A5010335172- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Molecular Biology Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Analytical Chemistry and Chromatography
- Isotope Analysis in Ecology
- Esophageal Cancer Research and Treatment
- Nuclear Physics and Applications
- Immune cells in cancer
- Advanced Biosensing Techniques and Applications
- Cancer, Lipids, and Metabolism
- Enzyme Structure and Function
- Bioinformatics and Genomic Networks
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Glycosylation and Glycoproteins Research
- Trace Elements in Health
- Cell Image Analysis Techniques
- Thyroid Cancer Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- HER2/EGFR in Cancer Research
Maastricht University
2016-2025
Netherlands Metabolomics Centre
2014-2016
Leiden University Medical Center
2013-2016
Leiden University
2014
Helmholtz Zentrum München
2010-2013
Technical University of Munich
2009-2012
Klinikum rechts der Isar
2010-2012
University Hospital Heidelberg
2012
Center for Environmental Health
2012
Heidelberg University
2012
Clinical laboratory testing for HER2 status in breast cancer tissues is critically important therapeutic decision making. Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) a powerful tool investigating proteins through the direct and morphology-driven analysis of tissue sections. We hypothesized that MALDI-IMS may determine directly from tissues. Breast (n = 48) predefined were subjected to MALDI-IMS, protein profiles obtained Protein identification was...
Screening for colorectal cancer (CRC) has shown to reduce cancer-related mortality, however, acceptance and compliance current programmes are poor. Developing new, more acceptable non-invasive tests the detection of cancerous precancerous lesions would not only allow preselection individuals colonoscopy, but may also prevent by removal lesions. Plasma from 128 (cohort I - exploratory study: 73 cases / 55 controls) was used test performance a single marker, SEPT9, using real-time quantitative...
Chemotherapy for advanced colorectal cancer leads to improved survival; however, predictors of response systemic treatment are not available. Genomic and epigenetic alterations the gene encoding transcription factor AP-2 epsilon (TFAP2E) common in human cancers. The dickkopf homolog 4 protein (DKK4) is a potential downstream target TFAP2E has been implicated chemotherapy resistance. We aimed further evaluate role DKK4 as chemotherapy.
The identification of tumor subpopulations that adversely affect patient outcomes is essential for a more targeted investigation into how tumors develop detrimental phenotypes, as well personalized therapy. Mass spectrometry imaging has demonstrated the ability to uncover molecular intratumor heterogeneity. challenge been conduct an objective analysis resulting data identify those outcome. Here we introduce spatially mapped t-distributed stochastic neighbor embedding (t-SNE), nonlinear...
Abstract We present the first analytical approach to demonstrate in situ imaging of metabolites from formalin‐fixed, paraffin‐embedded ( FFPE ) human tissue samples. Using high‐resolution matrix‐assisted laser desorption/ionization Fourier‐transform ion cyclotron resonance mass spectrometry MALDI‐FT‐ICR MSI ), we conducted a proof‐of‐principle experiment comparing metabolite measurements and fresh frozen sections, found an overlap 72% amongst 1700 m/z species. In particular, observed...
In clinical diagnostics, it is of outmost importance to correctly identify the source a metastatic tumor, especially if no apparent primary tumor present. Tissue-based proteomics might allow correct classification. As result, we performed MALDI imaging generate proteomic signatures for different tumors. These were used classify common cancer types. At first, cohort comprised tissue samples from six adenocarcinoma entities located at organ sites (esophagus, breast, colon, liver, stomach,...
Abstract Regional lymph node metastasis negatively affects prognosis in colon cancer patients. The molecular processes leading to regional are only partially understood and proteomic markers for still scarce. Therefore, a tissue‐based approach was undertaken identifying proteins associated with metastasis. Two complementary methods have been employed. MALDI imaging used small (≤25 kDa) situ label‐free quantitative proteomics larger proteins. A tissue cohort comprising primary tumours without...
Abstract An essential and so far unresolved factor influencing the evolution of cancer clinical management patients is intratumour clonal phenotypic heterogeneity. However, de novo identification tumour subpopulations both a challenging an task. Here we present first systematic approach for discovery clinically detrimental molecular subpopulations. In this proof‐of‐principle study, spatially resolved, tumour‐specific mass spectra were acquired, using matrix‐assisted laser...
ADVERTISEMENT RETURN TO ISSUEPREVFeatureNEXTCurrent State and Future Challenges of Mass Spectrometry Imaging for Clinical ResearchThe ability mass spectrometry imaging (MSI) to localize panels biomolecules in tissues, without prior knowledge their presence a label-free manner, has led rapid substantial impact clinical pharmacological research, uncovering biomolecular changes associated with disease providing low cost pharmaceuticals. This Feature article will give an introduction the...
Abstract Background Prostate cancer tissues are inherently heterogeneous, which presents a challenge for metabolic profiling using traditional bulk analysis methods that produce an averaged profile. The aim of this study was therefore to spatially detect metabolites and lipids on prostate tissue sections by mass spectrometry imaging (MSI), method facilitates molecular heterogeneous sections, can subsequently be related the histology same section. Methods Here, we simultaneously obtained...
BACKGROUND. Although aberrant glycosylation is recognized as a hallmark of cancer, in clinical breast cancer (BC) metastasis has not yet been studied. While preclinical studies show that the glycocalyx coating cells involved adhesion, migration, and metastasis, changes from primary tumor (PT) to various metastatic sites remain unknown patients.
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful tool for the visualization of proteins in tissues and has demonstrated considerable diagnostic prognostic value. One main challenge that molecular identity such potential biomarkers mostly remains unknown. We introduce generic method removes this issue by systematically identifying embedded MALDI matrix using combination bottom-up top-down proteomics. The analyses ten human lead to identification...
Chemotherapeutic drugs kill cancer cells, but it is unclear why this happens in responding patients not non-responders. Proteomic profiles of with oesophageal adenocarcinoma may be helpful predicting response and selecting more effective treatment strategies. In study, pretherapeutic biopsies were analysed for proteomic changes associated to chemotherapy by MALDI imaging mass spectrometry. Resulting candidate proteins identified liquid chromatography-tandem spectrometry (LC-MS/MS)...
MALDI mass spectrometry imaging (MSI) has rapidly established itself as a powerful biomarker discovery tool. To date, no formal investigation assessed the center-to-center comparability of MSI experiments, an essential step for it to develop into new diagnostic method. test such capabilities, we have performed multicenter study focused on biomarkers stromal activation in breast cancer. experiments were two centers using independent tissue banks, infrastructure, methods, and practitioners....
The combination of mass spectrometry imaging and histology has proven a powerful approach for obtaining molecular signatures from specific cells/tissues interest, whether to identify biomolecular changes associated with histopathological entities or determine the amount drug in organs/compartments. Currently there is no software that able explicitly register data spanning different ionization techniques analyzers. Accordingly, full capabilities are at present underexploited. Here we fully...
Abstract Metabolites, lipids, and other small molecules are key constituents of tissues supporting cellular programs in health disease. Here, we present METASPACE, a community-populated knowledge base spatial metabolomes from imaging mass spectrometry data. METASPACE is enabled by high-performance engine for metabolite annotation confidence-controlled way that makes results comparable between experiments laboratories. By sharing their publicly, users continuously populate annotated currently...
Abstract The large‐scale and label‐free molecular characterization of single cells in their natural tissue habitat remains a major challenge biology. We present method that integrates morphometric image analysis to delineate classify individual with single‐cell‐specific profiles. This approach provides new means study spatial biological processes such as cancer field effects the relationship between features.
Cells often adopt different phenotypes, dictated by tissue-specific or local signals such as cell-cell and cell-matrix contacts molecular micro-environment. This holds in extremis for macrophages with their high phenotypic plasticity. Their broad range of functions, some even opposing, reflects heterogeneity, a multitude subsets has been described tissues diseases. Such micro-environmental imprint cannot be adequately studied single-cell applications, cells are detached from context, while...
BACKGROUND: The metabolic alterations occurring within the arterial architecture during atherosclerosis development remain poorly understood, let alone those particular to each tunica. We aimed first identify, in a spatially resolved manner, specific changes plaque, media, adventitia, and cardiac tissue between control atherosclerotic murine aortas. Second, we assessed their translatability human plasma for cardiovascular risk estimation. METHODS: In this observational study, mass...