A5059792777- Trace Elements in Health
- Environmental Toxicology and Ecotoxicology
- Mercury impact and mitigation studies
- Heavy Metal Exposure and Toxicity
- Heavy metals in environment
- Freshwater macroinvertebrate diversity and ecology
- Iron Metabolism and Disorders
- Parasite Biology and Host Interactions
- RNA and protein synthesis mechanisms
- Invertebrate Immune Response Mechanisms
- Marine Biology and Environmental Chemistry
- Identification and Quantification in Food
- Nanoparticles: synthesis and applications
- Environmental DNA in Biodiversity Studies
- Physiological and biochemical adaptations
- Neurobiology and Insect Physiology Research
- Selenium in Biological Systems
- Heavy Metals in Plants
- Fish Ecology and Management Studies
- Metalloenzymes and iron-sulfur proteins
- Corrosion Behavior and Inhibition
- Mollusks and Parasites Studies
- Microplastics and Plastic Pollution
- Echinoderm biology and ecology
- Enzyme Structure and Function
Universität Innsbruck
2015-2024
The aim of this study was to analyze the expression three metallothionein (MT) isoform genes (CdMT, CuMT and Cd/CuMT), already known from adults, in Early Life Stage (ELS) Cantareus aspersus. This accomplished by detection MT isoform-specific transcription adopting Polymerase Chain Reaction (PCR) amplification quantitative Real Time (qRT)-PCR genes. Freshly laid eggs were kept for 24 hours under control conditions or exposed cadmium (Cd) solutions increasing concentration (5, 10, 15 mg...
Metal detoxification is crucial for animals to cope with environmental exposure. In snails, a pivotal role in protection against cadmium (Cd) attributed metallothioneins (MTs). Some gastropod species express, lineage-specific manner, Cd-selective MTs devoted exclusively the binding and of this single metal, whereas other snails possess non-selective MTs, but still show high tolerance Cd. An explanation may be that invertebrates particular also synthetize phytochelatins (PCs), originally...
The terrestrial Roman snail Helix pomatia has successfully adapted to strongly fluctuating conditions in its natural soil habitat. Part of the snail's stress defense strategy is ability express Metallothioneins (MTs). These are multifunctional, cysteine-rich proteins that bind and inactivate transition metal ions (Cd(2+), Zn(2+), Cu(+)) with high affinity. In a Cadmium (Cd)-selective, inducible Metallothionein Isoform (CdMT) mainly involved detoxification this harmful metal. addition, CdMT...
Abstract The tiny contribution of cadmium (Cd) to the composition earth’s crust contrasts with its high biological significance, owing mainly competition Cd essential zinc (Zn) for suitable metal binding sites in proteins. In this context it was speculated that several animal lineages, protein family metallothioneins (MTs) has evolved specifically detoxify Cd. Although multi-functionality and heterometallic MTs most species does not support such an assumption, there are some exceptions role,...
Metallothioneins (MTs) are proteins devoted to the control of metal homeostasis and detoxification, therefore, MTs have been crucial for adaptation living beings variable situations bioavailability. The evolution is, however, not yet fully understood, provide new insights into it, we investigated in diverse classes Mollusks. We shown that most molluskan bimodular combine six domains-α, β1, β2, β3, γ, δ-in a lineage-specific manner. functionally characterized Neritimorpha β3β1...
<italic>Arion vulgaris</italic>is a European slug with huge potential for accumulating and detoxifying heavy metals.
Metallothioneins (MTs) are low-molecular-mass, cysteine-rich, metal binding proteins. In most animal species, they involved in homeostasis and detoxification, provide protection from oxidative stress. Gastropod MTs highly diversified, exhibiting unique features adaptations like specificity multiplications of their domains. Here, we show that the MT gene
Terrestrial snails (Gastropoda) possess Cd-selective metallothioneins (CdMTs) that inactivate Cd2+ with high affinity. Most of these MTs are small Cysteine-rich proteins bind 6 equivalents within two distinct metal-binding domains, a binding stoichiometry 3 ions per domain. Recently, unusually large, so-called multi-domain (md-MTs) were discovered in the terrestrial door snail Alinda biplicata (A.b.). The aim this study is to evaluate ability A.b. cope Cd stress and potential involvement...
Abstract Terrestrial gastropods express metal‐selective metallothioneins (MTs) by which they handle metal ions such as Zn 2+ , Cd and Cu + /Cu through separate metabolic pathways. At the same time, depend on availability of sufficient amounts an essential constituent their respiratory protein, hemocyanin (Hc). It was, therefore, suggested that in snails Cu‐dependent MT Hc pathways might be metabolically connected. In fact, Cu‐specific snail (CuMT) is exclusively expressed rhogocytes, a...
The Great Pond snail Lymnaea stagnalis (Gastropoda, Hygrophila) is a wide-spread freshwater gastropod, being considered as model organism for research in many fields of biology, including ecotoxicology. aim the present study was to explore Cd sensitivity L. through measurement biomarker battery oxidative, toxic and cellular stress. interpretation parameters occurred against background truncated metallothionein protein with limited Cd-binding capacity. Individuals were exposed 14 days...
Diamesa species (Diptera, Chironomidae) are widely distributed in freshwater ecosystems, and their life cycles closely linked to environmental variables such as temperature, water quality, sediment composition. Their sensitivity changes, particularly response pollution habitat alterations, makes them valuable indicators of ecosystem health. The challenges associated with the morphological identification larvae invoke use DNA barcoding for determination. mitochondrial cytochrome oxidase...
Chironomids (non-biting midges) inhabit almost every wet or semi-wet continental environment on Earth with probably 10,000 different species. Species occurrence and composition are undoubtedly limited by environmental harshness food availability being reflected in their energy stores. Most animals store as glycogen lipid. They enable the to survive adverse situations continue growth, development, reproduction. This general statement is also true for insects particularly chironomid larvae....
Metal regulation is essential for terrestrial gastropods to survive. In helicid snails, two metal-selective metallothionein (MT) isoforms with different functions are expressed. A cadmium-selective isoform (CdMT) plays a major role in Cd2 + detoxification and stress response, whereas copper-selective MT (CuMT) involved Cu homeostasis hemocyanin synthesis. third, non-metal-selective isoform, called Cd/CuMT, was first characterized Cantareus aspersus. The aim of this study quantify the...
Metallothioneins’ (MTs) biological function has been a matter of debate since their discovery. The importance to categorize these cysteine-rich proteins with high coordinating capacity into specific group led numerous classification proposals. We proposed based on metal-binding abilities, gradually sorting them from those selectivity towards Zn/Cd that are Cu-specific. However, the study NpeMT1 and NpeMT2isoforms Nerita peloronta, put new perspective this classification. N. peloronta chosen...
Protein domains are independent structural and functional modules that can rearrange to create new proteins. While the evolution of multidomain proteins through shuffling different preexisting has been well documented, domain repeat origin less understood. Metallothioneins (MTs) provide a good case study considering they consist metal-binding repeats, some them with likely de novo origin. In mollusks, for instance, most MTs bidomain arose by lineage-specific rearrangements between six...
Abstract Metallothioneins (MTs) constitute an important family of metal binding proteins. Mollusk MTs, in particular, have been used as model systems to better understand the evolution their features and functional adaptation. In present study two recombinantly produced LgiMT1 LgiMT2, de novo evolved γ domain, marine limpet Lottia gigantea , were analyzed by electronic spectroscopy mass spectrometry. Both MT proteins, well domains, exhibit a strong specificity for Cd(II), but not Zn(II) or...
We show for the first time glycosylation of recombinant metallothioneins (MTs) produced in E. coli. Interestingly, our results that level MTs is inversely proportional to degree protein structuration, and reflects their different metal preferences.
Abstract Background Hemocyanin is the oxygen transporter of most molluscs. Since affinity hemocyanin strongly temperature-dependent, this essential protein needs to be well-adapted environment. In Tectipleura, a very diverse group gastropods with > 27,000 species living in all kinds habitats, several genes have already been analyzed. Multiple independent duplications gene identified and may represent potential adaptations different environments lifestyles. The aim study further explore...
Metallothioneins (MTs) are a family of mostly low-molecular weight, cysteine-rich proteins capable specific metal-ion binding that involved in metal detoxification and homeostasis, as well stress response. In contrast to most other animal species which possess two-domain (bidominial) MTs, some gastropod have evolved Cd
Abstract Protein modularity is acknowledged for promoting the emergence of new protein variants via domain rearrangements. Metallothioneins (MTs) offer an excellent model system experimentally examining consequences rearrangements due to possibility assess functional properties native and artificially created using spectroscopic methods metal tolerance assays. In this study, we have investigated AbiMT4 from snail Alinda biplicata (Gastropoda, Mollusca), a large MT comprising 10 putative β...