A5046671398- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
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- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Physics and Engineering Research Articles
- Engineering and Materials Science Studies
- Vector-Borne Animal Diseases
- Criminal Law and Policy
- Viral Infections and Immunology Research
- Inorganic and Organometallic Chemistry
- Animal Virus Infections Studies
- Toxin Mechanisms and Immunotoxins
- Plant Virus Research Studies
- Virus-based gene therapy research
- Influenza Virus Research Studies
- Toxoplasma gondii Research Studies
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Eurasian Exchange Networks
- Chemistry and Chemical Engineering
- Viral-associated cancers and disorders
- Linguistics and Cultural Studies
- Vector-borne infectious diseases
- Coal and Coke Industries Research
Friedrich-Loeffler-Institut
2015-2025
Walter de Gruyter (Germany)
2020-2023
Berlin School of Economics and Law
2021-2023
Max Planck Institute for Legal History and Legal Theory
2020-2023
International Business School
2020-2022
Institute for the Sociology of Law and Criminology
2016-2021
Institute of Molecular Biology
2002-2014
Praxis
2014
Animal Health Research Institute
2011
Institute of Molecular Biology
2000-2010
ABSTRACT Egress of four important alphaherpesviruses, equine herpesvirus 1 (EHV-1), herpes simplex virus type (HSV-1), infectious laryngotracheitis (ILTV), and pseudorabies (PrV), was investigated by electron microscopy infected cell lines different origins. In all virus-cell systems analyzed, similar observations were made concerning the stages virion morphogenesis. After intranuclear assembly, nucleocapsids bud at inner leaflet nuclear membrane, resulting in enveloped particles perinuclear...
ABSTRACT A 2.6-kbp fragment of the pseudorabies virus (PrV) genome was sequenced and shown to contain homologues highly conserved herpesvirus genes UL31 UL32. By use a monospecific antiserum, gene product identified as nuclear protein with an apparent molecular mass 29 kDa. For functional analysis, deleted by mutagenesis in Escherichia coli infectious full-length clone PrV genome. The resulting mutants were deficient plaque formation, titers reduced more than 100-fold from those wild-type...
Newcastle disease virus (NDV)-expressing avian influenza (AIV) hemagglutinin (HA) of subtype H5 was constructed by reverse genetics. A cloned full-length copy the genome lentogenic NDV strain Clone 30 used for insertion ORF encoding HA highly pathogenic AIV isolate A/chicken/Italy/8/98 (H5N2) in intergenic region between fusion and hemagglutinin-neuraminidase (HN) genes. Remarkably, two species transcripts were detected cells infected with resultant NDVH5. In a second recombinant (NDVH5m),...
Although the nuclear envelope is a dynamic structure that disassembles and reforms during mitosis, formation of membranous vesicles derived from has not yet been described in noninfected cells. However, herpesvirus maturation, intranuclear capsids initiate transit to cytosol for final maturation by budding at inner membrane. Two conserved proteins are required this primary envelopment, designated alphaherpesviruses as pUL31 pUL34. Here, we show simultaneous expression pUL34 alphaherpesvirus...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTheory of Coal PyrolysisWalter Fuchs and A. G. SandhoffCite this: Ind. Eng. Chem. 1942, 34, 5, 567–571Publication Date (Print):May 1, 1942Publication History Published online1 May 2002Published inissue 1 1942https://pubs.acs.org/doi/10.1021/ie50389a010https://doi.org/10.1021/ie50389a010research-articleACS PublicationsRequest reuse permissionsArticle Views1543Altmetric-Citations85LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum...
ABSTRACT The UL36 open reading frame encoding the tegument protein ICP1/2 represents largest in genome of herpes simplex virus type 1 (HSV-1). Polypeptides homologous to HSV-1 are present all subfamilies Herpesviridae . We sequenced gene alphaherpesvirus pseudorabies (PrV) and prepared a monospecific polyclonal rabbit antiserum against bacterial glutathione S -transferase (GST)-UL36 fusion for identification protein. detected >300-kDa PrV-infected cells purified virions. Interestingly,...
ABSTRACT Envelope glycoprotein M (gM) and the complex formed by glycoproteins E (gE) I (gI) are involved in secondary envelopment of pseudorabies virus (PrV) particles cytoplasm infected cells. In absence gE-gI gM, is blocked capsids surrounded tegument proteins accumulate (A. R. Brack, J. Dijkstra, H. Granzow, B. G. Klupp, T. C. Mettenleiter, Virol. 73: 5364-5372, 1999). Here we demonstrate yeast two-hybrid analyses that cytoplasmic domains gE gM specifically interact with C-terminal part...
The pseudorabies virus (PrV) homolog of the tegument protein encoded by UL48 gene herpes simplex type 1 (HSV-1) was identified using a monospecific rabbit antiserum against bacterial fusion protein. UL48-related polypeptides 53, 55, and 57 kDa were detected in Western blots infected cells purified virions. Immunofluorescence studies demonstrated that PrV is predominantly localized cytoplasm but also found nuclei cells. Moreover, it constituent extracellular particles absent from primary...
ABSTRACT The large tegument protein encoded by the UL36 gene of pseudorabies virus (PrV) physically interacts with product adjacent UL37 (B. G. Klupp, W. Fuchs, H. Granzow, R. Nixdorf, and T. C. Mettenleiter, J. Virol. 76: 3065-3071, 2002). To analyze function, two PrV recombinants were generated mutagenesis an infectious full-length clone in Escherichia coli : PrV-ΔUL36F exhibited a deletion virtually complete coding region, whereas PrV-UL36BSF contained in-frame deletions 238 codons...
Homologs of the small tegument protein encoded by UL11 gene herpes simplex virus type 1 are conserved throughout all herpesvirus subfamilies. However, their function during viral replication has not yet been conclusively shown. Using a monospecific antiserum and an appropriate deletion rescue mutant, we identified functionally characterized alphaherpesvirus pseudorabies (PrV). PrV encodes with apparent molecular mass 10 to 13 kDa that is primarily detected at cytoplasmic membranes...
In the current perception of herpesvirus replication cycle, two fusion processes are thought to occur during entry and nuclear egress. For penetration, glycoproteins gB gH/gL have been shown be essential, whereas a possible role these in egress remains unclear. Viral envelope detected by immunolabeling membrane as well primary enveloped particles several herpesviruses, indicating that they might involved process. Moreover, herpes simplex virus type 1 mutant simultaneously lacking gH was...
(1953). Was South Africa already known in the 13th century? Imago Mundi: Vol. 10, No. 1, pp. 50-50.
nichsten Tage morde mit 4 03 ccm 0.09653 n.Salpetersaure zuriicktitriert.Das Lacton verbrauchlc zur Verseifung 13.91 Natron, bcr.13.87 ccm.a-Athyl-P-butyrolacton ist eine farblose,
Belranntlich ist es Adolf v. B a e y r nicht gelungen, die von ihm beim Phloroglucin entdeclrte Tautomerie-Erscheinung Resorcin viederzufinden 3. Wir glauben nun, rnit vorliegender Mitteilung d i 'I'automerie s R o c n erweisen zu konnen.Das Mittel hierzu bietet Einwirlrung yon N t u m b l f auf dieses Phenol.Die Einwirkung Natriumbisulfit nuf bereits 1.011.B h 3 ) studiert worden.Unsere Arbeitsweise stimmte der seinigen dorchaus iiberein j sie bestand
Infectious laryngotracheitis virus (ILTV), a member of the Alphaherpesvirinae , possesses several unique genes. One them, UL0, encodes an abundantly expressed protein that accumulates in nuclei ILTV-infected cells. This study demonstrates this is dispensable for vitro replication and UL0 deletion mutants exhibit only minor growth defects cultured The gene locus ILTV was also used insertion foreign DNA sequences encoding enhanced GFP or haemagglutinin (HA), subtype H7, highly pathogenic avian...
ABSTRACT Proteins encoded by the UL46 and UL47 genes of herpes simplex virus type 1 (HSV-1) constitute major components viral tegument. However, their functions have so far not been elucidated in detail. By use monospecific antisera directed against bacterially expressed glutathione- S -transferase fusion proteins, homologous proteins alphaherpesvirus pseudorabies (PrV) were identified virus-infected cells virions. The PrV gene product 693 amino acids (aa) exhibits an apparent molecular mass...
ABSTRACT The pseudorabies virus (PrV) proteins UL11, glycoprotein E (gE), and gM are involved in secondary envelopment of tegumented nucleocapsids the cytoplasm. To assess relative contributions these to process, mutants with deletions either gM, or gE as well two newly constructed mutant viruses simultaneous UL11 were analyzed cell culture for their growth phenotype. We show here that deletion reduced plaque size an additive manner over reduction observed by only gE. However, one-step was...
ABSTRACT The conserved membrane-associated tegument protein pUL11 and envelope glycoprotein M (gM) are involved in secondary envelopment of herpesvirus nucleocapsids the cytoplasm. Although deletion either gene had only moderate effects on replication related alphaherpesviruses herpes simplex virus type 1 (HSV-1) pseudorabies (PrV) cell culture, simultaneous both genes resulted a severe impairment virion morphogenesis PrV coinciding with formation huge inclusions cytoplasm containing...
Vaccination is the best form of protecting fish against viral diseases when pathogen cannot be contained by biosecurity measures. Vaccines based on live attenuated viruses seem to most effective for vaccination challenging pathogens like Cyprinid herpesvirus 3 . However, there are still knowledge gaps how these vaccines effectively protect from deadly disease caused epitheliotropic CyHV-3, and which aspects non-direct protection skin or gill integrity function important in aquatic...