A5073307552- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- Congenital Diaphragmatic Hernia Studies
- Machine Learning in Bioinformatics
- Animal Virus Infections Studies
- Neonatal Respiratory Health Research
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Immune responses and vaccinations
- Asthma and respiratory diseases
University of Bern
2017-2024
Federal Department of Home Affairs
2020
The SARS-CoV-2 Delta variant, corresponding to the Pangolin lineage B.1.617.2, was first detected in India July 2020 and rapidly became dominant worldwide. ARTIC v3 protocol for whole-genome sequencing, which relies on a large number of PCR primers, among available early pandemic, but may be prone coverage dropouts that result incomplete genome sequences. A new set primers (v4) designed circumvent this issue June 2021. In study, we investigated whether sequencing community adopted sets...
Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage characterization in diagnostic laboratories worldwide. The rapid, near-full-length of viral genome is commonly enabled by high-throughput PCR amplicons derived from cDNA molecules. Here, we present a new approach called NASCarD (Nanopore Adaptive Sampling with Carrier DNA), which allows low amount nucleic acids to be sequenced while selectively enriching sequences interest, hence limiting production...
The human respiratory syncytial virus (RSV) is a major cause of severe lower tract infections in infants, possibly due to the properties immature neonatal pulmonary immune system. Using newborn lamb, classical model lung development and translational RSV infection, we aimed explore role cell-mediated immunity disease during early life. Remarkably, healthy conditions, developing T cell compartment showed differences that seen mature adult lung. most striking observation being high baseline...
Abstract Background Testing and contact tracing (CT) can interrupt transmission chains of SARS-CoV-2. Whole-genome sequencing (WGS) potentially strengthen these investigations provide insights on transmission. Methods We included all laboratory-confirmed COVID-19 cases diagnosed between 4 June 26 July 2021, in a Swiss canton. defined CT clusters based epidemiological links reported the data genomic as sequences with no single-nucleotide polymorphism (SNP) differences any 2 pairs being...
ABSTRACT Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage characterization in diagnostic laboratories worldwide. The rapid, near-full-length of viral genome is commonly enabled by high-throughput PCR amplicons derived from cDNA molecules. Here, we present a new approach, called NASCarD (Nanopore adaptive sampling with carrier DNA), which allows low amount nucleic acids to be sequenced while selectively enriching sequences interest, hence limiting production...
ABSTRACT The human respiratory syncytial virus (RSV) is a major cause of severe lower tract infections in infants, possibly due to the specific features immature neonatal pulmonary immune system. Using newborn lamb, classical model lung development and state-of-the-art RSV infection, we aimed explore role cell-mediated immunity disease during early life. Remarkably, healthy conditions, developing T cell compartment showed differences that seen mature adult lung. most striking observation...
Abstract We are reporting on the observation of a large, under-sequenced region S gene SARS-CoV2 Delta variant genomes, identified in sequences originating from various sequencing centres worldwide (e.g. USA, India, England, Switzerland, France, Germany). This poorly sequenced was early phases spread and phenomenon is still ongoing. As many commonly-used protocols rely amplicon-based procedures, we investigated likely origin issue. established its biological as resulting mutations viral...