A5016941065- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Coronary Interventions and Diagnostics
- Mechanical Circulatory Support Devices
- Nitric Oxide and Endothelin Effects
- Microfluidic and Capillary Electrophoresis Applications
- Congenital heart defects research
- Microfluidic and Bio-sensing Technologies
- Immune cells in cancer
- Transplantation: Methods and Outcomes
- Nanoparticles: synthesis and applications
- Peripheral Artery Disease Management
- Cell Adhesion Molecules Research
- Cardiac Arrest and Resuscitation
- Neuroinflammation and Neurodegeneration Mechanisms
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cardiac and Coronary Surgery Techniques
- Cellular Mechanics and Interactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Anesthesia and Neurotoxicity Research
- Heavy Metal Exposure and Toxicity
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2004-2024
CEA Grenoble
2001-2024
Université Paris Cité
2017-2024
Inserm
2017-2024
Institut de Recherche Interdisciplinaire de Grenoble
2020-2024
University of Prishtina
2021-2023
Hôpital Saint-Louis
2017-2022
Assistance Publique – Hôpitaux de Paris
2020-2022
Université Paris-Saclay
2021
Délégation Ile-de-France Villejuif
2020
Severe quantitative loss of protein is often observed in high-resolution two-dimensional electrophoresis membrane proteins, while the resolution usually not affected. To improve solubility proteins this technique, we tested denaturing cocktails containing various detergents and chaotropes. Best results were obtained with a solution urea, thiourea, zwitterionic detergents, synthesized for purpose. Among dozen tested, amidosulfobetaines an alkyl tail 14-16 carbons proved most efficient,...
We demonstrate the optical manipulation of cells and dielectric particles on surface silicon nitride waveguides. Glass with 2microm diameter are propelled at velocities 15microm/s a guided power 20mW. This is approximately 20 times more efficient than previously reported, permits to use this device low refractive index objects such as cells. Red blood yeast can be trapped waveguide pushed along it by action forces. kind system easily combined various integrated structures opens way...
Purpose To identify prognostic factors of survival in pediatric post-transplantation lymphoproliferative disorder (PTLD) after solid organ transplantation. Patients and Methods A multicenter, retrospective case analysis 55 graft recipients (kidney, liver, heart/lung) developing PTLD were reported to the German Pediatric-PTLD registry. Patient charts analyzed for tumor characteristics (histology, immunophenotypes, cytogenetics, Epstein-Barr virus [EBV] detection), stage, treatment, outcome....
Tissue engineering holds great promise for regenerative medicine, drug discovery, and as an alternative to animal models. However, soon the dimensions of engineered tissue exceed diffusion limit oxygen nutriments, a necrotic core forms leading irreversible damage. To overcome this constraint, establishment functional perfusion network is essential. In work, digital light processing bioprinting used encapsulate endothelial progenitor cells (EPCs) in 3D light-cured hydrogel scaffolds guide...
The superoxide-generating NADPH oxidase complex of phagocytic cells is a multicomponent system containing membrane-bound flavocytochrome b and small G protein Rac as well cytosolic factors p67phox(phagocyte oxidase), p47phox, p40phox, which translocate to the membrane upon activation. In previous paper, we reported that p40phox undergoes phosphorylation on multiple sites stimulation by either phorbol 12-myristate 13-acetate or formyl peptide with time course strongly correlated superoxide...
Sorting and recovering specific live cells from samples containing less than a few thousand have become major hurdles in rare cell exploration such as stem research, therapy based diagnostics. We describe here new technology on microelectronic chip integrating an array of over 100,000 independent electrodes sensors which allow individual parallel single manipulation up to 10,000 while maintaining viability proliferation capabilities. Manipulation is carried out using dynamic...
Increased production and use of zinc oxide nanoparticles (ZnO-NPs) in consumer products has prompted the scientific community to investigate their potential toxicity, understand impact on environment organisms. Molecular mechanisms involved ZnO-NP toxicity are still under debate focus essentially high dose expositions. In our study, we chose evaluate effect sub-toxic doses ZnO-NPs human hepatocytes (HepG2) with a metal homeostasis redox balance disruptions. We showed massive dissolution...
Gene therapy strategies for the prevention of restenosis postangioplasty are promising. Nonviral gene transfer to arterial wall in vivo has so far been limited by poor efficiency. This study aimed optimize transfection primary vascular smooth muscle cells using cationic nonviral formulations based on cholesterol derivates (DC-, DAC-, DCQ-, and Sp-Chol), double-chained amphiphils (LipofectAMINE, DOTMA, DOSGA, DOSPER, DOCSPER), or heterogeneous reagents (Superfect, Effectene, Tfx-50)....
The superoxide-generating NADPH oxidase complex in phagocytic cells is constituted of a heterodimeric flavocytochrome b and cytosolic factors, p67phox, p47phox p40phox as well small G protein Rac (for review, see (1Morel F. Doussière J. Vignais P.V. Eur. Biochem. 1991; 201: 523-546Crossref PubMed Scopus (523) Google Scholar, 2Babior B. Adv. Enzymol. Relat. Areas Mol. Biol. 1992; 65: 49-95PubMed 3Thrasher A.J. Keep N.H. Wientjes Segal A.W. Biochim. Biophys. Acta. 1994; 1227: 1-24Crossref...
The leukocyte NADPH oxidase catalyzes the production of O2- from oxygen at expense NADPH. Activation enzyme requires interaction cytosolic factors p47PHOX, p67PHOX, and Rac2 with membrane-associated cytochrome b558. in a semirecombinant cell-free system absence an amphiphilic activator can be achieved by phosphorylation factor p47PHOX protein kinase C. Another factor, p40PHOX, was recently shown to phosphorylated on serine threonine residues upon activation oxidase, but both stimulatory...
We have previously shown that the injury to cultured liver endothelial cells during cold incubation in University of Wisconsin (UW) solution is energy-dependent and mediated by reactive oxygen species. Here we demonstrate this oxygen-mediated specific neither nor UW solution: hepatocytes incubated (4 degrees C) or histidine-tryptophan-ketoglutarate (HTK) were injured under normoxic conditions (loss viability, 63% +/- 10% after 48 hours 82% 11% 24 HTK solution), whereas hypoxia was protective...
Upon exposure to non-toxic concentration of silver nanoparticles, only the ionic form is found in nucleus leading an endocrine disruptor-like effect.
The superoxide‐generating NADPH oxidase complex of phagocytic cells is a multicomponent system containing membrane‐bound flavocytochrome b and small G protein Rac as well cytosolic factors p67phox, p47phox p40phox which translocate to the membrane upon activation. Known mechanisms underlying translocation these proteins include polyphosphorylation specific Src homology 3/polyproline motif interactions. In this study, through two‐dimensionnal electrophoresis immunoprecipitation experiments,...
The neutrophil NADPH oxidase activation factors, p47, p67 and the small guanosine‐nucleotide‐binding regulatory (G) protein Rac1, were expressed in a baculovirus/insect cell system purified. In coinfection experiments which Sf9 cells overexpressed concomitantly latter was not detected p47–p67 complex. propensity of p47 to associate together used purify recombinant from baculovirus–infected cells. 20% Rac1 infected prenylated extracted with low doses detergent membranes. Elicitation full...
Many tissue engineering approaches are being explored to offer solutions for diseased esophageal and its repair. However, classical techniques in not capable of recapitulating the structural functional parameters native esophagi. Esophageal 3D bioprinting is yet an emerging field but holds great promise meeting this challenge. Herein, use extrusion‐based printing examined generate substitutes from a polymer blend‐based formulation. The fabricated structures printed at very high speed without...
Cholangiocytes, biliary epithelial cells, are known to spontaneously self-organize into spherical cysts with a central lumen. In this work, we explore promising biocompatible stereolithographic approach encapsulate cholangiocytes geometrically controlled 3D hydrogel structures guide them towards the formation of branched tubular networks. We demonstrate that within appropriate mix hydrogels, normal rat can proliferate, migrate, and organize walls consisting cell monolayer, transport...