A5008654233- Tissue Engineering and Regenerative Medicine
- Microfluidic and Bio-sensing Technologies
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Tracheal and airway disorders
- Esophageal and GI Pathology
- Microfluidic and Capillary Electrophoresis Applications
- Extracellular vesicles in disease
- Neuroscience and Neuropharmacology Research
- Magnetic and Electromagnetic Effects
- Trauma Management and Diagnosis
- biodegradable polymer synthesis and properties
- Cell Adhesion Molecules Research
- Microbial Inactivation Methods
- Analytical Chemistry and Chromatography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Spinal Dysraphism and Malformations
- MicroRNA in disease regulation
- Atherosclerosis and Cardiovascular Diseases
- Amino Acid Enzymes and Metabolism
- Liver Disease Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Congenital Diaphragmatic Hernia Studies
- Mesenchymal stem cell research
- Congenital Anomalies and Fetal Surgery
Hôpital Saint-Louis
2015-2024
Assistance Publique – Hôpitaux de Paris
2016-2024
Université Paris Cité
2018-2024
Inserm
2015-2024
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2023
HIPI - Immunologie humaine, physiopathologie et immunithérapie
2021-2022
Physique et Mécanique des Milieux Hétérogènes
2022
Institut de recherche Saint-Louis
2019-2021
Délégation Paris 7
2018
Centre National de la Recherche Scientifique
2014
Abstract Acoustic levitation, which allows contactless manipulation of micro‐objects with ultrasounds, is a promising technique for spheroids formation and culture. This acoustofluidic favors cell–cell interactions, away from the walls chip, leads to spontaneous self‐organization cells. Using this approach, we generated mesenchymal stromal cells, hepatic endothelial showed that long‐term culture cells in acoustic levitation feasible. We also demonstrated its dynamics depended weakly on...
Abstract In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies shown that cultures exhibit better biomimetic properties compared to 2D cultures. These are for in-vitro modeling systems, well in-vivo therapies and tissue engineering approaches. A reliable use of cellular still requires standardized protocols with well-controlled reproducible parameters. To address this challenge, a robust scaffold-free approach is...
Tissue engineering appears promising as an alternative technique for esophageal replacement. Mesenchymal stem cells (MSCs) could be of interest regeneration. Evaluation the ability acellular matrix seeded with autologous MSCs to promote tissue remodeling toward phenotype after circumferential replacement esophagus in a mini pig model. A 3 cm long abdominal was performed MSC-seeded (MSC group, n = 10) versus alone (control 10), which has previously been matured into great omentum. The graft...
Endothelial cells cover the lining of different blood vessels and lymph nodes, have major functions including transport blood, vessel homeostasis, inflammatory responses, control transendothelial migration circulating into tissues, formation new vessels. Therefore, understanding these is interest. The morphological features, phenotype function endothelial varies according to vascular bed examined. sialomucin, CD34, widely used as an marker. However, CD34 differentially expressed on in organs...
In pathologies of the esophagus such as esophageal atresia, cancers, and caustic injuries, methods for full thickness replacement require sacrifice healthy intra-abdominal organs stomach colon are associated with high morbidity, mortality, poor functional results. To overcome these problems, tissue engineering developed to create a substitute scaffolds cells. The aim this study was develop simple safe decellularization process in order obtain clinical grade extracellular matrix. Following...
Many tissue engineering approaches are being explored to offer solutions for diseased esophageal and its repair. However, classical techniques in not capable of recapitulating the structural functional parameters native esophagi. Esophageal 3D bioprinting is yet an emerging field but holds great promise meeting this challenge. Herein, use extrusion‐based printing examined generate substitutes from a polymer blend‐based formulation. The fabricated structures printed at very high speed without...
Abstract Background Myelomeningocele (MMC) is a spinal cord congenital defect that leads to paraplegia, sphincter disorders and potential neurocognitive disabilities. Prenatal surgery of MMC provides significant benefit compared at birth. Mesenchymal stromal cell (MSC) therapy as an adjuvant treatment for prenatal showed promising results in animal experiments which could be considered clinical use human fetuses. Despite numerous reassuring studies on the safety MSCs administration humans,...
Tissue engineering is a promising alternative to current full thickness circumferential esophageal replacement methods. The aim of our study was develop clinical grade Decellularized Human Esophagus (DHE) for future applications. After decontamination, human esophagi from deceased donors were placed in bioreactor and decellularized with sodium dodecyl sulfate (SDS) ethylendiaminetetraacetic acid (EDTA) 3 days. then rinsed sterile water SDS eliminated by filtration on an activated charcoal...
Abstract The management of extensive tracheal resection followed by circumferential replacement remains a surgical challenge. Numerous techniques are proposed with mixed results. Partial decellularization the trachea removal mucosal and submucosal cells is promising method, reducing immunogenicity while preserving biomechanical properties final matrix. Despite many research protocols proofs concept, no standardized clinical grade protocol described. Furthermore, local systemic biointegration...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
Abstract In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies shown that cultures exhibit better biomimetic properties compared to 2D cultures. These are for in-vitro modeling systems, well in-vivo therapies and tissue engineering approaches. A reliable use of cellular still requires standardized protocols with well-controlled reproducible parameters. To address this challenge, a robust scaffold-free approach is...