A5083329358- S100 Proteins and Annexins
- Traumatic Brain Injury and Neurovascular Disturbances
- Trypanosoma species research and implications
- Advanced Proteomics Techniques and Applications
- Acute Ischemic Stroke Management
- Mass Spectrometry Techniques and Applications
- Glaucoma and retinal disorders
- Venous Thromboembolism Diagnosis and Management
- Intracranial Aneurysms: Treatment and Complications
- Lysosomal Storage Disorders Research
- Retinal Diseases and Treatments
- Ophthalmology and Eye Disorders
- Metabolomics and Mass Spectrometry Studies
- Ocular Surface and Contact Lens
- Retinal Imaging and Analysis
- Sepsis Diagnosis and Treatment
- Parasites and Host Interactions
- Intracerebral and Subarachnoid Hemorrhage Research
- Redox biology and oxidative stress
- Wnt/β-catenin signaling in development and cancer
- Research on Leishmaniasis Studies
- Salivary Gland Disorders and Functions
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Artificial Intelligence in Healthcare
University of Geneva
2010-2019
University of Lausanne
2019
University College London Hospitals NHS Foundation Trust
2008
University College London
2008
Royal College of Physicians
2008
Mayo Clinic Hospital
2008
Alexandra Hospital
2008
Foothills Medical Centre
2008
University of Calgary
2008
DiagnoSwiss (Switzerland)
2006
Receiver operating characteristic (ROC) curves are useful tools to evaluate classifiers in biomedical and bioinformatics applications. However, conclusions often reached through inconsistent use or insufficient statistical analysis. To support researchers their ROC analysis we developed pROC, a package for R S+ that contains set of displaying, analyzing, smoothing comparing user-friendly, object-oriented flexible interface. With data previously imported into the environment, pROC builds...
A new 6-plex isobaric mass tagging technology is presented, and proof of principle studies are carried out using standard protein mixtures human cerebrospinal fluid (CSF) samples. The Tandem Mass Tags (TMT) comprise a set structurally identical tags which label peptides on free amino-terminus epsilon-amino functions lysine residues. During MS/MS fragmentation, quantification information obtained through the losses reporter ions. After evaluation relative with version TMT model mixture at...
Purpose Multiple sclerosis is the first cause of progressive neurological disability among young adults living in W estern countries. Its diagnosis mostly based on clinical evaluation, neuroimaging, and some cases cerebrospinal fluid ( CSF ) analysis, but no definitive diagnostic test exists. We proposed here that exploration tears from multiple patients could lead to discovery new biomarkers. Experimental design Thirty (20% men) recruited Geneva University Hospitals were included our study...
Parkinson's disease (PD) pathology spreads throughout the brain following a region-specific process predominantly affecting substantia nigra (SN) pars compacta. SN exhibits progressive loss of dopaminergic neurons responsible for major cardinal motor symptoms, along with occurrence Lewy bodies in surviving neurons. To gain new insights into underlying pathogenic mechanisms PD, we studied postmortem nigral tissues dissected from pathologically confirmed PD cases (n = 5) and neurologically...
In vivo human brain extracellular fluids (ECF) of acute stroke patients were investigated to assess the changes in protein levels associated with ischemic damages. Microdialysates (MDs) from infarct core (IC), penumbra (P), and unaffected contralateral (CT) regions suffering an (n = 6) compared using a shotgun proteomic approach based on isobaric tagging mass spectrometry. Quantitative analysis showed 53 proteins increased amounts IC or P respect CT samples. Glutathione S-transferase...
The majority of patients with mild traumatic brain injury (mTBI) will have normal Glasgow coma scale (GCS) 15. Furthermore, only 5%-8% them be CT-positive for an mTBI. Having a useful biomarker would help clinicians evaluate patient's risk developing intracranial lesions. S100B protein is currently the most studied and promising this purpose. Heart fatty-acid binding (H-FABP) has been highlighted in models investigated as stroke severe TBI, example. Here, we performances H-FABP...
Ocular fluids from patients with retinal detachment show iron accumulation, and transferrin can be used as a therapeutic adjuvant.
Background Human African trypanosomiasis (HAT), also known as sleeping sickness, is a parasitic tropical disease. It progresses from the first, haemolymphatic stage to neurological second due invasion of parasites into central nervous system (CNS). As treatment depends on disease, there critical need for tools that efficiently discriminate two stages HAT. We hypothesized markers brain damage discovered by proteomic strategies and inflammation-related proteins could individually or in...
Accurate stage determination is crucial in the choice of treatment for patients suffering from sleeping sickness, also known as human African trypanosomiasis (HAT). Current staging methods, based on counting white blood cells (WBC) and detection parasites cerebrospinal fluid (CSF) have limited accuracy. We hypothesized that immune mediators reliable T. b. gambiense HAT could be used to stratify rhodesiense patients, less common form HAT.A population comprising 85 14 1 (S1) 71 2 (S2) enrolled...
Inflammation is known to worsen cerebral damage at the acute phase of stroke. In this setting, cell adhesion molecules (CAMs) play a crucial role mediating migration immune cells into infarcted area. However, their value in long-term outcome prediction for patients with cerebrovascular diseases (CVD) less described.Levels four CAMs (E-selectin, P-selectin glycoprotein ligand-1, intercellular molecule-1, and vascular molecule-1 (VCAM-1)) six other biomarkers (C-reactive protein (CRP),...
The aim was to investigate the levels of cytokines and soluble IL-6R in tears patients with thyroid-associated orbitopathy (TAO) disease. Schirmer's test adopted collect from TAO (N = 20, 17 women, mean age (±SD): 46.0 years (±13.4)) healthy subjects 18, 10 45.4 (±18.7)). Lacrimal (sIL-6R) were measured using a 10-plex panel (Meso Scale Discovery Company) Invitrogen Human sIL-6R Elisa kit, respectively. Tear IL-10, IL-12p70, IL-13, IL-6 TNF-α appeared significantly higher than subjects....
In the past few years, mass spectrometry (MS) has emerged as an efficient tool for multiplexed peptide and protein concentration determination by isotope dilution. Despite growing use of isobaric tagging to perform relative quantitation discovery potential biomarkers in biological fluids, no real application so far been presented their absolute quantitation. Isobaric tandem tags (TMTs) were used herein selection tryptic peptides derived from brain damage related proteins cerebrospinal fluid...
Human African trypanosomiasis, or sleeping sickness, is a parasitic disease endemic in sub-Saharan Africa, transmitted to humans through the bite of tsetse fly. The first hemolymphatic stage associated with presence parasites bloodstream, lymphatic system, and body tissues. If patients are left untreated, cross blood-brain barrier invade cerebrospinal fluid brain parenchyma, giving rise second meningoencephalitic stage. Stage determination crucial step guiding choice treatment, as drugs used...
Background Sleeping sickness, or human African trypanosomiasis (HAT), is a protozoan disease that affects rural communities in sub-Saharan Africa. Determination of the stage, essential for correct treatment, represents key issue management patients. In present study we evaluated potential CXCL10, CXCL13, ICAM-1, VCAM-1, MMP-9, B2MG, neopterin and IgM to complement current methods staging Trypanosoma brucei gambiense Methods Findings Five hundred twelve T. b. HAT patients originated from...
Abstract Objective: Wide interindividual variability exists in response to tissue plasminogen activator (t‐PA) treatment the acute phase of ischemic stroke. We aimed find genetic variations associated with hemorrhagic transformation (HT) and mortality rates after t‐PA. then generated a clinical–genetic model for predicting t‐PA response. Methods: Our prospective study used SNPlex genotype 140 single nucleotide polymorphisms (SNPs) from 97 candidate genes 3 patient cohorts. The cohorts...
In order to increase their predictive power, medical biomarkers can be combined into panels. However, the lack of ready-to-use tools generating interpretable results and implementing rigorous validation standards hampers more widespread application panels translation clinical practice. The computational toolbox we present here – PanelomiX uses iterative combination thresholds (ICBT) method. This method combines scores by selecting that provide optimal classification performance. To speed up...
Purpose: To investigate the molecular composition of subretinal fluid (SRF) in central serous chorioretinopathy (CSCR) and rhegmatogenous retinal detachment (RRD) using proteomics metabolomics. Methods: SRF was obtained from one patient with severe nonresolving bullous CSCR requiring surgical fibrin removal, two patients long-standing RRD. Proteins were trypsin-digested, labeled Tandem-Mass-Tag fractionated according to their isoelectric point for identification quantification by tandem mass...