A5103108348- Photoreceptor and optogenetics research
- Mass Spectrometry Techniques and Applications
- Retinal Development and Disorders
- RNA and protein synthesis mechanisms
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Trace Elements in Health
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Protein Structure and Dynamics
- Neurological disorders and treatments
- Protein Hydrolysis and Bioactive Peptides
- Parkinson's Disease Mechanisms and Treatments
- Neural dynamics and brain function
- Viral Infections and Immunology Research
- Plant and Biological Electrophysiology Studies
- Ion channel regulation and function
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Neuropeptides and Animal Physiology
- Botulinum Toxin and Related Neurological Disorders
- Bacterial Genetics and Biotechnology
- Analytical Chemistry and Chromatography
- Lipid Membrane Structure and Behavior
University of Zurich
2017-2024
University Hospital of Zurich
2021-2023
University of Bern
2020-2022
GlaxoSmithKline (United States)
2020
Agroscope
2017-2018
Social Neuroscience Lab
2015
University of Würzburg
2013
Institut des Maladies Métaboliques et Cardiovasculaires
2008
Proteome Sciences (United Kingdom)
2003
Royal Holloway University of London
2000
In the frame of COST action INFOGEST, a static in vitro digestion protocol has been elaborated aiming at improvement data comparability by harmonizing experimental conditions. The success harmonization was confirmed with inter-laboratory trials using skim milk powder as standardized model food. Moreover, physiological relevance gastric and intestinal endpoints demonstrated pig vivo trial, same samples collected from different sections digestive tract, well human study jejunal effluents....
Recently, a static in vitro digestion (IVD) protocol was published by Minekus and coworkers (Minekus et al., 2014) within the COST INFOGEST network. The protocol, concentrating on physiological enzyme activities had main goal to improve comparability of experimental data between labs. validated several inter-laboratory studies using skim milk powder (SMP) indeed demonstrated improved harmonization compared with previous experiments individual IVD protocols (Egger 2016). Although salt...
By the use of different Corynebacterium glutamicum strains more than 1.4 million tons amino acids, mainly L-glutamate and L-lysine, are produced per year. A project was started recently to elucidate complete DNA sequence this bacterium. In communication we describe an approach analyze C. proteome, based on genetic information, by a combination two-dimensional (2-D) gel electrophoresis protein identification via microsequencing or mass spectrometry. We used these techniques resolve proteins...
The serotonin (5-HT) and neuropeptide S (NPS) systems are discussed as important genetic modulators of fear sustained anxiety contributing to the etiology disorders. Sustained is a crucial characteristic most disorders which likely develops through contextual conditioning. This study investigated if how alterations 5-HT NPS well their interaction modulate conditioning; specifically, function polymorphic variants in genes coding for transporter (5HTT) receptor (NPSR1) were studied. A large...
The SARS-CoV-2 Delta variant, corresponding to the Pangolin lineage B.1.617.2, was first detected in India July 2020 and rapidly became dominant worldwide. ARTIC v3 protocol for whole-genome sequencing, which relies on a large number of PCR primers, among available early pandemic, but may be prone coverage dropouts that result incomplete genome sequences. A new set primers (v4) designed circumvent this issue June 2021. In study, we investigated whether sequencing community adopted sets...
Isobaric labeling reagents such as Tandem Mass Tags (TMT(R)) enable the genome-wide quantification of protein expression levels under different conditions using a gel-free MS/MS-based approach. Here, we applied TMTduplex approach with two isobaric tags to study response human pathogen Neisseria meningitidis deprivation iron, condition met in body. In total, 609 proteins were identified samples three independent growth experiments, which compared cultures grown presence and absence iron....
In this study, we present an NMR structure of the metallothionein (MT) from snail Littorina littorea (LlMT) in complex with Cd2+ . LlMT is capable binding 9 Zn2+ or ions. Sequence alignments other MTs revealed that protein likely composed three domains. The study divided into individual domains, each which folds a single well-defined three-metal cluster. central α2 and C-terminal β domains are positioned unique relative orientation. Two variants longer shorter linkers were investigated,...
A searchable library of MS/MS spectra obtained using a quadrupole ion trap mass spectrometer and electrospray or atmospheric pressure chemical ionization is presented. The application wideband excitation (activation) normalized collision energy leads to highly reproducible which are searched the NIST algorithm. Flow injection LC/MS/MS applications this powerful technique in biomedical (diastereoisomeric steroids, morphine glucuronides, isovalerylcarnitine) environmental (pirimicarb...
Abstract The patch clamp technique has been applied to isolated vacuoles from green suspension cells of Chenopodium rubrum record electrical parameters the tonoplast. In a symmetrical K + solution 46 mᴍ, membrane displays near zero voltage, whereas 2 mᴍ ATP will hyper-polarize it 15 or 20 mV (vacuole positive). conductance amounts about one S · m -2 . Fluctuations current are explained by an unknown channel species having opening times 5 - 10 ms. Together with previous work on tonoplast...
Sample preparation for NMR studies of G protein-coupled receptors faces special requirements: Proteins need to be stable prolonged measurements at elevated temperatures, they should ideally uniformly labeled with the isotopes 13C, 15N, and all carbon-bound protons replaced by deuterons. In addition, certain experiments require protonated methyl groups in presence a perdeuterated background. All these requirements are most easily satisfied when using Escherichia coli as expression host. Here...
The tonoplast of isolated vacuoles from photoautotrophic suspension cells Chenopodium rubrum L. was studied by means the patch-clamp technique. In a symmetrical K(+) concentration 46 mM, similar to in vivo conditions, displayed membrane potential near zero and linear current-voltage relationship with mean slope 1.0 S/m(2). ATP at 2 mM hyperpolarized (vacuole positive) 15-20 mV and, parallel experiment, acidified vacuole (outside pH 7.0) 5.0, as monitored accumulation acridine orange....
Accumulating evidence from mouse models points to the G protein‐coupled receptor RGS2 (regulator of G‐protein signaling 2) as a promising candidate gene for anxiety in humans. Recently, polymorphisms were found be associated with various disorders, e.g., rs4606 panic disorder (PD), but other findings have been negative or inconsistent concerning respective risk allele. To further examine role pathogenesis PD, we genotyped and five additional tag single nucleotide (SNPs; rs16834831,...
We performed isoform-specific MUP quantification on MS1 and MS2 level in response to increased social interaction of male wild house mice by seminatural housing.
Abstract The tiny contribution of cadmium (Cd) to the composition earth’s crust contrasts with its high biological significance, owing mainly competition Cd essential zinc (Zn) for suitable metal binding sites in proteins. In this context it was speculated that several animal lineages, protein family metallothioneins (MTs) has evolved specifically detoxify Cd. Although multi-functionality and heterometallic MTs most species does not support such an assumption, there are some exceptions role,...
Enteroviruses are small RNA viruses that affect millions of people each year by causing an important burden disease with a broad spectrum symptoms. In routine diagnostic laboratories, enteroviruses identified PCR-based methods, often combined partial sequencing for genotyping. this proof-of-principle study, we assessed direct (DRS) using nanopore technology fast whole-genome directly from clinical samples. The approach was complemented the corresponding viral cDNA via Illumina MiSeq...
Leucine and Isoleucine are two amino acids that differ only by the positioning of one methyl group. This small difference can have important consequences in α-helices, as β-branching Ile results helix destabilization. We set out to investigate whether there general trends for occurrences Leu residues structures sequences class A GPCRs (G protein-coupled receptors). integral membrane proteins which α-helices span plasma seven times play a crucial role signal transmission. found side chains...
A novel method for the isolation of protein sequence tags to identify proteins in a complex mixture hydrophobic is described. The PST (Protein Sequence Tag) technology deals with and MS/MS based identification one N-terminal peptide from each polypeptide fragment generated by cyanogen bromide cleavage proteins. sampling takes place after sub-cellular fractionation give enrichment mitochondrial presented here combines effective sample preparation protocol involving chemical enzymatic coupled...
Biochemical evidence suggests that the asialoglycoprotein receptor (ASGP-R) can be used as an alternative for a temperature-sensitive Sendai virus (SV) mutant. We now have investigated this possible route of infection SV wild-type (SV-wt) strain Fushimi by using pair cell lines which differ only with regard to ASGP-R expression. Infection studies after enzymatic destruction conventional sialic acid-containing receptors (SA-R) revealed ASGP-R-expressing cells could infected SV-wt. This entry...
Abstract Resonance assignments are challenging for membrane proteins due to the size of lipid/detergent-protein complex and presence line-broadening from conformational exchange. As a consequence, many correlations missing in triple-resonance NMR experiments typically used assignments. Herein, we present an approach which these solution-state supplemented by data 13 C unlabeling, single-amino acid type labeling, 4D NOESY proximity moieties lipids or water combination with structure protein....
Metallothioneins (MTs) are small proteins that coordinate d-block metal ions in sulfur-metal clusters to control ion concentrations within the cell. Here we study cluster formation MT of periwinkle Littorina littorea (LlMT) by nuclear magnetic resonance (NMR). We demonstrate three Cd