A5064611708- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Reproductive Physiology in Livestock
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- Poxvirus research and outbreaks
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Agriculture and Farm Safety
- Rabies epidemiology and control
- Veterinary Medicine and Surgery
- Influenza Virus Research Studies
- Microbial infections and disease research
- Veterinary Equine Medical Research
- Insect and Pesticide Research
- Reproductive System and Pregnancy
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Vector-borne infectious diseases
- Plant Virus Research Studies
- Estrogen and related hormone effects
- Hormonal and reproductive studies
Friedrich-Loeffler-Institut
2016-2025
Leibniz Centre for Agricultural Landscape Research
2024
University of Giessen
2010-2022
Clemenshospital Münster
2022
Sylhet Agricultural University
2021
Animal Health Institute
2021
Bangladesh Agricultural University
2021
University of Bern
2020
University of Lübeck
2018
University Medical Center
2018
Abstract In 2011, an unidentified disease in cattle was reported Germany and the Netherlands. Clinical signs included fever, decreased milk production, diarrhea. Metagenomic analysis identified a novel orthobunyavirus, which subsequently isolated from blood of affected animals. Surveillance initiated to test malformed newborn animals region.
Between 2011 and 2013, three breeders of variegated squirrels (Sciurus variegatoides) had encephalitis with similar clinical signs died 2 to 4 months after onset the symptoms. With use a metagenomic approach that incorporated next-generation sequencing real-time reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR), presence previously unknown bornavirus was detected in contact squirrel brain samples from patients. Phylogenetic analyses showed this virus, tentatively named 1...
Foot-and-mouth disease (FMD) is a trans-boundary viral of livestock, which causes huge economic losses and constitutes serious infectious threat for livestock farming worldwide. Early diagnosis FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay detection virus (FMDV). The FMDV RT-RPA design targeted 3D gene 260 nt molecular RNA standard was used...
The genus Pestivirus within the family Flaviviridae currently consists of four different main species: Classical swine fever virus, Bovine viral diarrhea virus types 1 and 2 Border disease virus. A fifth tentative species is represented by an isolate from a giraffe. In this study, completely new pestivirus, isolated batch fetal calf serum that was collected in Brazil, described. It proposed D32/00_'HoBi' may constitute novel sixth pestivirus species, because it genetically, as well...
Hepatitis C virus (HCV) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. Research complicated by a lack accessible small animal models. The systematic investigation viruses mammals could guide efforts to establish such models, while providing insight into viral evolutionary biology. We have assembled so-far largest collection small-mammal samples from around world, qualified be screened for bloodborne viruses, including sera organs 4,770 rodents (41...
Newcastle disease virus (NDV)-expressing avian influenza (AIV) hemagglutinin (HA) of subtype H5 was constructed by reverse genetics. A cloned full-length copy the genome lentogenic NDV strain Clone 30 used for insertion ORF encoding HA highly pathogenic AIV isolate A/chicken/Italy/8/98 (H5N2) in intergenic region between fusion and hemagglutinin-neuraminidase (HN) genes. Remarkably, two species transcripts were detected cells infected with resultant NDVH5. In a second recombinant (NDVH5m),...
This study aimed to identify the causative agent of mass mortality in wild and captive birds southwest Germany gather insights into phylogenetic relationship spatial distribution pathogen. Since June 2011, 223 dead were collected tested for presence viral pathogens. Usutu virus (USUV) RNA was detected by real-time RT-PCR 86 representing 6 species. The isolated cell culture from heart 18 Blackbirds (Turdus merula). USUV-specific antigen demonstrated immunohistochemistry brain, heart, liver,...
ABSTRACT A synopsis is presented of hormonal profiles (peripheral plasma values LH, FSH, progesterone; urinary oestrogens) during the oestrous cycle cattle. After ovulation (day one) progesterone levels begin to increase from about day 4, reach a plateau around 10, and decrease basal concentrations starting on days 17 18. Corresponding with decrease, oestrogens show main reaching its maximum one before ovulation. LH are fairly constant until 5, small but significant peaks can be observed...
Abstract During 2000–2013, 4 genotypes of bluetongue virus (BTV) were detected in Corsica, France. At the end 2013, a compulsory BTV-1 vaccination campaign was initiated among domestic ruminants; biological samples from goats tested as part corresponding monitoring program. A BTV strain with nucleotide sequences suggestive novel serotype detected.
In mid September 2008, clinical signs of bluetongue (particularly coronitis) were observed in cows on three different farms eastern Netherlands (Luttenberg, Heeten, and Barchem), two which had been vaccinated with an inactivated BTV-8 vaccine (during May-June 2008). Bluetongue virus (BTV) infection was also detected a fourth farm (Oldenzaal) the same area while testing for export. BTV RNA subsequently identified by real time RT-PCR targeting genome-segment (Seg-) 10, blood samples from each...
Abstract Rapid and sensitive diagnostic approaches are of the utmost importance for detection humans animals infected by specific influenza virus subtype(s). Cascade-like diagnostics starting with use pan-influenza assays subsequent subtyping devices normally used. Here, we demonstrated a novel low density array combining 32 TaqMan ® real-time RT-PCR systems in parallel haemagglutinin (HA) neuraminidase (NA) subtypes avian porcine hosts. The sensitivity newly developed system was compared...
During the compulsory vaccination programme against bluetongue virus serotype 1 (BTV-1) in Corsica (France) 2014, a BTV strain belonging to previously uncharacterized (BTV-27) was isolated from asymptomatic goats. The present study describes detection and molecular characterization of two additional distinct BTV-27 variants found goats 2014 2015. full coding genome these novel show high homology (90-93 % nucleotide/93-95 amino acid) with originally described isolate Corsican 2014. These...
Cowpox virus (CPXV) was considered as uniform species within the genus Orthopoxvirus (OPV). Previous phylogenetic analysis indicated that CPXV is polyphyletic and isolates may cluster into different clades with two of these showing genetic similarities to either variola (VARV) or vaccinia viruses (VACV). Further analyses were initiated assess both diversity evolutionary background circulating CPXVs. Here we report full-length sequences 20 strains isolated from animal humans in Germany. A...
Abstract Emerging variants of concern (VOCs) are driving the COVID-19 pandemic 1,2 . Experimental assessments replication and transmission major VOCs progenitors needed to understand mechanisms 3 Here we show that spike protein (S) from Alpha (also known as B.1.1.7) Beta (B.1.351) had a greater affinity towards human angiotensin-converting enzyme 2 (ACE2) receptor than progenitor variant S(D614G) in vitro. Progenitor virus expressing (wt-S 614G ) showed similar kinetics nasal airway...