpro-135N, which permit simultaneous global protein profiling 18 samples with essentially no missing values. For example, six conditions three biological...
The design and synthesis of a proline-based reporter isobaric Tandem Mass Tag structure (TMTpro) is presented. An analysis made the performance new TMTpro tags in comparison with current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. provides set 16 for use resolution 6.3 mDa mass differences high spectrometers 9 reagents 1 Da spacing between ions single dalton using heavy nuclei per tag. We show similar terms peptide identification rates quantification 16-plex...
Cell-surface heparan sulfate proteoglycans have been shown to participate in lipoprotein catabolism, but the roles of specific proteoglycan classes not examined previously. Here, we studied involvement syndecan family. First, transfection CHO cells with expression vectors for several core proteins produced parallel increases cell association and degradation lipoproteins enriched lipase, a heparan-binding protein. Second, chimeric construct, FcR-Synd1, that consists ectodomain IgG Fc receptor...
N-Linked protein glycosylation is one of the most prevalent post-translational modifications and involved in essential cellular functions such as cell-cell interactions recognition well chronic diseases. In this study, we explored stable isotope labeled carbonyl-reactive tandem mass tags (glyco-TMTs) a novel approach for quantification N-linked glycans. Glyco-TMTs bearing hydrazide- aminooxy-functionalized groups were compared glycan reducing end derivatization efficiency merits. Aminooxy...
Many disease processes in the brain are reflected protein composition of cerebrospinal fluid (CSF). In addition to proteins, CSF also contains a large number endogenous peptides whose potential as biomarkers largely remains be explored. We have developed novel workflow which multiplex isobaric labeling is used for simultaneous quantification and proteins by liquid chromatography coupled with mass spectrometry. After samples, separated from ultrafiltration. The retained on filters...
Mass spectrometry-based sample multiplexing with isobaric tags permits the development of high-throughput and precise quantitative biological assays proteome-wide coverage minimal missing values. Here, we nearly doubled capability TMTpro reagent set to a 35-plex through incorporation one deuterium isotope into reporter group. Substituting frequently results in suboptimal peak coelution, which can compromise accuracy ion-based quantification. To counteract effect on quantitation, implemented...
ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionTandem Mass Tags: A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MSAndrew Thompson, Jürgen Schäfer, Karsten Kuhn, Stefan Kienle, Josef Schwarz, Günter Schmidt, Thomas Neumann, and Christian HamonCite this: Anal. Chem. 2003, 75, 18, 4942Publication Date (Web):August 8, 2003Publication History Published online8 August 2003Published inissue 1 September...
Tryptophan (Trp)-catabolic enzymes (TCEs) produce metabolites that activate the aryl hydrocarbon receptor (AHR) and promote tumor progression immunosuppression in glioblastoma.As therapies targeting TCEs or AHR become available, a better understanding of Trp metabolism is required.Methods: The combination LC-MS/MS with chemical isobaric labeling enabled simultaneous quantitative comparison its amino group-bearing multiple samples.We applied this method to sera cohort 43 recurrent...
Peripheral blood mononuclear cells (PBMCs) are an easy accessible cellular part of the organ and, along with platelets, represent only site active gene expression in blood. These undergo immunophenotypic changes various diseases and a peripheral source monitoring posttranslational modifications relevant to many diseases. Little is known about proteins we hypothesise that release from PBMCs through passive mechanisms may account for substantial plasma proteome. The use state-of-the-art...
For the study of biological signal transduction, access to correctly lipidated proteins is utmost importance. Furthermore, bioconjugates that embody correct structure protein but may additionally carry different lipid groups or labels (i.e., fluorescent tags) by which can be traced in systems, could provide invaluable reagents. We report here development techniques for synthesis a series modified Ras proteins. These number different, natural and non-natural residues, process was extended...
Isobaric labeling reagents such as Tandem Mass Tags (TMT(R)) enable the genome-wide quantification of protein expression levels under different conditions using a gel-free MS/MS-based approach. Here, we applied TMTduplex approach with two isobaric tags to study response human pathogen Neisseria meningitidis deprivation iron, condition met in body. In total, 609 proteins were identified samples three independent growth experiments, which compared cultures grown presence and absence iron....
We present a novel tandem mass tag solid-phase amino labeling (TMT-SPAL) protocol using reversible immobilization of peptides onto octadecyl-derivatized (C18) solid supports. This method can reduce the number steps required in complex protocols, saving time and potentially reducing sample loss. In our global phosphopeptide profiling workflow (SysQuant), we cut 24 h from while increasing peptide identifications (20%) side reactions. Solid-phase with TMTs does require some modification to...
To study the efficacy and safety of tolperisone – a centrally acting muscle relaxant with membrane stabilizing activity in treatment stroke‐related spasticity. This was randomized, double‐blind, placebo‐controlled, multicenter parallel groups. Treatment lasted 12 weeks started titration period variable length (dose range 300–900 mg daily). The degree spasticity determined on Ashworth Scale most severely affected joint area defined as primary target parameter. Hundred twenty patients (43...
Bartonella quintana infection can cause severe disease that includes clinical manifestations such as endocarditis, chronic bacteremia, and vasoproliferative lesions of the skin viscera. B. bacteria is transmitted by human body louse (Pediculus humanus corporis) associated with homelessness limited access to hygienic services. We report in 2 kidney transplant recipients United States from an organ donor who was experiencing homelessness. One manifested atypically, other minimally symptomatic;...
A double lipid-modified peptide which mimics the seven amino acids of C terminus N-Ras protein has been investigated by high-resolution 1H magic angle spinning NMR spectroscopy. The axially symmetric motion in bilayer decreases line widths, facilitates signal assignment and localization membrane. is located lipid/water interface lipid membrane (see schematic representation).
A novel method for the isolation of protein sequence tags to identify proteins in a complex mixture hydrophobic is described. The PST (Protein Sequence Tag) technology deals with and MS/MS based identification one N-terminal peptide from each polypeptide fragment generated by cyanogen bromide cleavage proteins. sampling takes place after sub-cellular fractionation give enrichment mitochondrial presented here combines effective sample preparation protocol involving chemical enzymatic coupled...
In this study, we present a pharmacoproteomic investigation of response to antidepressants two inbred strains. Our aim was uncover molecular mechanisms underlying antidepressant action and identify new biomarkers determine therapeutic with proven efficacy in the treatment depression but divergent action. Mice were treated pro-noradrenergic drug nortriptyline, pro-serotonergic escitalopram or saline. Quantitative proteomic analyses undertaken on hippocampal tissue from study design that used...
The progression of stem cells to proliferating progenitor and finally a quiescent differentiated state is hallmark organ development. This process proceeds through distinct steps regulated cell-cell interactions by systemically locally acting factors. We have established cell culture system which recapitulates features mammary gland development in vitro allows the comparison three characteristic differentiation stages. Cell fate decisions relating proliferation are dependent on function...